The role of cytoplasmic streaming in symplastic transport
... & Srivastava 1950 and van Kampen 1981). Diffusion can move metabolites and messengers perpendicularly to the streamlines of cytoplasmic flow. 3 Tow. This denotes a motive force imposed differentially upon certain classes of entities in a medium. For example, the motion of electrons and holes in a se ...
... & Srivastava 1950 and van Kampen 1981). Diffusion can move metabolites and messengers perpendicularly to the streamlines of cytoplasmic flow. 3 Tow. This denotes a motive force imposed differentially upon certain classes of entities in a medium. For example, the motion of electrons and holes in a se ...
Covalent interactions are not required to permit or stabilize the non
... study to express core, E1 and E2 from both H77 and Glasgow strains are given in Fig. 1. Cloned cDNA fragments from HCV strain Glasgow, which has a 1a genotype, were kindly provided by M. McElwee and R. M. Elliott. A plasmid, pCV-H77C (kindly supplied by J. Bukh), carrying the infectious full-length ...
... study to express core, E1 and E2 from both H77 and Glasgow strains are given in Fig. 1. Cloned cDNA fragments from HCV strain Glasgow, which has a 1a genotype, were kindly provided by M. McElwee and R. M. Elliott. A plasmid, pCV-H77C (kindly supplied by J. Bukh), carrying the infectious full-length ...
Biofuel cells and their development - ePrints Soton
... 1791), when it was discovered that current from a static electricity generator could cause a severed frog’s leg to twitch, revolutionising the understanding of the nervous system. The fuel cell has been known for almost as long, since Grove successfully reversed the action of the electrolysis of wat ...
... 1791), when it was discovered that current from a static electricity generator could cause a severed frog’s leg to twitch, revolutionising the understanding of the nervous system. The fuel cell has been known for almost as long, since Grove successfully reversed the action of the electrolysis of wat ...
A cell wall reference profile for Miscanthus bioenergy crops
... solutions, but also to improve our fundamental understanding of the cell wall. Previous work based on Fourier transform midinfrared spectroscopy (FTIR) studies suggested that structural polysaccharides are major contributors to compositional variability during stem development and between organs (da ...
... solutions, but also to improve our fundamental understanding of the cell wall. Previous work based on Fourier transform midinfrared spectroscopy (FTIR) studies suggested that structural polysaccharides are major contributors to compositional variability during stem development and between organs (da ...
PDF
... fully compacted. Higher concentrations had irreversible effects within the first 6 h of culture (Table 3). Ultrastructural damage to the SER was detectable within 4 h of treatment (Fig. 6) but could be overcome if cholesterol or serum cholesterol (as FCS) was added to the culture within this period. ...
... fully compacted. Higher concentrations had irreversible effects within the first 6 h of culture (Table 3). Ultrastructural damage to the SER was detectable within 4 h of treatment (Fig. 6) but could be overcome if cholesterol or serum cholesterol (as FCS) was added to the culture within this period. ...
Studies of the cat`s medial interlaminar nucleus
... of 15 normal adult cats with electrophysiological and anatomical techniques. Autoradiographs processed from cats t h a t had one eye injected with tritiated fucose and proline indicate that MIN is composed of at least two laminae, one for each eye. The area which receives input from the ipsilateral ...
... of 15 normal adult cats with electrophysiological and anatomical techniques. Autoradiographs processed from cats t h a t had one eye injected with tritiated fucose and proline indicate that MIN is composed of at least two laminae, one for each eye. The area which receives input from the ipsilateral ...
Decreased Lysosomal Dipeptidyl Aminopeptidase I Fibroblasts
... metabolism. Studies of cultured fibroblasts from DMD patients have been initiated only very recently, however, and the results reported thus far indicate that the growth characteristics and longevity in culture of these cells is normal (18), suggesting that study of these cells is not subject to the ...
... metabolism. Studies of cultured fibroblasts from DMD patients have been initiated only very recently, however, and the results reported thus far indicate that the growth characteristics and longevity in culture of these cells is normal (18), suggesting that study of these cells is not subject to the ...
Covalent interactions are not required to permit or stabilize the non
... study to express core, E1 and E2 from both H77 and Glasgow strains are given in Fig. 1. Cloned cDNA fragments from HCV strain Glasgow, which has a 1a genotype, were kindly provided by M. McElwee and R. M. Elliott. A plasmid, pCV-H77C (kindly supplied by J. Bukh), carrying the infectious full-length ...
... study to express core, E1 and E2 from both H77 and Glasgow strains are given in Fig. 1. Cloned cDNA fragments from HCV strain Glasgow, which has a 1a genotype, were kindly provided by M. McElwee and R. M. Elliott. A plasmid, pCV-H77C (kindly supplied by J. Bukh), carrying the infectious full-length ...
Bacterial Filament Systems: Toward Understanding Their Emergent
... promotes ATP hydrolysis by MinD, causing it to detach from the membrane. Together, these activities cause all three components to oscillate between cell poles, creating a time-averaged minimum of MinC at mid-cell (53). Reconstitutions of MinCDE and FtsZ in three-dimensional cell-shaped compartments ...
... promotes ATP hydrolysis by MinD, causing it to detach from the membrane. Together, these activities cause all three components to oscillate between cell poles, creating a time-averaged minimum of MinC at mid-cell (53). Reconstitutions of MinCDE and FtsZ in three-dimensional cell-shaped compartments ...
Lymphadenosis Benigna Cutis or Cutaneous Lymphoid Hyperplasia
... proliferation of neoplastic centrocytes and centroblasts.[13] Follicle compostion in follicular lymphoma is neoplastic, monomorphous and clonal, whereas in cutaneous lymphoid hyperplasia it is benign, polymorphous and polyclonal.[14] Other IHC markers which can be applied to these cases can be CD19+ ...
... proliferation of neoplastic centrocytes and centroblasts.[13] Follicle compostion in follicular lymphoma is neoplastic, monomorphous and clonal, whereas in cutaneous lymphoid hyperplasia it is benign, polymorphous and polyclonal.[14] Other IHC markers which can be applied to these cases can be CD19+ ...
ER and vacuoles: never been closer
... tonoplast intrinsic protein α-TIP, unlike the soluble vacuolar protein phytohemagglutinin (PHA), can reach the lytic vacuole even after brefeldin A (BFA) or monensin treatment when transiently expressed in tobacco leaves, suggesting the presence of different vacuolar transport routes. Few years late ...
... tonoplast intrinsic protein α-TIP, unlike the soluble vacuolar protein phytohemagglutinin (PHA), can reach the lytic vacuole even after brefeldin A (BFA) or monensin treatment when transiently expressed in tobacco leaves, suggesting the presence of different vacuolar transport routes. Few years late ...
The Living World - Chapter 4 - McGraw Hill Higher Education
... Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
... Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
Max and inhibitory c-Myc mutants induce erythroid di
... Max-Max homodimer existed at low levels under these conditions. Addition of increasing amounts In373 (Figure 1b, lanes 6 to 9) resulted in diminishing intensity of shifted bands corresponding to tMyc/Max, Max/Max and tMyc/tMyc. These results indicate that recombinant In373 protein is able to disrupt ...
... Max-Max homodimer existed at low levels under these conditions. Addition of increasing amounts In373 (Figure 1b, lanes 6 to 9) resulted in diminishing intensity of shifted bands corresponding to tMyc/Max, Max/Max and tMyc/tMyc. These results indicate that recombinant In373 protein is able to disrupt ...
Tracing Hematopoietic Precursor Migration to Successive
... extended the analysis to two other early acting hematopoietic transcription factors, runx1 and ikaros (Figure 2; Gering and Patient, 2005). At 26 and 35 hpf, c-myb was expressed all along the trunk in a cell cord between the closely joined DA and PCV (DA-PCV joint), from somite 6 to the cloaca (somi ...
... extended the analysis to two other early acting hematopoietic transcription factors, runx1 and ikaros (Figure 2; Gering and Patient, 2005). At 26 and 35 hpf, c-myb was expressed all along the trunk in a cell cord between the closely joined DA and PCV (DA-PCV joint), from somite 6 to the cloaca (somi ...
Physiological Structure and Single
... of this concept from a sanitary and clinical viewpoint is the realization that culturability cannot be used as the ultimate indicator of cellular viability or survival. Kell et al. (1998) point out that dormancy refers to cells that have negligible activity but that are ultimately culturable, wherea ...
... of this concept from a sanitary and clinical viewpoint is the realization that culturability cannot be used as the ultimate indicator of cellular viability or survival. Kell et al. (1998) point out that dormancy refers to cells that have negligible activity but that are ultimately culturable, wherea ...
Steven Lindow - Biocontrol 2016
... Hemagglutinin adhesins Extracellular polysaccharides (EPS): gum Type I pili (anchoring) ...
... Hemagglutinin adhesins Extracellular polysaccharides (EPS): gum Type I pili (anchoring) ...
spp. Listeria Freeze-Thaw Tolerance of Role of Growth
... Pronounced cryotolerance was exhibited by cells grown at 37°C, with a <1-log decrease after 18 cycles of freezing and thawing. In contrast, freeze-thaw tolerance was significantly reduced (P < 0.05) when bacteria were grown at either 4 or 25°C, with log decreases after 18 freeze-thaw cycles ranging ...
... Pronounced cryotolerance was exhibited by cells grown at 37°C, with a <1-log decrease after 18 cycles of freezing and thawing. In contrast, freeze-thaw tolerance was significantly reduced (P < 0.05) when bacteria were grown at either 4 or 25°C, with log decreases after 18 freeze-thaw cycles ranging ...
(6R)-hydroxy-BFA in tobacco and Arabidopsis
... cytostatic activity against many cancer cell lines (Betina et al., 1962). However, because of its poor availability and poor pharmacological properties, BFA has not established itself as an anti-cancer drug. Thus, several analogues have been synthesized in order to improve solubility (Anadu et al., ...
... cytostatic activity against many cancer cell lines (Betina et al., 1962). However, because of its poor availability and poor pharmacological properties, BFA has not established itself as an anti-cancer drug. Thus, several analogues have been synthesized in order to improve solubility (Anadu et al., ...
Supplement
... SOPs selected second, third and fourth in each cluster. Let s2 be the selection time of the second SOP, s3 be the selection time for the third and s4 for the fourth (see Supporting Table 1, s2 corresponds to R2 and L2, s3 to R3 and L3 etc.). We first computed the average value of the differences bet ...
... SOPs selected second, third and fourth in each cluster. Let s2 be the selection time of the second SOP, s3 be the selection time for the third and s4 for the fourth (see Supporting Table 1, s2 corresponds to R2 and L2, s3 to R3 and L3 etc.). We first computed the average value of the differences bet ...
Phage adsorption and lytic propagation in
... As it is well known, the first step in phage infection is the adsorption on the host cell surface. This stage depends on the presence of specific attachment sites (phage receptors) on the cell wall surface [17]. In the present work, more than 99 % of phage particles were adsorbed on L. plantarum ATC ...
... As it is well known, the first step in phage infection is the adsorption on the host cell surface. This stage depends on the presence of specific attachment sites (phage receptors) on the cell wall surface [17]. In the present work, more than 99 % of phage particles were adsorbed on L. plantarum ATC ...
A molecular framework for the inhibition of
... 2004), resulting in growth retardation of the primary root. Hormones not only exert intrinsic growth control but also mediate adaptation of plant development to transiently changing environmental conditions. ABA and ethylene synthesis are induced by salt stress (Achard et al. 2006; Huang et al. 2008 ...
... 2004), resulting in growth retardation of the primary root. Hormones not only exert intrinsic growth control but also mediate adaptation of plant development to transiently changing environmental conditions. ABA and ethylene synthesis are induced by salt stress (Achard et al. 2006; Huang et al. 2008 ...
Asymmetric adhesion of rod-shaped bacteria controls microcolony
... boundaries of the colony exposed to non-kin bacteria [14, 15], coordinate cell movements [16, 17], convey signals through the colony [18] and promote the use of public good secretion [19]. However, bacterial elongation, which drives the expansion of the microcolony, competes with both cell-cell cohe ...
... boundaries of the colony exposed to non-kin bacteria [14, 15], coordinate cell movements [16, 17], convey signals through the colony [18] and promote the use of public good secretion [19]. However, bacterial elongation, which drives the expansion of the microcolony, competes with both cell-cell cohe ...
Coupled elasticity–diffusion model for the effects of cytoskeleton
... phagocytosis of filamentous Escherichia coli bacteria by macrophages. They found that complete uptake occurs only if one of the terminal bacteria filament poles enters the cell first. Abdolahad et al. [8] used an array of vertically aligned multiwalled CNTs (radius ¼ 32.5 nm) to distinguish healthy ...
... phagocytosis of filamentous Escherichia coli bacteria by macrophages. They found that complete uptake occurs only if one of the terminal bacteria filament poles enters the cell first. Abdolahad et al. [8] used an array of vertically aligned multiwalled CNTs (radius ¼ 32.5 nm) to distinguish healthy ...
Mechanism of Uptake and Retrograde Axonal Transport of
... types of membrane during exocytotic transmitter release, vesicle membrane seems to be retrieved selectively (25, 26, 38, 67). Less clear is the origin of synaptic vesicles. Vesicles, especially large dense-core vesicles in adrenergic or serotoninergic neurons, are rapidly transported from the cell b ...
... types of membrane during exocytotic transmitter release, vesicle membrane seems to be retrieved selectively (25, 26, 38, 67). Less clear is the origin of synaptic vesicles. Vesicles, especially large dense-core vesicles in adrenergic or serotoninergic neurons, are rapidly transported from the cell b ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.