![Microtubule](http://s1.studyres.com/store/data/014734381_1-23a257e307dcaaa82dd65fd7c22289e0-300x300.png)
Microtubule
... proliferating cell populations are subject to stringent growth control mechanisms. Cancer is an abnormal state in which uncontrolled proliferation of one or more cell populations interferes with normal biological functioning. The proliferative changes are usually accompanied by other changes in cell ...
... proliferating cell populations are subject to stringent growth control mechanisms. Cancer is an abnormal state in which uncontrolled proliferation of one or more cell populations interferes with normal biological functioning. The proliferative changes are usually accompanied by other changes in cell ...
Medicinal Chemistry of Antifungal Agents
... Fungal infections fall into two distinct categories: ...
... Fungal infections fall into two distinct categories: ...
Actin-based motility of endosomes is linked to the polar tip growth of
... to endocytic compartments in plants. To this end, the FYVE domain from the mouse Hrs protein was tandemly fused (Gillooly et al., 2000) to the C-terminus of GFP or DsRedT4, respectively. Particle bombardment was employed to transiently express the fusion proteins (GFP-FYVE; DsRed-FYVE) in onion epid ...
... to endocytic compartments in plants. To this end, the FYVE domain from the mouse Hrs protein was tandemly fused (Gillooly et al., 2000) to the C-terminus of GFP or DsRedT4, respectively. Particle bombardment was employed to transiently express the fusion proteins (GFP-FYVE; DsRed-FYVE) in onion epid ...
An improved system for competent cell preparation and
... of E. coli with plasmids. By mixing the recipient cells and plasmid DNA and spreading them directly on selective medium plates containing Ca2+, the so-called 'plate transformation' could achieve almost the same transformation efficiency as the classical transformation method with calcium, yet the wh ...
... of E. coli with plasmids. By mixing the recipient cells and plasmid DNA and spreading them directly on selective medium plates containing Ca2+, the so-called 'plate transformation' could achieve almost the same transformation efficiency as the classical transformation method with calcium, yet the wh ...
Aquaporin-Mediated Fluid Regulation in the Inner Ear
... synapses (Dallos, 1996; Slepecky, 1996). From the above it is obvious that the inner ear fluids play important roles in the process of sound perception. Firstly, fluid is the basic medium in which the mechanical sound signal travels hydrodynamically. This requires a tight volume control in order to ...
... synapses (Dallos, 1996; Slepecky, 1996). From the above it is obvious that the inner ear fluids play important roles in the process of sound perception. Firstly, fluid is the basic medium in which the mechanical sound signal travels hydrodynamically. This requires a tight volume control in order to ...
Human cloning - 10EssentialScience
... Cloning is quite inefficient and usually there are over 600 to 1000 nuclear transfers before one is able to grow into a stem cell. This inefficiency is thought to be due to geneticimprinting in the cloned adult cell that interferes with the correct gene expression in the embryo. Even those animals t ...
... Cloning is quite inefficient and usually there are over 600 to 1000 nuclear transfers before one is able to grow into a stem cell. This inefficiency is thought to be due to geneticimprinting in the cloned adult cell that interferes with the correct gene expression in the embryo. Even those animals t ...
Ty Jacobs` Bio Study Guide
... i. “hemi” meaning that the alcohol and the aldehyde or ketone are all in the same molecule. c. The reaction with the first molecule of alcohol creates an additional chiral center (the carbonyl carbon). The alcohol can add in either one of two ways, attacking either the “front” or the “back” of the c ...
... i. “hemi” meaning that the alcohol and the aldehyde or ketone are all in the same molecule. c. The reaction with the first molecule of alcohol creates an additional chiral center (the carbonyl carbon). The alcohol can add in either one of two ways, attacking either the “front” or the “back” of the c ...
The Vam6 GEF Controls TORC1 by Activating the
... (A) Loss of TORC1 (Tco89) or EGOC (Ego1, Ego3, Gtr1, and Gtr2) subunits causes a defect in recovery from rapamycin-induced growth arrest and decreases TORC1 activity. (Top) Wild-type and isogenic mutant cells were grown exponentially in YPD, treated for 6 hr with rapamycin (200 ng ml1), and then wa ...
... (A) Loss of TORC1 (Tco89) or EGOC (Ego1, Ego3, Gtr1, and Gtr2) subunits causes a defect in recovery from rapamycin-induced growth arrest and decreases TORC1 activity. (Top) Wild-type and isogenic mutant cells were grown exponentially in YPD, treated for 6 hr with rapamycin (200 ng ml1), and then wa ...
ANTIGENS IN IMMUNITY XV. ULTRASZl~IlcroRAL
... The profusion of fine processes and the relative infrequency of reticular cell bodies in any plane of section suggested that each cell emitted a large number of processes which interdigitated between lymphoid cells and with processes of lymphocytes to create an extensive dendritic web or "labyrinth" ...
... The profusion of fine processes and the relative infrequency of reticular cell bodies in any plane of section suggested that each cell emitted a large number of processes which interdigitated between lymphoid cells and with processes of lymphocytes to create an extensive dendritic web or "labyrinth" ...
Individual-based modelling of growth and migration of Salmonella
... It is the presence of inhibitory substances such as lysozyme, the shortage in available iron and the relatively high pH that make albumen an effective defence against bacteria (Baron et al., 1997). As a consequence, S. enteritidis will grow poorly or not at all in the albumen. If the initial contami ...
... It is the presence of inhibitory substances such as lysozyme, the shortage in available iron and the relatively high pH that make albumen an effective defence against bacteria (Baron et al., 1997). As a consequence, S. enteritidis will grow poorly or not at all in the albumen. If the initial contami ...
Farm dust and endotoxin protect against allergy through A20
... 2 weeks to a low dose (100 ng) of LPS or to control phosphate-buffered saline (PBS) before HDM sensitization and challenge (Fig. 1A) (see supplementary materials and methods). Sham-protected mice exhibited strong airway eosinophilia and lymphocytosis (Fig. 1B), T helper 2 (TH2)–dependent HDM allerge ...
... 2 weeks to a low dose (100 ng) of LPS or to control phosphate-buffered saline (PBS) before HDM sensitization and challenge (Fig. 1A) (see supplementary materials and methods). Sham-protected mice exhibited strong airway eosinophilia and lymphocytosis (Fig. 1B), T helper 2 (TH2)–dependent HDM allerge ...
Progressive induction of caudal neural character by graded Wnt
... that underlies the prospective caudal neural plate (Fig. 1a–f). Thus, the combined patterns of expression of Wnt8c and Wnt11 in the caudal paraxial mesoderm mimic the known distribution of tissues that possess PMC activity5. In addition, from late stage 4 onwards, caudal neural plate cells themselve ...
... that underlies the prospective caudal neural plate (Fig. 1a–f). Thus, the combined patterns of expression of Wnt8c and Wnt11 in the caudal paraxial mesoderm mimic the known distribution of tissues that possess PMC activity5. In addition, from late stage 4 onwards, caudal neural plate cells themselve ...
Differences in Whole-Cell and Single
... 1 m Zn in the nutrient solution), the Zn concentrations in the leaves of these two Thlaspi spp. varied by 10-fold (about 320 and 30 g Zn g fresh weight⫺1 in T. caerulescens and T. arvense, respectively; N. Pence, personal communication), suggesting that in vivo, the mesophyll cells from each speci ...
... 1 m Zn in the nutrient solution), the Zn concentrations in the leaves of these two Thlaspi spp. varied by 10-fold (about 320 and 30 g Zn g fresh weight⫺1 in T. caerulescens and T. arvense, respectively; N. Pence, personal communication), suggesting that in vivo, the mesophyll cells from each speci ...
Development of neural tube basal lamina during neurulation and
... secrete and deposit a complete BL. These latter observations are consistent with Fig. 6. (A) Cross section of an 8-5-day (10 somite) embryo at a level equivalent to the 15th somite (light micrograph). Scale bar, 40 jum. (B) TEM enlargement of box a in 6A; right neural fold showing the neural epithel ...
... secrete and deposit a complete BL. These latter observations are consistent with Fig. 6. (A) Cross section of an 8-5-day (10 somite) embryo at a level equivalent to the 15th somite (light micrograph). Scale bar, 40 jum. (B) TEM enlargement of box a in 6A; right neural fold showing the neural epithel ...
The Notch-target gene hairy2a impedes the involution of
... we proposed that the early organiser indeed contains cells that have the potential to develop either as notochord or FP, but the question of whether they constitute a mixed population or occupy different compartments within the organiser remained unanswered. In addition, we described that Notch sign ...
... we proposed that the early organiser indeed contains cells that have the potential to develop either as notochord or FP, but the question of whether they constitute a mixed population or occupy different compartments within the organiser remained unanswered. In addition, we described that Notch sign ...
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... secrete and deposit a complete BL. These latter observations are consistent with Fig. 6. (A) Cross section of an 8-5-day (10 somite) embryo at a level equivalent to the 15th somite (light micrograph). Scale bar, 40 jum. (B) TEM enlargement of box a in 6A; right neural fold showing the neural epithel ...
... secrete and deposit a complete BL. These latter observations are consistent with Fig. 6. (A) Cross section of an 8-5-day (10 somite) embryo at a level equivalent to the 15th somite (light micrograph). Scale bar, 40 jum. (B) TEM enlargement of box a in 6A; right neural fold showing the neural epithel ...
Products > Hep-3B Transfection Reagent
... Altogen Biosystems offers the Hep-3B Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The Hep-3B Transfection Reagent is a nanoparticle-based liposome formulation, and it has been developed to provide high transfection efficiency with the Hep-3B cell line. Thi ...
... Altogen Biosystems offers the Hep-3B Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The Hep-3B Transfection Reagent is a nanoparticle-based liposome formulation, and it has been developed to provide high transfection efficiency with the Hep-3B cell line. Thi ...
Cell wall synthesis is necessary for membrane dynamics during
... 2006) as compared with the wild type (Fig. S2). Once engulfment completed and the forespore had separated from the mother cell, the ramoplanin signal in the forespore disappeared (data not shown) likely because it is unable to cross the lipid bilayer (Hamburger et al., 2009). Next, we took advantage ...
... 2006) as compared with the wild type (Fig. S2). Once engulfment completed and the forespore had separated from the mother cell, the ramoplanin signal in the forespore disappeared (data not shown) likely because it is unable to cross the lipid bilayer (Hamburger et al., 2009). Next, we took advantage ...
Minimally invasive determination of mRNA
... the diffusing fluorescent molecules in a non-invasive way. The submicron size of the FCS detection volume makes the method especially suited for single living cell studies. In principle, any diffusible protein can be fused to a fluorescent marker such as GFP, whose concentration can be quantified with FC ...
... the diffusing fluorescent molecules in a non-invasive way. The submicron size of the FCS detection volume makes the method especially suited for single living cell studies. In principle, any diffusible protein can be fused to a fluorescent marker such as GFP, whose concentration can be quantified with FC ...
ER storage diseases: a role for ERGIC
... peri-Golgi staining was evident by immunofluorescence microscopy. At day 4, small, bright dots became evident in 2040% of ⌬CH1 producing cells (see inset). These dots, usually one in each cell, probably represent the first morphological sign of ⌬CH1 aggregation. With time, they increased progressi ...
... peri-Golgi staining was evident by immunofluorescence microscopy. At day 4, small, bright dots became evident in 2040% of ⌬CH1 producing cells (see inset). These dots, usually one in each cell, probably represent the first morphological sign of ⌬CH1 aggregation. With time, they increased progressi ...
Ligand Maintenance of Regulatory T Cells via GITR for B Cells in
... Recently, the role of B cells in autoimmune diseases has been further studied by their depletion using anti-CD20, which targets B cells from the pre-B cell to memory stages. Plasma cells, which do not express CD20, are not eliminated. In EAE, anti-CD20 depletion of B cells before induction of EAE wi ...
... Recently, the role of B cells in autoimmune diseases has been further studied by their depletion using anti-CD20, which targets B cells from the pre-B cell to memory stages. Plasma cells, which do not express CD20, are not eliminated. In EAE, anti-CD20 depletion of B cells before induction of EAE wi ...
PPT - Altogen Biosystems
... PC-12 Transfection Kit Benefits • Pre-optimized transfection protocol for PC-12 cell line • Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing • Free of serum and protein of animal origin • Compatible with standard and reverse transfection methods (both protocol ...
... PC-12 Transfection Kit Benefits • Pre-optimized transfection protocol for PC-12 cell line • Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing • Free of serum and protein of animal origin • Compatible with standard and reverse transfection methods (both protocol ...
Практичне заняття № 1
... parenchyma mesophyll. It is ... A. folded (plicate) B. palisade C. spongy D. palisade and spongy E. folded and spongy ANSWER: A 42. It is known that rhizome and roots of Inula helenuim have cavities without distinct inner boundaries filled with essential oils. They are called… A. Lysigenous receptac ...
... parenchyma mesophyll. It is ... A. folded (plicate) B. palisade C. spongy D. palisade and spongy E. folded and spongy ANSWER: A 42. It is known that rhizome and roots of Inula helenuim have cavities without distinct inner boundaries filled with essential oils. They are called… A. Lysigenous receptac ...
Identification of the Sites of Asparagine
... or 113 to disrupt the first and third glycosylation sites. The expression levels and function of TSHRS79A and TSHRT115A stably expressed in CHO cells were indistinguishable from those of wt TSHR in CHO cells (Figs. 1 and 2; Table 1). These data strongly indicate that the former possibility is correc ...
... or 113 to disrupt the first and third glycosylation sites. The expression levels and function of TSHRS79A and TSHRT115A stably expressed in CHO cells were indistinguishable from those of wt TSHR in CHO cells (Figs. 1 and 2; Table 1). These data strongly indicate that the former possibility is correc ...
Comparison of the Leukocyte differentiation
... have characteristic large granules. Monocytes make up 3% to 6% of all leukocytes, phagocytose foreign bodies, mainly become macrophages, and mostly have segmented nuclei. Lymphocytes play a central role in immunity, make up 40% of all leukocytes, have a large nucleus to cytoplasm ratio, and are main ...
... have characteristic large granules. Monocytes make up 3% to 6% of all leukocytes, phagocytose foreign bodies, mainly become macrophages, and mostly have segmented nuclei. Lymphocytes play a central role in immunity, make up 40% of all leukocytes, have a large nucleus to cytoplasm ratio, and are main ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.