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Using extracellular matrix for regenerative medicine in the spinal
Using extracellular matrix for regenerative medicine in the spinal

... tenascin and tenascineglycoprotein conjugates may either promote or inhibit axonal growth) [50]. It is important to appreciate that the ECM of embryonic, postnatal and adult spinal cord tissue is different. For example, PNN appear only late in development and are related to the increase in neural ac ...
EFFECT OF ASCORBIC ACID CONCENTRATION ON
EFFECT OF ASCORBIC ACID CONCENTRATION ON

... Ascorbic acid effect plant growth and increased activity of apical meristem. This increase in growth may due to include from increase in mitotic division in meristematic zones and cell growth in length due to ascorbic acid influence [Smirnoff et al. 2000]. Obtained results confirmed that ascorbic ac ...
Metabolism, cell growth and the bacterial cell cycle
Metabolism, cell growth and the bacterial cell cycle

... 2-fold in slow-growing E. coli K-12 cells compared with the initiation mass in cells growing at a normal rate45–47. Another appealing hypothesis is that division, rather than achievement of a specific mass, serves as a checkpoint for origin firing 48. However, replication is not notably perturbed wh ...
Facultad de Ciencias Tráfico de células dendríticas a través
Facultad de Ciencias Tráfico de células dendríticas a través

... Relatively recently, a number of molecules have been described that allowed us to specifically distinguish lymphatic vessels from blood vessels. These are: the tyrosin kinase receptor VEGFR-3 (Kaipainen et al., 1995), the membrane glycoprotein Podoplanin (Breiteneder-Geleff et al., 1999), the hyalur ...
UVA radiation causes DNA strand breaks, chromosomal
UVA radiation causes DNA strand breaks, chromosomal

... caused cell death of almost all cells except one colony that was further expanded. Different from the control HaCaT cells, nodules developed in four of the eight injection sites upon injection into nude mice. Of these, two regressed while the other two continuously expanded and were classified as inv ...
Where in the Cell Are You? Probing HIV
Where in the Cell Are You? Probing HIV

... virions traffic from infected cells to infect the adjacent cell. Although the signalling events regulating this are not yet fully elucidated, the microtubule organizing centre (MTOC) can polarize to the synapse and likely plays an important role in synapse formation [18]. Furthermore, actin polymeri ...
Msx genes define a population of mural cell - HAL
Msx genes define a population of mural cell - HAL

... 2006), together with Msx1lacZ null alleles. Using this strategy Msx1 is inactivated in the two layers of the blood vessel whereas Msx2 is inactivated only in the VSMCs. The specific αSm22Cre transgene we used was chosen to inactivate Msx2lox because of its early activation in mural cells (El-Bizri ...
Whole mount in situ hybridization shows Endo 16
Whole mount in situ hybridization shows Endo 16

... zation, the staining intensity has increased and is more uniformly distributed throughout the cytoplasm of the vegetal plate cells. Fig. 1A,B shows clearly that Endo 16 message is restricted to a ring of approximately 28 cells in 20 h embryos. The absence of staining in the center of the vegetal pla ...
Thomas Auditorium Test - Tarleton State University
Thomas Auditorium Test - Tarleton State University

... Fast block to polyspermy  Slow block to polyspermy ...
4-1
4-1

... Typically much larger than prokaryotic cells Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
Endothelial Repair and Endothelial Cell-Derived
Endothelial Repair and Endothelial Cell-Derived

... regulate in lammation, coagulation, and immune response [10]. The next controversy relates a different presentation of endothelial cell-derived MPs in plasma of healthy individuals and changing of their numbers in various CV diseases and CV risks. Interestingly, circulating number of MPs originated ...
year 12 biology - Miss Jan`s Science Wikispace
year 12 biology - Miss Jan`s Science Wikispace

... Explain what is meant by the substrate Explain how enzymes are affected by heat Explain what is meant by denature Explain how enzymes are affected by pH Explain how enzymes are affected by concentration Explain how enzymes are affected by chemicals Explain what is meant by optimum conditions with re ...
The Nimrod transmembrane receptor Eater is
The Nimrod transmembrane receptor Eater is

... and crystal cells, rounded hemocytes which contain crystals of prophenoloxidases, the enzyme zymogen of phenoloxidase that catalyses the melanization reaction against parasites or septic injury (Rizki et al., 1980; Rizki and Rizki, 1992; Lanot et al., 2001). Larval hemocytes are found in three compa ...
Stimulation of Cell Elongation by Tetraploidy in Hypocotyls of Dark
Stimulation of Cell Elongation by Tetraploidy in Hypocotyls of Dark

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... 2 or 4 h profoundly decreased protein synthesis [9]. Similarly, Mimnaugh et al. [10] have also shown that the proteasome inhibitor, lactacystin, decreased the synthesis of most cellular proteins, while it specifically induced the synthesis of stress proteins Hsp72 (heat-shock protein 72) and Hsp90 i ...
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... Exosome-like vesicles containing virulence factors, enzymes, and antigens have recently been characterized in fungal pathogens, such as Cryptococcus neoformans and Histoplasma capsulatum. Here, we describe extracellular vesicles carrying highly immunogenic ␣-linked galactopyranosyl (␣-Gal) epitopes ...
Specialized filopodia direct long-range transport of SHH
Specialized filopodia direct long-range transport of SHH

... the form of a particle that remains associated with the cell via long cytoplasmic extensions that span several cell diameters. We show that these cellular extensions are a specialized class of actin-based filopodia with novel cytoskeletal features that have not been previously described. Notably, pa ...
ANATOMY AND PHYSIOLOGY RELATED TO CLINICAL PATHOLOGY
ANATOMY AND PHYSIOLOGY RELATED TO CLINICAL PATHOLOGY

... a. Nucleus. The nucleus plays a central role because it is from this structure that information is distributed which guides the life processes of the cell. In particular, the nucleus plays a central role in cellular reproduction. Two types of structures found in the nucleus are chromosomes and nucle ...
4th quarter CRT Review
4th quarter CRT Review

... divide to produce four organisms with new combinations of genetic material, they are reproducing A. Sexually by conjugation B. Asexually by fission C. Asexually using the lytic cycle ...
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... group of Prof. Schwab at the German Cancer Research Center (DKFZ) to establish a physical map of distal 1p36 as a basis for identifying a neuroblastoma tumor suppressor gene. After receiving his diploma he moved within the DKFZ to the group of Prof. Poustka to establish a novel method for an array b ...
Dynamics of Cell Movement and Tissue Motion in Gastrulation and
Dynamics of Cell Movement and Tissue Motion in Gastrulation and

... One of the major characteristics of living organisms is the high degree of order of their constituent parts, which takes form especially during early stages of embryonic development. Embryo development in all species follows the same pattern: first the developmental axes are laid down, then cells di ...
Amniotic membrane modulates innate immune response inhibiting
Amniotic membrane modulates innate immune response inhibiting

... interferon-regulating factors-3 and -7 (IRF-3 and -7), which promote transcription of type I interferons (IFN) such as IFN-a and IFN- ...
Biology Exemplar Problems
Biology Exemplar Problems

... for classification of plants. Give hierarchy of units of classification botanists follow while classifying plants and mention different ‘Suffixes’ used for the units. ...
Pulmonary immune cells in health and disease: lymphocytes
Pulmonary immune cells in health and disease: lymphocytes

... Membrane receptors and surface markers In the last few years, it has been possible to characterize lymphocytes recovered from the BAL of healthy subjects and patients with lung disorders, by using a variety of monoclonal antibodies (MoAbs). Phenotypic data have provided a great body of information d ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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