Plant Vacuoles
... Jauh et al., 1998). Distinctively, the membrane of the vegetative storage vacuoles contains the aquaporin d-TIP (Jauh et al., 1998; Neuhaus and Rogers, 1998). In the endosperm of cereal grains, proteins accumulate in endoplasmic reticulum (ER)–derived organelles of vacuole-like size (see below). A f ...
... Jauh et al., 1998). Distinctively, the membrane of the vegetative storage vacuoles contains the aquaporin d-TIP (Jauh et al., 1998; Neuhaus and Rogers, 1998). In the endosperm of cereal grains, proteins accumulate in endoplasmic reticulum (ER)–derived organelles of vacuole-like size (see below). A f ...
Plant Vacuoles
... Jauh et al., 1998). Distinctively, the membrane of the vegetative storage vacuoles contains the aquaporin d-TIP (Jauh et al., 1998; Neuhaus and Rogers, 1998). In the endosperm of cereal grains, proteins accumulate in endoplasmic reticulum (ER)–derived organelles of vacuole-like size (see below). A f ...
... Jauh et al., 1998). Distinctively, the membrane of the vegetative storage vacuoles contains the aquaporin d-TIP (Jauh et al., 1998; Neuhaus and Rogers, 1998). In the endosperm of cereal grains, proteins accumulate in endoplasmic reticulum (ER)–derived organelles of vacuole-like size (see below). A f ...
November 2012 - An error occurred.
... other factors, like using other mammals as outgroups. They just swabbed a few dozen human belly buttons and looked in petri dishes for what turned up. In addition, they defined a “phylotype” arbitrarily, choosing RNA differences of 3% as diagnostic of species. ...
... other factors, like using other mammals as outgroups. They just swabbed a few dozen human belly buttons and looked in petri dishes for what turned up. In addition, they defined a “phylotype” arbitrarily, choosing RNA differences of 3% as diagnostic of species. ...
Assessment of antimicrobial compounds by microscopy techniques
... electron microscopy (TEM), scanning electron microscopy (SEM) and fluorescence microscopy. In particular, AFM can provide information about lipid domain dynamics and mechanical properties of phospholipid bilayers [26]. Fluorescent and electronic microscopies work with samples prepared in solution. I ...
... electron microscopy (TEM), scanning electron microscopy (SEM) and fluorescence microscopy. In particular, AFM can provide information about lipid domain dynamics and mechanical properties of phospholipid bilayers [26]. Fluorescent and electronic microscopies work with samples prepared in solution. I ...
The Interleukin-1 Family: Back to the Future
... Emphasis will be on the immunobiology of IL-1, its relatives and their receptors with a focus on selected cytokines (e.g., IL-33, IL-18, IL-36), pathways of negative regulation, orchestration of innate and adaptive lymphoid cells, and clinical implications. An Overview of IL-1 and IL-1R Family Membe ...
... Emphasis will be on the immunobiology of IL-1, its relatives and their receptors with a focus on selected cytokines (e.g., IL-33, IL-18, IL-36), pathways of negative regulation, orchestration of innate and adaptive lymphoid cells, and clinical implications. An Overview of IL-1 and IL-1R Family Membe ...
as a PDF
... fusion proteins (n = 33 cells, 4.1min average duration at 3.85sec intervals) revealed discrete sites of apparent microtubule initiation at the cell cortex (Fig. 1A,B). Microtubules that depolymerized to visible completion rarely showed recovery (48 of 50 events with >2min of imaging after depolymeri ...
... fusion proteins (n = 33 cells, 4.1min average duration at 3.85sec intervals) revealed discrete sites of apparent microtubule initiation at the cell cortex (Fig. 1A,B). Microtubules that depolymerized to visible completion rarely showed recovery (48 of 50 events with >2min of imaging after depolymeri ...
PDF
... differentiate to form secondary fiber cells. These fibers elongate and eventually form the bulk of lens tissue. The lens continues to grow throughout life, such that the original fibers, or oldest cells, occupy the lens center with progressively younger fiber cells found closer to the lens surface. ...
... differentiate to form secondary fiber cells. These fibers elongate and eventually form the bulk of lens tissue. The lens continues to grow throughout life, such that the original fibers, or oldest cells, occupy the lens center with progressively younger fiber cells found closer to the lens surface. ...
Arabidopsis root K - Journal of Cell Science
... al., 2003; Köhler et al., 2003; Takeda et al., 2008). In contrast to cation influx, the effect of ROS on cation efflux remains poorly studied. Hydroxyl radical (HR)-activated K+-efflux conductance has been observed alongside the activation of Ca2+ channels (Demidchik et al., 2003), but has not been ...
... al., 2003; Köhler et al., 2003; Takeda et al., 2008). In contrast to cation influx, the effect of ROS on cation efflux remains poorly studied. Hydroxyl radical (HR)-activated K+-efflux conductance has been observed alongside the activation of Ca2+ channels (Demidchik et al., 2003), but has not been ...
Full-Text PDF
... respiratory disease. Only EHV-1, however, can efficiently infect the cells of lymphoid tissues, peripheral blood mononuclear cells (PBMC), and endothelial cells (EC) of blood vessels in the central nervous system and pregnant uterus [14]. In contrast to EHV-1 infection, almost all infections with EH ...
... respiratory disease. Only EHV-1, however, can efficiently infect the cells of lymphoid tissues, peripheral blood mononuclear cells (PBMC), and endothelial cells (EC) of blood vessels in the central nervous system and pregnant uterus [14]. In contrast to EHV-1 infection, almost all infections with EH ...
Critical role of cytosolic phospholipase A2a in bronchial mucus hypersecretion in
... induction of pulmonary mucus hypersecretion and mucin expression in various animal models of asthma [15, 16] and chronic obstructive pulmonary disease [17]. Although CFTR mutations have been shown to induce mucus dehydration and accumulation, the impact of these mutations on mucin expression is stil ...
... induction of pulmonary mucus hypersecretion and mucin expression in various animal models of asthma [15, 16] and chronic obstructive pulmonary disease [17]. Although CFTR mutations have been shown to induce mucus dehydration and accumulation, the impact of these mutations on mucin expression is stil ...
A1982PM90100001
... periments showed that distilled water was preferable to buffers for release of folate from red cells. As with the serum folate assay, it was necessary to add ascorbic acid to protect the folate from oxidative destruction in subsequent autoclaving. We showed that one in ten dilution of whole blàod in ...
... periments showed that distilled water was preferable to buffers for release of folate from red cells. As with the serum folate assay, it was necessary to add ascorbic acid to protect the folate from oxidative destruction in subsequent autoclaving. We showed that one in ten dilution of whole blàod in ...
Antigen Export during Liver Infection of the Malaria - edoc
... CFSElow CD45.2⫹ CD8⫹ cells in these organs (Fig. 3C). In both organs, CD8⫹ T cells isolated from mice immunized with expOVA parasites showed the highest proliferation. A substantial proportion (~10 to 25%) of CD45.2⫹ CD8⫹ T cells recruited to the liver proliferated upon infection with OVA sporozoite ...
... CFSElow CD45.2⫹ CD8⫹ cells in these organs (Fig. 3C). In both organs, CD8⫹ T cells isolated from mice immunized with expOVA parasites showed the highest proliferation. A substantial proportion (~10 to 25%) of CD45.2⫹ CD8⫹ T cells recruited to the liver proliferated upon infection with OVA sporozoite ...
The homeobox-containing gene Wariai regulates anterior
... specific developmental stage, the organisms were fixed briefly in Z buffer with 0.5% glutaraldehyde and 0.05% Triton X-100, then stained in Z buffer containing 2.5 mM K3Fe(CN)6, 2.5 mM K4Fe(CN)6 and 1 mM X-gal (5-bromo-4-chloro-3-indolyl-β-Dgalactopyranoside). For β-galactosidase and β-glucuronidase ...
... specific developmental stage, the organisms were fixed briefly in Z buffer with 0.5% glutaraldehyde and 0.05% Triton X-100, then stained in Z buffer containing 2.5 mM K3Fe(CN)6, 2.5 mM K4Fe(CN)6 and 1 mM X-gal (5-bromo-4-chloro-3-indolyl-β-Dgalactopyranoside). For β-galactosidase and β-glucuronidase ...
Only a subset of the binary cell fate decisions
... The numbSW allele was isolated through an F1 revertant screen of the P-element-induced numb1 allele. Dysgenic males of the genotype numb1 /CyO; Dr ∆ 2-3/+ were crossed to numb2 pr cn Elp/CyO females. The F1 Cy+ progeny were collected and examined for abnormal sensory bristle phenotypes on the fly no ...
... The numbSW allele was isolated through an F1 revertant screen of the P-element-induced numb1 allele. Dysgenic males of the genotype numb1 /CyO; Dr ∆ 2-3/+ were crossed to numb2 pr cn Elp/CyO females. The F1 Cy+ progeny were collected and examined for abnormal sensory bristle phenotypes on the fly no ...
NEK1 Facilitates Cohesin Removal during Mammalian
... spatial framework is potentially lethal to the cell and to the organism, since such defects can lead to aberrant chromosome segregation (known as non-disjunction) and consequent production of aneuploid gametes. Thus, it is not surprising that both synapsis and recombination are strictly controlled a ...
... spatial framework is potentially lethal to the cell and to the organism, since such defects can lead to aberrant chromosome segregation (known as non-disjunction) and consequent production of aneuploid gametes. Thus, it is not surprising that both synapsis and recombination are strictly controlled a ...
Dynamic Tubular Vacuoles Radiate Through the
... (tonoplast), which sits passively in the centre of the cell. This central vacuole performs multiple functions, and is important not only for the generation of turgor pressure but also as a store of ions, metabolites and pigments, and as a site of detoxification (Marty 1999). Recent research into vacu ...
... (tonoplast), which sits passively in the centre of the cell. This central vacuole performs multiple functions, and is important not only for the generation of turgor pressure but also as a store of ions, metabolites and pigments, and as a site of detoxification (Marty 1999). Recent research into vacu ...
basement membrane
... epithelial cells. 8. Using a light microscope, you examine a tissue and see a simple squamous epithelium on the outer surface. Can this be a sample of skin surface? 9. Name the two primary types of glandular epithelia. ...
... epithelial cells. 8. Using a light microscope, you examine a tissue and see a simple squamous epithelium on the outer surface. Can this be a sample of skin surface? 9. Name the two primary types of glandular epithelia. ...
WHRHS BIOLOGY K PROFICIENCIES
... 46. Explain how Watson and Crick derived the DNA model. Discuss the importance of polymers to life. 47. Describe DNA replication. 48. Describe the 3 types of RNA and state function of each. 49. Compare and contrast DNA and RNA. 50. Explain how the order of nucleotides in DNA codes for different amin ...
... 46. Explain how Watson and Crick derived the DNA model. Discuss the importance of polymers to life. 47. Describe DNA replication. 48. Describe the 3 types of RNA and state function of each. 49. Compare and contrast DNA and RNA. 50. Explain how the order of nucleotides in DNA codes for different amin ...
Targeted expression of SV40 T antigen in the hair follicle of
... To further investigate the abnormal ultrastructure of the cortical cells of the transgenic hairs amino acid analyses were carried out on transgenic hair samples. Table 1 shows the amino acid composition of hair from 2 mice of the no. 40 line, a severe phenotype Fo mouse (Fo-127) and a K2.10 hair-los ...
... To further investigate the abnormal ultrastructure of the cortical cells of the transgenic hairs amino acid analyses were carried out on transgenic hair samples. Table 1 shows the amino acid composition of hair from 2 mice of the no. 40 line, a severe phenotype Fo mouse (Fo-127) and a K2.10 hair-los ...
How to make spinal motor neurons - Development
... motor neuron disease and spinal cord injury has motivated attempts to utilize stem cell and reprogramming technologies to produce motor neurons for translational applications, including the modeling of these conditions. In this Primer, we will first review the processes and events that control the s ...
... motor neuron disease and spinal cord injury has motivated attempts to utilize stem cell and reprogramming technologies to produce motor neurons for translational applications, including the modeling of these conditions. In this Primer, we will first review the processes and events that control the s ...
Size, shape, and arrangement of native cellulose fibrils in maize cell
... contain only cellulose. The microfibril as a morphological unit often observed by microscopy may contain a single CEF or a small macrofibril, in both cases associated with hemicelluloses (Ding and Himmel 2006). Cellulose has been investigated for more than a 100 years. Most investigations of cellulo ...
... contain only cellulose. The microfibril as a morphological unit often observed by microscopy may contain a single CEF or a small macrofibril, in both cases associated with hemicelluloses (Ding and Himmel 2006). Cellulose has been investigated for more than a 100 years. Most investigations of cellulo ...
Comparison of Properties of Medial Entorhinal Cortex
... square current pulse with various amplitudes from a baseline potential of 260 mV (Fig 2 A and B). This current injection was tested multiple times by increasing the amplitude by steps of either 30 or 100 pA. To address the difference in adaptation in cells from different locations, we performed diff ...
... square current pulse with various amplitudes from a baseline potential of 260 mV (Fig 2 A and B). This current injection was tested multiple times by increasing the amplitude by steps of either 30 or 100 pA. To address the difference in adaptation in cells from different locations, we performed diff ...
Symbiotic Nitrogen Fixation
... limited number of host plants. However, some rhizobia, for example, Rhizobium NGR234, have a more promiscuous nature. This Rhizobium, which can nodulate various tropical legumes, excretes 18 different Nod factors (Price et al., 1992). The production of this variety of factors is thought to be the ba ...
... limited number of host plants. However, some rhizobia, for example, Rhizobium NGR234, have a more promiscuous nature. This Rhizobium, which can nodulate various tropical legumes, excretes 18 different Nod factors (Price et al., 1992). The production of this variety of factors is thought to be the ba ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.