![CURRICULUM VITAE](http://s1.studyres.com/store/data/015578309_1-528ceaee59750825c2cf0fd622587683-300x300.png)
CURRICULUM VITAE
... (round table). In: Cells of the hepatic sinusoid. A. Kirn, DL Knook, E Wisse, eds. Proceedings of the International Kupffer cell symposium held in Strasbourg, France, 1985,363,364. 16. Keller F, Pereira CA, Wild MT, Koehren F, Kirn A. - Kupffer cells activated in vitro display antiviral activity. In ...
... (round table). In: Cells of the hepatic sinusoid. A. Kirn, DL Knook, E Wisse, eds. Proceedings of the International Kupffer cell symposium held in Strasbourg, France, 1985,363,364. 16. Keller F, Pereira CA, Wild MT, Koehren F, Kirn A. - Kupffer cells activated in vitro display antiviral activity. In ...
Depletion of Calcium from the Lumen of Endoplasmic Reticulum
... nucleoplasmin (Figs. 1 and 2). After 30 min of incubation, the nuclear/cytoplasmic concentration ratio of the injected protein was '~4.3 (Fig. 2). In the presence of normal calcium-containing medium, a similar accumulation of nucleoplasmin was obtained, yielding a transport index of 4.7. The amount ...
... nucleoplasmin (Figs. 1 and 2). After 30 min of incubation, the nuclear/cytoplasmic concentration ratio of the injected protein was '~4.3 (Fig. 2). In the presence of normal calcium-containing medium, a similar accumulation of nucleoplasmin was obtained, yielding a transport index of 4.7. The amount ...
Thuy`s Rho family GTPases review
... Microtubule: critical role in mitosis and vesicle transports ...
... Microtubule: critical role in mitosis and vesicle transports ...
the peroxisomal endomembrane system and the role of the ER
... large pleomorphic structures reminiscent of the mammalian peroxisomal reticulum (Mullen et al., 2006) and are engaged in ER-destined retrograde vesicular flow (Fig. 1). Evidence for this latter conclusion comes from observations that when the tomato bushy stunt virus (TBSV) replication protein p33 i ...
... large pleomorphic structures reminiscent of the mammalian peroxisomal reticulum (Mullen et al., 2006) and are engaged in ER-destined retrograde vesicular flow (Fig. 1). Evidence for this latter conclusion comes from observations that when the tomato bushy stunt virus (TBSV) replication protein p33 i ...
StudyGuideforBI234Exam2
... 2. Know how organisms can be classified based on their oxygen requirements, and why some organisms can tolerate toxic forms of oxygen while others cannot. 3. Discuss the relationship between nitrogen in the atmosphere (N2), usable nitrogen (NH3), and nitrogen fixing bacteria 4. Know what nitrogen is ...
... 2. Know how organisms can be classified based on their oxygen requirements, and why some organisms can tolerate toxic forms of oxygen while others cannot. 3. Discuss the relationship between nitrogen in the atmosphere (N2), usable nitrogen (NH3), and nitrogen fixing bacteria 4. Know what nitrogen is ...
Interaction of rat alveolar macrophages with dental composite dust
... American Type Culture Collection (ATCC) in Ham’s F12 K medium, supplemented with 100 U penicillin, 10 μg/ ml streptomycin (Gibco) and 15% fetal calf serum (PAA, Cölbe, Germany) and kept under cell culture conditions (37°C, 5% CO2) [13, 14]. For testing purposes, macrophages were mechanically harvest ...
... American Type Culture Collection (ATCC) in Ham’s F12 K medium, supplemented with 100 U penicillin, 10 μg/ ml streptomycin (Gibco) and 15% fetal calf serum (PAA, Cölbe, Germany) and kept under cell culture conditions (37°C, 5% CO2) [13, 14]. For testing purposes, macrophages were mechanically harvest ...
A role for the Drosophila Bag-of-marbles protein in
... protein carried a 10-residue poly(His) tag that was used for Ni2+column affinity purification of the fusion protein according to manufacturers instructions (Novagen). The PmlI-SacII fragment from a near full-length bam cDNA clone was ligated as a blunt-ended fragment into BamHI site of the pET16b po ...
... protein carried a 10-residue poly(His) tag that was used for Ni2+column affinity purification of the fusion protein according to manufacturers instructions (Novagen). The PmlI-SacII fragment from a near full-length bam cDNA clone was ligated as a blunt-ended fragment into BamHI site of the pET16b po ...
characterisation of amino acid transport in red blood cells of a
... H 2 O with [14C]sucrose as extracellular space marker, was 68.6% (v/v). Amino acid transport Uptake of [ C]-labelled amino acid (10°C) was initiated by mixing cell suspension at 10°C with an equal volume of incubation medium at the same temperature containing the appropriate concentration of radioac ...
... H 2 O with [14C]sucrose as extracellular space marker, was 68.6% (v/v). Amino acid transport Uptake of [ C]-labelled amino acid (10°C) was initiated by mixing cell suspension at 10°C with an equal volume of incubation medium at the same temperature containing the appropriate concentration of radioac ...
Salmonella must be viable in order to attach to the
... Measurement of the attachment of Salmonella serotype Typhimurium to potato CWM by flow cytometry Flow cytometry was used to enumerate the number of live or dead Salm. Typhimurium JH3016 attached to potato CWM. Frozen stock CWM was defrosted and washed with PBS through a 30-lm nylon mesh filter using ...
... Measurement of the attachment of Salmonella serotype Typhimurium to potato CWM by flow cytometry Flow cytometry was used to enumerate the number of live or dead Salm. Typhimurium JH3016 attached to potato CWM. Frozen stock CWM was defrosted and washed with PBS through a 30-lm nylon mesh filter using ...
Phloem imaging - Oxford Academic
... microscopic technique that allows live-cell imaging to date. Although it is possible non-invasively to take images of cells in deeper tissue layers with CLSM, the phloem is, nevertheless, too deep inside almost all plant organs to obtain highresolution images of its subcellular structure. The first ...
... microscopic technique that allows live-cell imaging to date. Although it is possible non-invasively to take images of cells in deeper tissue layers with CLSM, the phloem is, nevertheless, too deep inside almost all plant organs to obtain highresolution images of its subcellular structure. The first ...
Regulatory assembly of the vacuolar proton pump VOV1
... The FLIM-FRET data presented here demonstrate the applicability of time resolved imaging of living yeast cells to monitor the variable subunit composition of VOV1-ATPase parts. VOV1-ATPase shows a remarkable dis- and re-assembling process in response to nutrition conditions. In order to gain detaile ...
... The FLIM-FRET data presented here demonstrate the applicability of time resolved imaging of living yeast cells to monitor the variable subunit composition of VOV1-ATPase parts. VOV1-ATPase shows a remarkable dis- and re-assembling process in response to nutrition conditions. In order to gain detaile ...
chapt 4
... If the lysosome breaks open, these enzymes will inactivate and will not damage the cell. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
... If the lysosome breaks open, these enzymes will inactivate and will not damage the cell. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
G1 Phase-Dependent Expression of Bcl
... Downloaded from molpharm.aspetjournals.org at ASPET Journals on June 15, 2017 ...
... Downloaded from molpharm.aspetjournals.org at ASPET Journals on June 15, 2017 ...
A Late Mitotic Regulatory Network Controlling
... 4-kb genomic CDC15 fragment. pJC29 (pRS426-HACDC5) was created by inserting the HA epitope into an NcoI–EcoRI site generated at the start codon of CDC5. The construct contains 300 bp of 59 sequence and 500 bp of 39 sequence in addition to the CDC5 open reading frame. pPD.2 (pRS426-CDC14HA3) contains ...
... 4-kb genomic CDC15 fragment. pJC29 (pRS426-HACDC5) was created by inserting the HA epitope into an NcoI–EcoRI site generated at the start codon of CDC5. The construct contains 300 bp of 59 sequence and 500 bp of 39 sequence in addition to the CDC5 open reading frame. pPD.2 (pRS426-CDC14HA3) contains ...
Connective Tissue4_Cartilage and Bone
... 2. Osteocyte is the resting cell (renews matrix) that becomes trapped within its own matrix 3. Osteoclast a. Multinucleated giant cell b. sits in the resorbing canal and on the surface of trabeculae c. has a Ruffled border and contains vacuoles d. derived from monocyte/phagocyte line e. resorbs bone ...
... 2. Osteocyte is the resting cell (renews matrix) that becomes trapped within its own matrix 3. Osteoclast a. Multinucleated giant cell b. sits in the resorbing canal and on the surface of trabeculae c. has a Ruffled border and contains vacuoles d. derived from monocyte/phagocyte line e. resorbs bone ...
PDF - Blood Journal
... dilution buffer (20 mM Tris, pH8.1; 150 mM NaCl; 2 mM EDTA; 0.01% SDS; 1% Triton X-100; 1 g/mL leupeptin; 50 g/mL PMSF) and precleared with 25 L preimmune serum followed by 50 L protein A–sepharose. The supernatant was collected and an aliquot removed (input) for subsequent PCR analysis. The rem ...
... dilution buffer (20 mM Tris, pH8.1; 150 mM NaCl; 2 mM EDTA; 0.01% SDS; 1% Triton X-100; 1 g/mL leupeptin; 50 g/mL PMSF) and precleared with 25 L preimmune serum followed by 50 L protein A–sepharose. The supernatant was collected and an aliquot removed (input) for subsequent PCR analysis. The rem ...
Direct Engagement of Inhibitory Fc Receptor Microbial Immune
... genes). Fc␥RI, Fc␥RIIA, and Fc␥RIII are activating receptors associated with ITAM (15), whereas Fc␥RIIB is an inhibitory receptor that has an ITIM in its cytoplasmic tail (16). Many immunosuppressive signals described for Fc␥RIIB such as inhibition of phagocytosis of IgG-coated particles (17), calci ...
... genes). Fc␥RI, Fc␥RIIA, and Fc␥RIII are activating receptors associated with ITAM (15), whereas Fc␥RIIB is an inhibitory receptor that has an ITIM in its cytoplasmic tail (16). Many immunosuppressive signals described for Fc␥RIIB such as inhibition of phagocytosis of IgG-coated particles (17), calci ...
- ISpatula
... expected to segregate into the core of the micelles, while the hydrophilic component forms the corona or outer shell. The hydrophobic micelle core serves as a microenvironment for incorporation of various therapeutic compounds, while the corona can act as a stabilizing interface between the hydropho ...
... expected to segregate into the core of the micelles, while the hydrophilic component forms the corona or outer shell. The hydrophobic micelle core serves as a microenvironment for incorporation of various therapeutic compounds, while the corona can act as a stabilizing interface between the hydropho ...
PPT - Altogen Biosystems
... among a host of 100+ cell line specific In Vitro Transfection Kits. The HCAEC Cell Transfection Reagent is a nanoparticle-based liposome mediated formulation, and it has been developed to provide high transfection efficiency with HCAEC. This cell line is a good host for studying the circulatory syst ...
... among a host of 100+ cell line specific In Vitro Transfection Kits. The HCAEC Cell Transfection Reagent is a nanoparticle-based liposome mediated formulation, and it has been developed to provide high transfection efficiency with HCAEC. This cell line is a good host for studying the circulatory syst ...
Massive Expansion of Antigen-Specific CD8 T Cells during an Acute
... LCMV-specific CD81 cells at the peak of the immune response to LCMV, we adoptively transferred unstimulated, LCMV-specific TCR-transgenic T cells into host mice and infected them with LCMV. Similar methods have been used to follow the in vivo behavior of both CD41 and CD81 T cells (Zimmerman et al., ...
... LCMV-specific CD81 cells at the peak of the immune response to LCMV, we adoptively transferred unstimulated, LCMV-specific TCR-transgenic T cells into host mice and infected them with LCMV. Similar methods have been used to follow the in vivo behavior of both CD41 and CD81 T cells (Zimmerman et al., ...
FAK suppresses Rho activity to promote focal adhesion turnover
... spread well, and developed a more polygonal shape with fewer focal adhesions than untransfected cells (Fig. 2). Indeed, their appearance was similar to FAK+/+ cells. Cells transfected with GFP only were undistinguishable from untransfected cells (not shown). As a further test, FAK+/+ cells were tran ...
... spread well, and developed a more polygonal shape with fewer focal adhesions than untransfected cells (Fig. 2). Indeed, their appearance was similar to FAK+/+ cells. Cells transfected with GFP only were undistinguishable from untransfected cells (not shown). As a further test, FAK+/+ cells were tran ...
Complex Receptive Fields in Primary Visual Cortex
... (Kelly and Van Essen 1974; Gilbert and Wiesel 1979; Martin and Whitteridge 1984; Hirsch and others 1998, 2002; Martinez and others 1999, 2002). Second, in vivo recordings from neurons that were intracellularly labeled demonstrated that most layer 4 simple cells project to the superficial layers of t ...
... (Kelly and Van Essen 1974; Gilbert and Wiesel 1979; Martin and Whitteridge 1984; Hirsch and others 1998, 2002; Martinez and others 1999, 2002). Second, in vivo recordings from neurons that were intracellularly labeled demonstrated that most layer 4 simple cells project to the superficial layers of t ...
The role of cytoplasmic streaming in symplastic transport
... & Srivastava 1950 and van Kampen 1981). Diffusion can move metabolites and messengers perpendicularly to the streamlines of cytoplasmic flow. 3 Tow. This denotes a motive force imposed differentially upon certain classes of entities in a medium. For example, the motion of electrons and holes in a se ...
... & Srivastava 1950 and van Kampen 1981). Diffusion can move metabolites and messengers perpendicularly to the streamlines of cytoplasmic flow. 3 Tow. This denotes a motive force imposed differentially upon certain classes of entities in a medium. For example, the motion of electrons and holes in a se ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.