View PDF - G3: Genes | Genomes | Genetics
... many generations until a period of senescence/crisis is reached. After this, a small fraction of cells can escape senescence, principally using recombination-dependent mechanisms. To investigate the pathways that affect entry into and recovery from telomere-driven senescence, we combined a gene dele ...
... many generations until a period of senescence/crisis is reached. After this, a small fraction of cells can escape senescence, principally using recombination-dependent mechanisms. To investigate the pathways that affect entry into and recovery from telomere-driven senescence, we combined a gene dele ...
Biology for AIEEE - CET 2009-10
... In a case of disputed parentage, a woman of blood group AB claimed that a baby of blood group O was her child. In this case, which one of the following is true? (a) The baby belongs to her if her husband’s blood group is AB (b) The baby cannot belong to her if her husbands blood group is A (c) The b ...
... In a case of disputed parentage, a woman of blood group AB claimed that a baby of blood group O was her child. In this case, which one of the following is true? (a) The baby belongs to her if her husband’s blood group is AB (b) The baby cannot belong to her if her husbands blood group is A (c) The b ...
Cytoplasmic sequestration of the tumor suppressor p53 by a heat
... HCT-8 and HCT-15 were cultured in RPMI media containing 10% FBS with the HCT-15 cells also receiving 1 mM sodium pyruvate. LS123 was cultured in ATCC-formulated Eagles’ Minimum Essential media (#30–2003). The remaining cell lines were cultured in DMEM/F12 media with 10% FBS and all cell lines were g ...
... HCT-8 and HCT-15 were cultured in RPMI media containing 10% FBS with the HCT-15 cells also receiving 1 mM sodium pyruvate. LS123 was cultured in ATCC-formulated Eagles’ Minimum Essential media (#30–2003). The remaining cell lines were cultured in DMEM/F12 media with 10% FBS and all cell lines were g ...
Sites of Synthesis of Urokinase and Tissue
... tions ofthe respective mRNAs. The u-PA cRNA probe labeled a well-defined zone of maximal intensity, corresponding to the outer stripe of the outer medulla, with a clear delineation of the medullary rays; a somewhat weaker signal was noticed in the inner stripe of the outer medulla, while the inner m ...
... tions ofthe respective mRNAs. The u-PA cRNA probe labeled a well-defined zone of maximal intensity, corresponding to the outer stripe of the outer medulla, with a clear delineation of the medullary rays; a somewhat weaker signal was noticed in the inner stripe of the outer medulla, while the inner m ...
PDF - The Journal of Cell Biology
... Fig. 2 B). Spz is only secreted full length and is cleaved extracellularly by the serine proteases Easter or Spz processing enzyme (Hoffmann and Reichhart, 2002). Furin sites were absent from the Spz prodomain, but several highly conserved sites were found in DNT1 and 2 (Fig. 2 B). In vivo overexpre ...
... Fig. 2 B). Spz is only secreted full length and is cleaved extracellularly by the serine proteases Easter or Spz processing enzyme (Hoffmann and Reichhart, 2002). Furin sites were absent from the Spz prodomain, but several highly conserved sites were found in DNT1 and 2 (Fig. 2 B). In vivo overexpre ...
- Lorentz Center
... fibronectin matrix of the PSM are crucial for the segmentation process to actually take place (Dray et al., 2013; Girós, Grgur, Gossler, & Costell, 2011). This mechanical perspective sheds a new light on molecular studies aimed at alteration of specific gene activities. For instance, the most natural ...
... fibronectin matrix of the PSM are crucial for the segmentation process to actually take place (Dray et al., 2013; Girós, Grgur, Gossler, & Costell, 2011). This mechanical perspective sheds a new light on molecular studies aimed at alteration of specific gene activities. For instance, the most natural ...
Speciation of Small Molecules and Inorganic Ions in Salmon Egg
... (Received September 4, 2002; Accepted October 3, 2002) ...
... (Received September 4, 2002; Accepted October 3, 2002) ...
MORPHOLOGICAL AND FUNCTIONAL PROPERTIES OF
... of the marine gastropod, ApZysia, is a valuable preparation for cellular electrophysiological studies. In large animals, most small nerve cells in this ganglion are comparable in size to the largest neurons in the vertebrate brain (So-100 p), whereas the largest cells in the the majority ganglion oc ...
... of the marine gastropod, ApZysia, is a valuable preparation for cellular electrophysiological studies. In large animals, most small nerve cells in this ganglion are comparable in size to the largest neurons in the vertebrate brain (So-100 p), whereas the largest cells in the the majority ganglion oc ...
Vps41 Phosphorylation and the Rab Ypt7 Control the Targeting of
... Identification of the Yck3 Phosphorylation Site One fundamental problem of our EM analysis is the correlation of the altered endosomal morphology to the function of Vps41 because Yck3 has several other targets within the cell, for example, Vam3 (Brett et al., 2008). To unequivocally link the in vivo ...
... Identification of the Yck3 Phosphorylation Site One fundamental problem of our EM analysis is the correlation of the altered endosomal morphology to the function of Vps41 because Yck3 has several other targets within the cell, for example, Vam3 (Brett et al., 2008). To unequivocally link the in vivo ...
Modulating progenitor accumulation attenuates lung angiogenesis in a mouse model of asthma
... progenitor cells (VEPCs) following allergen challenge in sensitised mice, which was associated with increased lung angiogenesis, considered to be an early remodelling event in asthma [14, 15]. Interestingly, VEPCs express CXCR4 [13], suggesting that SDF-1 may play a pivotal role in the lung homing o ...
... progenitor cells (VEPCs) following allergen challenge in sensitised mice, which was associated with increased lung angiogenesis, considered to be an early remodelling event in asthma [14, 15]. Interestingly, VEPCs express CXCR4 [13], suggesting that SDF-1 may play a pivotal role in the lung homing o ...
G α 12/13 regulate epiboly by inhibiting E
... defect was observed in E-cadherin–deficient embryos, with cells bulging and detaching from the embryonic surface (Babb and Marrs, 2004; Kane et al., 2005; McFarland et al., 2005; Shimizu et al., 2005). E-cadherin is a plasma membrane glycoprotein that is indirectly linked to the actin cytoskeleton t ...
... defect was observed in E-cadherin–deficient embryos, with cells bulging and detaching from the embryonic surface (Babb and Marrs, 2004; Kane et al., 2005; McFarland et al., 2005; Shimizu et al., 2005). E-cadherin is a plasma membrane glycoprotein that is indirectly linked to the actin cytoskeleton t ...
Membrane flow through Golgi compartments
... DOG medium (Fig. 3C). After 30-45 minutes of incubation in DOG medium, the perinuclear reticular structure stained by Helix pomatia disappeared (Fig. 3D,H,I) and a haze of fluorescence was spread throughout most of the area stained with an antibody against the ER marker protein disulphide isomerase ...
... DOG medium (Fig. 3C). After 30-45 minutes of incubation in DOG medium, the perinuclear reticular structure stained by Helix pomatia disappeared (Fig. 3D,H,I) and a haze of fluorescence was spread throughout most of the area stained with an antibody against the ER marker protein disulphide isomerase ...
Mineralogy - Carleton College
... vector c, which is not in the same plane as a and b, I can generate a space lattice ...
... vector c, which is not in the same plane as a and b, I can generate a space lattice ...
- Wiley Online Library
... Figure 2. Mechanoelectrical transducer (MET) current adaptation in apical or basal outer hair cells (OHCs) is similar between Piezo1+/+ and Piezo1+/ mice. A and B, Force step stimuli of 50 ms duration (top) elicited MET currents (bottom panels). Recordings were obtained at 81 mV (left panels) and +9 ...
... Figure 2. Mechanoelectrical transducer (MET) current adaptation in apical or basal outer hair cells (OHCs) is similar between Piezo1+/+ and Piezo1+/ mice. A and B, Force step stimuli of 50 ms duration (top) elicited MET currents (bottom panels). Recordings were obtained at 81 mV (left panels) and +9 ...
Biggins
... poles as the GAL-MPS1 cells escaped from the checkpoint-dependent arrest at 35°C. After 30 min at the nonpermissive temperature, 24% of the GAL-MPS1 and 66% of the GAL-MPS1 ipl1-321 cells had segregated chromosome IV to opposite poles (Fig. 2C). From the fact that chromosome IV segregated to opposit ...
... poles as the GAL-MPS1 cells escaped from the checkpoint-dependent arrest at 35°C. After 30 min at the nonpermissive temperature, 24% of the GAL-MPS1 and 66% of the GAL-MPS1 ipl1-321 cells had segregated chromosome IV to opposite poles (Fig. 2C). From the fact that chromosome IV segregated to opposit ...
The chromatin-remodeling enzyme BRG1 plays an essential role in
... embryos were separated from their placentae by severing the umbilical vessels. The embryos with their attached yolk sacs were immediately placed in minimal essential media containing 2% fetal bovine serum (JRH Biosciences) and were rocked on a Nutator mixer for approximately 30 minutes at room tempe ...
... embryos were separated from their placentae by severing the umbilical vessels. The embryos with their attached yolk sacs were immediately placed in minimal essential media containing 2% fetal bovine serum (JRH Biosciences) and were rocked on a Nutator mixer for approximately 30 minutes at room tempe ...
Power tools for gene expression and clonal analysis in
... for analyzing the temporal requirement of a gene in a fly behavior that may be temperature sensitive as the system can be activated by the addition of quinic acid in the diet rather than by temperature as in the temperature-sensitive GAL80 mutant, GAL80ts (see below). Currently there are few drivers ...
... for analyzing the temporal requirement of a gene in a fly behavior that may be temperature sensitive as the system can be activated by the addition of quinic acid in the diet rather than by temperature as in the temperature-sensitive GAL80 mutant, GAL80ts (see below). Currently there are few drivers ...
Transcription Factor c-Rel B κ Regulation of the IL-21 Gene by the NF-
... Guobing Chen,* Kristine Hardy,* Karen Bunting,*,1 Stephen Daley,† Lina Ma,* and M. Frances Shannon* ...
... Guobing Chen,* Kristine Hardy,* Karen Bunting,*,1 Stephen Daley,† Lina Ma,* and M. Frances Shannon* ...
comparative study of extracellular fibrils on the ectodermal layer in
... R. pipiens with the sperm of Rana catesbeiana. Early gastrulae lack extracellular fibrils. Late gastrulae, however, did have an amorphous layer of extracellular materials coating the inner aspect of the roof of the blastocoel. The scarcity of fibrils inXenopus and the lack of them in Rana, can be in ...
... R. pipiens with the sperm of Rana catesbeiana. Early gastrulae lack extracellular fibrils. Late gastrulae, however, did have an amorphous layer of extracellular materials coating the inner aspect of the roof of the blastocoel. The scarcity of fibrils inXenopus and the lack of them in Rana, can be in ...
The Putative Arabidopsis Arp2/3 Complex
... the leading edge of migrating animal cells (Welch et al., 1997a; Morrell et al., 1999; Schaerer-Brodbeck and Riezman, 2000a, 2000b; Higgs and Pollard, 2001). As a multifunctional organizer, the cellular roles of the Arp2/3 complex involve the control of actin polymerization nucleation for many cell ...
... the leading edge of migrating animal cells (Welch et al., 1997a; Morrell et al., 1999; Schaerer-Brodbeck and Riezman, 2000a, 2000b; Higgs and Pollard, 2001). As a multifunctional organizer, the cellular roles of the Arp2/3 complex involve the control of actin polymerization nucleation for many cell ...
Transcriptional profiling of Arabidopsis root hairs and
... but are an emergent property of the complexity of other underlying levels of organization based on fundamental chemical and physical properties of DNA and proteins. There is no easy way to circumvent these limitations at our present understanding of biology, but usable clues could arise from applyin ...
... but are an emergent property of the complexity of other underlying levels of organization based on fundamental chemical and physical properties of DNA and proteins. There is no easy way to circumvent these limitations at our present understanding of biology, but usable clues could arise from applyin ...
The regulation and function of Class III PI3Ks: novel roles for Vps34
... Methods for measuring Vps34 activity in cells and in vitro are similar to methods used for other PI3K isoforms, and share some of the same limitations; this area has been comprehensively reviewed recently [47]. PtdIns3P, the product of Vps34, can be extracted from [32 P]orthophosphate- or [3 H]myo-i ...
... Methods for measuring Vps34 activity in cells and in vitro are similar to methods used for other PI3K isoforms, and share some of the same limitations; this area has been comprehensively reviewed recently [47]. PtdIns3P, the product of Vps34, can be extracted from [32 P]orthophosphate- or [3 H]myo-i ...
Chapter_4_Tissue_Power_Point
... Tissues of the body develop from three primary germ layers: Ectoderm, Endoderm, and Mesoderm Epithelial tissues develop from all three germ layers All connective tissue and most muscle tissues drive from mesoderm Nervous tissue develops from ectoderm ...
... Tissues of the body develop from three primary germ layers: Ectoderm, Endoderm, and Mesoderm Epithelial tissues develop from all three germ layers All connective tissue and most muscle tissues drive from mesoderm Nervous tissue develops from ectoderm ...
Protein sensing in living cells by molecular rotor
... off/on cycles would present a more desirable feature that can be applied to detect the protein expression level and activity, and also subsequent analysis of protein degradation, translocation and inhibitor screening in cells.8,9 There are many obstacles that need to be addressed in order to construc ...
... off/on cycles would present a more desirable feature that can be applied to detect the protein expression level and activity, and also subsequent analysis of protein degradation, translocation and inhibitor screening in cells.8,9 There are many obstacles that need to be addressed in order to construc ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.