Reversal of Physiological Stress
... Solid tumors often have irregular and inadequate vascularization because of the uncontrolled cellular growth associated with tumor formation. Inadequate blood flow creates cell subpopulations within tumors that are hypoxic and/or glucose-deprived (Vaupel et al., 1989). These physiological stress con ...
... Solid tumors often have irregular and inadequate vascularization because of the uncontrolled cellular growth associated with tumor formation. Inadequate blood flow creates cell subpopulations within tumors that are hypoxic and/or glucose-deprived (Vaupel et al., 1989). These physiological stress con ...
Taxonomy, biology and physiology of fungi
... F. vegetative body may be unicellular (yeasts) or multicellular moulds composed of microscopic threads called hyphae. G. cell walls composed of mostly of chitin and glucan. H. Complex cytoplasm with internal organelles, microfilaments and ...
... F. vegetative body may be unicellular (yeasts) or multicellular moulds composed of microscopic threads called hyphae. G. cell walls composed of mostly of chitin and glucan. H. Complex cytoplasm with internal organelles, microfilaments and ...
Arginase I Production in the Tumor
... San Diego, CA), in the absence of L-Arg for 24 h. The stimulated T cells were then cultured in the upper chamber of a transwell system, which has 0.4-m pores (Falcon-Becton Dickinson). The expression of CD3 in the T cells was tested after 24, 48, and 72 h. Results were expressed as mean fluorescen ...
... San Diego, CA), in the absence of L-Arg for 24 h. The stimulated T cells were then cultured in the upper chamber of a transwell system, which has 0.4-m pores (Falcon-Becton Dickinson). The expression of CD3 in the T cells was tested after 24, 48, and 72 h. Results were expressed as mean fluorescen ...
Inorganic pyrophosphate is located primarily in the mitochondria of
... demonstrated that freeze-clamped livers of rats treated with glucagon or phenylephrine show a highly significant increase in tissue PPi, from a control value of about 10 nmol/g wet wt to about 14 and 12 nmol/g wet wt. in the presence of the respective hormones. If it is assumed that all this PP1 is ...
... demonstrated that freeze-clamped livers of rats treated with glucagon or phenylephrine show a highly significant increase in tissue PPi, from a control value of about 10 nmol/g wet wt to about 14 and 12 nmol/g wet wt. in the presence of the respective hormones. If it is assumed that all this PP1 is ...
Eyes Absent, a key repressor of polar cell fate during
... Mutation of eya, like ectopic HH signaling, causes ectopic polar cells In wild-type egg chambers, the anterior polar cells recruit four to eight follicle cells to surround them and become migratory border cells at early stage 9 (Fig. 1A). They migrate through the nurse cell cluster during stage 9 (F ...
... Mutation of eya, like ectopic HH signaling, causes ectopic polar cells In wild-type egg chambers, the anterior polar cells recruit four to eight follicle cells to surround them and become migratory border cells at early stage 9 (Fig. 1A). They migrate through the nurse cell cluster during stage 9 (F ...
Annual Report 2014 The Kidney Project
... Report. This report will present Phase I and II accomplishments thus far, as well as the next steps and requirements for successful completion of the device. We also present the philanthropic support we’ve received in the past year, as these gifts have been instrumental in our success. Looking Ahead ...
... Report. This report will present Phase I and II accomplishments thus far, as well as the next steps and requirements for successful completion of the device. We also present the philanthropic support we’ve received in the past year, as these gifts have been instrumental in our success. Looking Ahead ...
Cardiac optogenetics - Biomedical Engineering
... voltage-sensitive proteins, are considered as part of the broader view of optogenetics (5, 10, 37, 95), despite the lack of an actuation component per se. Such genetically encoded sensors offer cell-specific readout and the potential for long-term in vivo monitoring of electrical activity compared w ...
... voltage-sensitive proteins, are considered as part of the broader view of optogenetics (5, 10, 37, 95), despite the lack of an actuation component per se. Such genetically encoded sensors offer cell-specific readout and the potential for long-term in vivo monitoring of electrical activity compared w ...
T cell metabolism drives immunity
... group identified mice with enhanced CD8 T cell responses to viral and tumor challenge (Okoye et al., 2015). The source of the heightened immunity gained after germline mutagenesis was the increased expression of an orphan protein, identified as lymphocyte expansion molecule (LEM). Interestingly, aug ...
... group identified mice with enhanced CD8 T cell responses to viral and tumor challenge (Okoye et al., 2015). The source of the heightened immunity gained after germline mutagenesis was the increased expression of an orphan protein, identified as lymphocyte expansion molecule (LEM). Interestingly, aug ...
4T, 3T AND 3T1D DRAM CELL DESIGN ON 32 NM
... Manufacturing process introduces variations in devices characteristic parameters, such as threshold voltage and physical dimensions (length and width) of transistors. These variations can be classified depending on their statistics as systematic (inter die and intra-die systematic) or random (intra- ...
... Manufacturing process introduces variations in devices characteristic parameters, such as threshold voltage and physical dimensions (length and width) of transistors. These variations can be classified depending on their statistics as systematic (inter die and intra-die systematic) or random (intra- ...
Taxol-stabilized Microtubules Can Position the
... at the site of furrowing (Canman et al., 2003). This led to a model in which microtubule dynamics contribute to furrow positioning, with a subpopulation of relatively stable microtubules interacting with the equatorial cortex to promote furrow formation, and dynamic microtubules at the polar regions ...
... at the site of furrowing (Canman et al., 2003). This led to a model in which microtubule dynamics contribute to furrow positioning, with a subpopulation of relatively stable microtubules interacting with the equatorial cortex to promote furrow formation, and dynamic microtubules at the polar regions ...
Full-Text PDF
... intersection of the pellicular and FP membranes as shown in Figure 1B (unpublished image). It is the structure that forms the distal region of the neck of the FP, which is also the site where the flagellum exits the FP [13,34]. Interestingly, the FPC remains attached to flagella after isolation usin ...
... intersection of the pellicular and FP membranes as shown in Figure 1B (unpublished image). It is the structure that forms the distal region of the neck of the FP, which is also the site where the flagellum exits the FP [13,34]. Interestingly, the FPC remains attached to flagella after isolation usin ...
Decreased argyrophilic nucleolar organiser region
... volume have been reported while, on the contrary, nucleolar hypertrophy has been found in Alzheimer’s disease.9–13 Nucleolar alterations, such as organisation, size and protein content changes, seem to be associated with various cellular stressors.14–16 Hypoxia, oxidants and free radicals are also k ...
... volume have been reported while, on the contrary, nucleolar hypertrophy has been found in Alzheimer’s disease.9–13 Nucleolar alterations, such as organisation, size and protein content changes, seem to be associated with various cellular stressors.14–16 Hypoxia, oxidants and free radicals are also k ...
B3 Text book - Calthorpe Park Moodle
... molecules that you need from the digested food as they pass through your small intestine. Your small intestine has several features that help you to get the most out of your food. The inner surface of the intestine is covered in small projections called villi. These greatly increase the surface area ...
... molecules that you need from the digested food as they pass through your small intestine. Your small intestine has several features that help you to get the most out of your food. The inner surface of the intestine is covered in small projections called villi. These greatly increase the surface area ...
Chapter 4
... Tissues of the body develop from three primary germ layers: Ectoderm, Endoderm, and Mesoderm Epithelial tissues develop from all three germ layers All connective tissue and most muscle tissues drive from mesoderm Nervous tissue develops from ectoderm ...
... Tissues of the body develop from three primary germ layers: Ectoderm, Endoderm, and Mesoderm Epithelial tissues develop from all three germ layers All connective tissue and most muscle tissues drive from mesoderm Nervous tissue develops from ectoderm ...
Chapter 4
... Tissues of the body develop from three primary germ layers: Ectoderm, Endoderm, and Mesoderm Epithelial tissues develop from all three germ layers All connective tissue and most muscle tissues drive from mesoderm Nervous tissue develops from ectoderm ...
... Tissues of the body develop from three primary germ layers: Ectoderm, Endoderm, and Mesoderm Epithelial tissues develop from all three germ layers All connective tissue and most muscle tissues drive from mesoderm Nervous tissue develops from ectoderm ...
Subcellular Fractionation: What You Need to Know
... Four great things about subfractionation: 1. Study single biological processes free from other interfering reactions in the cell w/o having to worry about keeping the cells alive-cell free system 2. Fractions are biologically active and can be stored 3. Minimal equipment needed and starting materia ...
... Four great things about subfractionation: 1. Study single biological processes free from other interfering reactions in the cell w/o having to worry about keeping the cells alive-cell free system 2. Fractions are biologically active and can be stored 3. Minimal equipment needed and starting materia ...
Architectural remodeling of the tonoplast during fluid
... condition scarcely observed when betaine was used as osmotic control. These vesicles are unquestionably of vacuolar origin given their red betacyanin content (Fig. 2C and D). The vacuolederived vesicles not only varied in size and number between cells from different beet hypocotyl preparations, but ...
... condition scarcely observed when betaine was used as osmotic control. These vesicles are unquestionably of vacuolar origin given their red betacyanin content (Fig. 2C and D). The vacuolederived vesicles not only varied in size and number between cells from different beet hypocotyl preparations, but ...
Endocytosis unplugged: multiple ways to enter the cell
... Endocytosis at a scale smaller than 200 nm poses specific demands on membrane machinery needed to bend membrane at this scale [41]. The eukaryotic cell appears to have resolved this in many ways. There are several modes of endocytic processes, distinguished by specific sets of molecular regulators a ...
... Endocytosis at a scale smaller than 200 nm poses specific demands on membrane machinery needed to bend membrane at this scale [41]. The eukaryotic cell appears to have resolved this in many ways. There are several modes of endocytic processes, distinguished by specific sets of molecular regulators a ...
Dynamic Interplay between Nucleoid Segregation
... 2007). However, the mechanisms governing the dynamic micromorphology of cp nucleoids remain largely unknown. A few studies examined mutants defective in cp nucleoid segregation. The monokaryotic chloroplast (moc) mutants exhibiting impaired cp nucleoid dispersion were obtained by insertional mutagen ...
... 2007). However, the mechanisms governing the dynamic micromorphology of cp nucleoids remain largely unknown. A few studies examined mutants defective in cp nucleoid segregation. The monokaryotic chloroplast (moc) mutants exhibiting impaired cp nucleoid dispersion were obtained by insertional mutagen ...
Reactive Oxygen Intermediates Activate NF-KB in a
... Tyrosine phosphorylation is an early response to stimulation via sIg in B lymphocytes* and via CD3/Ti and accessory molecules in T lymphocyte^.','^ Tyrosine phosphorylation is essential for both T-cell” and B-cell” activation, as shown by the use of tyrosine kinase inhibitors. The src family tyrosin ...
... Tyrosine phosphorylation is an early response to stimulation via sIg in B lymphocytes* and via CD3/Ti and accessory molecules in T lymphocyte^.','^ Tyrosine phosphorylation is essential for both T-cell” and B-cell” activation, as shown by the use of tyrosine kinase inhibitors. The src family tyrosin ...
Reactive Oxygen Intermediates Activate NF-KB in a
... Tyrosine phosphorylation is an early response to stimulation via sIg in B lymphocytes* and via CD3/Ti and accessory molecules in T lymphocyte^.','^ Tyrosine phosphorylation is essential for both T-cell” and B-cell” activation, as shown by the use of tyrosine kinase inhibitors. The src family tyrosin ...
... Tyrosine phosphorylation is an early response to stimulation via sIg in B lymphocytes* and via CD3/Ti and accessory molecules in T lymphocyte^.','^ Tyrosine phosphorylation is essential for both T-cell” and B-cell” activation, as shown by the use of tyrosine kinase inhibitors. The src family tyrosin ...
High Peak SAR Exposure Unit With Tight Exposure and
... following conclusions can be drawn with respect to the formulated requirements, which are: 1) the RF chamber in -polarization cannot fulfill the requirements for SAR uniformity, SAR efficiency, and small size to fit inside an incubator. 2) The RTL setup provides good performance for studies with lar ...
... following conclusions can be drawn with respect to the formulated requirements, which are: 1) the RF chamber in -polarization cannot fulfill the requirements for SAR uniformity, SAR efficiency, and small size to fit inside an incubator. 2) The RTL setup provides good performance for studies with lar ...
Effect of Nitrogen and Phosphate on the Levels of
... dilution rate which gives the maximum rate of aerobic growth before ethanol production occurs. indicates that the onset of ethanol fermentation is not induced by the accumulation of one of these intermediates. It appears more probable that the depletion of one of these glycolytic intermediates could ...
... dilution rate which gives the maximum rate of aerobic growth before ethanol production occurs. indicates that the onset of ethanol fermentation is not induced by the accumulation of one of these intermediates. It appears more probable that the depletion of one of these glycolytic intermediates could ...
Local Accumulation of Acetylcholine Receptors Is
... of the cell surface charge comes from experiments with neuraminidase, which removes negatively charged sialic acid residuesfrom the cell surface.This reducesthe magnitude of electroosmosis and should reverse the electromigration of some speciesfrom cathode-seekingto anode-seeking.The observed revers ...
... of the cell surface charge comes from experiments with neuraminidase, which removes negatively charged sialic acid residuesfrom the cell surface.This reducesthe magnitude of electroosmosis and should reverse the electromigration of some speciesfrom cathode-seekingto anode-seeking.The observed revers ...
Cytoplasmic sequestration of the tumor suppressor p53 by a heat
... HCT-8 and HCT-15 were cultured in RPMI media containing 10% FBS with the HCT-15 cells also receiving 1 mM sodium pyruvate. LS123 was cultured in ATCC-formulated Eagles’ Minimum Essential media (#30–2003). The remaining cell lines were cultured in DMEM/F12 media with 10% FBS and all cell lines were g ...
... HCT-8 and HCT-15 were cultured in RPMI media containing 10% FBS with the HCT-15 cells also receiving 1 mM sodium pyruvate. LS123 was cultured in ATCC-formulated Eagles’ Minimum Essential media (#30–2003). The remaining cell lines were cultured in DMEM/F12 media with 10% FBS and all cell lines were g ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.