Mastoparan Alters Subcellular Distribution of
... 1998). Importantly, recent genetic studies have revealed that profilin plays critical roles for basic processes of plant development (Ramachandran et al. 2000, McKinney et al. 2001). Profilins from non-plant systems are well known to interact with several classes of proteins that contain long contin ...
... 1998). Importantly, recent genetic studies have revealed that profilin plays critical roles for basic processes of plant development (Ramachandran et al. 2000, McKinney et al. 2001). Profilins from non-plant systems are well known to interact with several classes of proteins that contain long contin ...
Calcium-Dependent Prevention of Neuronal Apoptosis by Lithium Ion
... LY294002, U-73122 and U-73343 were obtained from Calbiochem (La Jolla, CA). Phosphotyrosine antibody 4G10 was obtained from Upstate Biotechnology (Lake Placid, NY). IP3 assay kit was purchased from Amersham Biosciences (Piscataway, NJ). Cell Culture. Cortical cells were prepared from fetal ICR mice ...
... LY294002, U-73122 and U-73343 were obtained from Calbiochem (La Jolla, CA). Phosphotyrosine antibody 4G10 was obtained from Upstate Biotechnology (Lake Placid, NY). IP3 assay kit was purchased from Amersham Biosciences (Piscataway, NJ). Cell Culture. Cortical cells were prepared from fetal ICR mice ...
The Arabidopsis TRM1-TON1 interaction reveals a recruitment
... Apart from proteins of the bona fide g-tubulin complex (Liu et al., 1994; Erhardt et al., 2002; Binarová et al., 2006; Pastuglia et al., 2006; Nakamura and Hashimoto, 2009; Kong et al., 2010), only a handful of plant proteins with similarity with animal centrosomal proteins have been identified and ...
... Apart from proteins of the bona fide g-tubulin complex (Liu et al., 1994; Erhardt et al., 2002; Binarová et al., 2006; Pastuglia et al., 2006; Nakamura and Hashimoto, 2009; Kong et al., 2010), only a handful of plant proteins with similarity with animal centrosomal proteins have been identified and ...
The Arabidopsis TRM1-TON1 interaction reveals a recruitment
... Apart from proteins of the bona fide g-tubulin complex (Liu et al., 1994; Erhardt et al., 2002; Binarová et al., 2006; Pastuglia et al., 2006; Nakamura and Hashimoto, 2009; Kong et al., 2010), only a handful of plant proteins with similarity with animal centrosomal proteins have been identified and ...
... Apart from proteins of the bona fide g-tubulin complex (Liu et al., 1994; Erhardt et al., 2002; Binarová et al., 2006; Pastuglia et al., 2006; Nakamura and Hashimoto, 2009; Kong et al., 2010), only a handful of plant proteins with similarity with animal centrosomal proteins have been identified and ...
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... Planktonic microbial communities often appear stable over periods of days and thus tight links are assumed to exist between different functional groups (i.e. producers and consumers). We examined these links by characterizing short-term temporal correspondences in the concentrations and activities o ...
... Planktonic microbial communities often appear stable over periods of days and thus tight links are assumed to exist between different functional groups (i.e. producers and consumers). We examined these links by characterizing short-term temporal correspondences in the concentrations and activities o ...
ForC, a novel type of formin family protein lacking an FH1 domain, is
... Ax2 cells were allowed to develop on MES agar plates, during which cells were collected from each 100 mm plate every 4 hours. RNA was extracted from the cells using TriZol reagent (Gibco Invitrogen), and was used for synthesis of first strand cDNA using reverse transcriptase (ReverTra Ace; Toyobo) w ...
... Ax2 cells were allowed to develop on MES agar plates, during which cells were collected from each 100 mm plate every 4 hours. RNA was extracted from the cells using TriZol reagent (Gibco Invitrogen), and was used for synthesis of first strand cDNA using reverse transcriptase (ReverTra Ace; Toyobo) w ...
A metabolic perspective of Peto`s paradox and cancer
... If one were to assume that it is true that larger animals have relatively lower rates of cancer burden compared with smaller mammals, could metabolic rates that scale with body size be associated with the propensity for tumorigenesis? The correlation of animal body size with basal metabolic rates ha ...
... If one were to assume that it is true that larger animals have relatively lower rates of cancer burden compared with smaller mammals, could metabolic rates that scale with body size be associated with the propensity for tumorigenesis? The correlation of animal body size with basal metabolic rates ha ...
Text
... peritoneal, pleural and pericardial cavities. These serous cavities constitute important reservoirs of innate-like B-cell subsets, also called B1 cells, the major innate function of which is to ensure early immune protection from infection by rapid secretion of natural IgM. How and where natural IgM ...
... peritoneal, pleural and pericardial cavities. These serous cavities constitute important reservoirs of innate-like B-cell subsets, also called B1 cells, the major innate function of which is to ensure early immune protection from infection by rapid secretion of natural IgM. How and where natural IgM ...
Epstein-Barr virus genetics: talking about the BAC generation Open Access
... and selected for phenotypic characterization. This purification step can only be performed if the mutant has retained its ability to lytically replicate and to infect target cells from which they can be expanded. Therefore, mutant viruses that lack the genetic elements essential for either replicati ...
... and selected for phenotypic characterization. This purification step can only be performed if the mutant has retained its ability to lytically replicate and to infect target cells from which they can be expanded. Therefore, mutant viruses that lack the genetic elements essential for either replicati ...
Database Machine
... Success of SAN caches is built upon predictive analytics They work well, if a small percentage of disk accessed most often - The emphasis is on disk; not data Most database systems - Are way bigger than caches - Need to get the data to the memory to process --> I/O at the disk level is still h ...
... Success of SAN caches is built upon predictive analytics They work well, if a small percentage of disk accessed most often - The emphasis is on disk; not data Most database systems - Are way bigger than caches - Need to get the data to the memory to process --> I/O at the disk level is still h ...
Physiological effects of long-term energy
... stabilized at 0.50 and remained constant throughout the remainder of the study. ATP remained nearly constant for 2 wk then decreased until 4 wk when it stabilized at 0.85 fmol ml-' at 10"ells ml-' N. cryotolerans appears to be well adapted to energy-source deprivation. It is able to conserve energy ...
... stabilized at 0.50 and remained constant throughout the remainder of the study. ATP remained nearly constant for 2 wk then decreased until 4 wk when it stabilized at 0.85 fmol ml-' at 10"ells ml-' N. cryotolerans appears to be well adapted to energy-source deprivation. It is able to conserve energy ...
Molecular Regulation of Vascular Cambium Identity and Activity
... 2006a). Plant stem cells, or the “meristematic cells” or the “initials”, similar to their animal counterparts are reservoirs of cells that can both produce new cells for the generation of new tissues and organs, and self-maintain indeterminately via asymmetric cell division. The asymmetric division ...
... 2006a). Plant stem cells, or the “meristematic cells” or the “initials”, similar to their animal counterparts are reservoirs of cells that can both produce new cells for the generation of new tissues and organs, and self-maintain indeterminately via asymmetric cell division. The asymmetric division ...
Mapping the route from naive pluripotency to lineage specification
... rstb.royalsocietypublishing.org ...
... rstb.royalsocietypublishing.org ...
Extrinsic Factors Influence the Expression of Voltage
... coverslips coated with rat tail collagen and laminin (50 &ml; courtesy of Dr. S. Carbonetto, McGill University). The cultures were maintained for 2-4 weeks at 37°C in 95% air, 5% CO, and fed every 3-l d with the growth media described above. In some experiments, recordings were done on acutely disso ...
... coverslips coated with rat tail collagen and laminin (50 &ml; courtesy of Dr. S. Carbonetto, McGill University). The cultures were maintained for 2-4 weeks at 37°C in 95% air, 5% CO, and fed every 3-l d with the growth media described above. In some experiments, recordings were done on acutely disso ...
- Wiley Online Library
... (Figure 2a–h). Following endocytic uptake of FM4-64 over 20 min using time-lapse confocal microscopy revealed hardly any co-labelling between FM4-64 and YFP–PHFAPP1 (Figure 3 and Movie S2). After 20 min, compartments started to appear closer to each other and sometimes even overlapped (arrowheads in ...
... (Figure 2a–h). Following endocytic uptake of FM4-64 over 20 min using time-lapse confocal microscopy revealed hardly any co-labelling between FM4-64 and YFP–PHFAPP1 (Figure 3 and Movie S2). After 20 min, compartments started to appear closer to each other and sometimes even overlapped (arrowheads in ...
Selection and the Origin of Cells
... how a potentially evolving protocell could arise without any prior selection. It is not enough that its protoplasm have a viable metabolism; the protocell would also need to regulate transport through its bounding membrane, retain metabolically active chemicals, acquire chemical building blocks and ...
... how a potentially evolving protocell could arise without any prior selection. It is not enough that its protoplasm have a viable metabolism; the protocell would also need to regulate transport through its bounding membrane, retain metabolically active chemicals, acquire chemical building blocks and ...
Circuits that build visual cortical receptive fields
... to show that the simple receptive field, as defined by segregated subregions with push–pull structure, is an exclusive feature of the first stage of visual processing (in cats). Cells with simple receptive fields are located in regions where thalamic afferents terminate – that is, layer 4, the borde ...
... to show that the simple receptive field, as defined by segregated subregions with push–pull structure, is an exclusive feature of the first stage of visual processing (in cats). Cells with simple receptive fields are located in regions where thalamic afferents terminate – that is, layer 4, the borde ...
The Hydra
... seven tentacles extending from its mouth. Hydra come in many different colors, including tan, gray, green, and brown. Adult Hydra are typically 6 to 13 millimeters long and are capable of ~tretching out or contracting. ...
... seven tentacles extending from its mouth. Hydra come in many different colors, including tan, gray, green, and brown. Adult Hydra are typically 6 to 13 millimeters long and are capable of ~tretching out or contracting. ...
A model of chloroplast growth regulation in mesophyll cells
... relationships of similar slope, with scatter that scaled with cell size. The transgenic types – with larger and more irregularly shaped chloroplasts than the wild type – presented with more scatter; see the R2 values in Figure 2. Pyke (1999) further showed that this regulated chloroplast density per ...
... relationships of similar slope, with scatter that scaled with cell size. The transgenic types – with larger and more irregularly shaped chloroplasts than the wild type – presented with more scatter; see the R2 values in Figure 2. Pyke (1999) further showed that this regulated chloroplast density per ...
The UDPase activity of the Kluyveromyces lactis Golgi GDPase has
... (ScGda1p) generates GMP, the antiporter required for entry of GDP-mannose, from the cytosol, into the Golgi lumen. Scgda1 deletion strains have severe defects in Nand O-mannosylation of proteins and glycosphingolipids. ScGda1p has also significant UDPase activity even though S. cerevisiae does not u ...
... (ScGda1p) generates GMP, the antiporter required for entry of GDP-mannose, from the cytosol, into the Golgi lumen. Scgda1 deletion strains have severe defects in Nand O-mannosylation of proteins and glycosphingolipids. ScGda1p has also significant UDPase activity even though S. cerevisiae does not u ...
Canonical Wnt9b signaling balances progenitor cell
... homogenized into a single-cell suspension. Nuclei from crosslinked cells were resuspended in Tris-EDTA buffer and sonicated. The soluble chromatin was transferred into radioimmunoprecipitation assay (RIPA) buffer and precleared. Immunoprecipitation was performed with 5 g of rabbit anti--catenin (S ...
... homogenized into a single-cell suspension. Nuclei from crosslinked cells were resuspended in Tris-EDTA buffer and sonicated. The soluble chromatin was transferred into radioimmunoprecipitation assay (RIPA) buffer and precleared. Immunoprecipitation was performed with 5 g of rabbit anti--catenin (S ...
Centrosome misorientation mediates slowing of the cell cycle under
... Revised: Jan 30, 2012 Accepted: Feb 17, 2012 ...
... Revised: Jan 30, 2012 Accepted: Feb 17, 2012 ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.