Comparison of the Leukocyte differentiation
... have characteristic large granules. Monocytes make up 3% to 6% of all leukocytes, phagocytose foreign bodies, mainly become macrophages, and mostly have segmented nuclei. Lymphocytes play a central role in immunity, make up 40% of all leukocytes, have a large nucleus to cytoplasm ratio, and are main ...
... have characteristic large granules. Monocytes make up 3% to 6% of all leukocytes, phagocytose foreign bodies, mainly become macrophages, and mostly have segmented nuclei. Lymphocytes play a central role in immunity, make up 40% of all leukocytes, have a large nucleus to cytoplasm ratio, and are main ...
Microbiology
... filament protein of the polar flagellum as the antigenic determinant for class 2 mAbs. Firstly, mAb binding to a 100 kDa protein on Western blots of a 2-D fingerprint of outer-membrane proteins. This protein was identified as the major constituent of a polar flagellum preparation by SDS-PAGE. Second ...
... filament protein of the polar flagellum as the antigenic determinant for class 2 mAbs. Firstly, mAb binding to a 100 kDa protein on Western blots of a 2-D fingerprint of outer-membrane proteins. This protein was identified as the major constituent of a polar flagellum preparation by SDS-PAGE. Second ...
The measurement of exocytosis in plant cells
... plasma membrane growth and for its differentiation during the life of the cell is incorporated from the secretory vesicle membrane during exocytosis. For each of these processes (polysaccharide, protein and phospholipid delivery) there may be alternative routes, but the available evidence suggests t ...
... plasma membrane growth and for its differentiation during the life of the cell is incorporated from the secretory vesicle membrane during exocytosis. For each of these processes (polysaccharide, protein and phospholipid delivery) there may be alternative routes, but the available evidence suggests t ...
scavenger receptor class b type i is a host entry factor - HAL
... Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Scavenger receptor class B type I (SR-BI) has been shown to bind HCV envelope glycoprotein E2, participate in entry of HCV pseudotype particles and modulate HCV infection. However, the functional role of SR-BI for productive HC ...
... Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Scavenger receptor class B type I (SR-BI) has been shown to bind HCV envelope glycoprotein E2, participate in entry of HCV pseudotype particles and modulate HCV infection. However, the functional role of SR-BI for productive HC ...
Manipulation of oxidative protein folding and PDI redox state in
... serological detection, we tagged murine J chains with the myc epitope. To synchronize disul®de bond formation and to uncouple oxidation and isomerization, JcM expressing HeLa transfectants were pulsed in the presence of dithiothreitol (DTT) (Braakman et al., 1992a; Valetti and Sitia, 1994), and then ...
... serological detection, we tagged murine J chains with the myc epitope. To synchronize disul®de bond formation and to uncouple oxidation and isomerization, JcM expressing HeLa transfectants were pulsed in the presence of dithiothreitol (DTT) (Braakman et al., 1992a; Valetti and Sitia, 1994), and then ...
Manipulation of oxidative protein folding and PDI redox state in
... serological detection, we tagged murine J chains with the myc epitope. To synchronize disul®de bond formation and to uncouple oxidation and isomerization, JcM expressing HeLa transfectants were pulsed in the presence of dithiothreitol (DTT) (Braakman et al., 1992a; Valetti and Sitia, 1994), and then ...
... serological detection, we tagged murine J chains with the myc epitope. To synchronize disul®de bond formation and to uncouple oxidation and isomerization, JcM expressing HeLa transfectants were pulsed in the presence of dithiothreitol (DTT) (Braakman et al., 1992a; Valetti and Sitia, 1994), and then ...
PPT - Altogen Biosystems
... Products > Beta-TC-6 Transfection Reagent (Pancreas Tumor, CRL-11506) Altogen Biosystems offers the Beta-TC-6 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The Beta-TC-6 Transfection Reagent is a two component lipidmediated formulation, and it has been deve ...
... Products > Beta-TC-6 Transfection Reagent (Pancreas Tumor, CRL-11506) Altogen Biosystems offers the Beta-TC-6 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The Beta-TC-6 Transfection Reagent is a two component lipidmediated formulation, and it has been deve ...
Archives of Microbiology
... allowing the microbes to scavenge nutrients during the colonization of animal hosts. However, the expression of putative virulence factors by bacterial cells exposed to hostile environments may be a strategy that microbes use to become successful commensal and pathogenic organisms. Thus, virulent de ...
... allowing the microbes to scavenge nutrients during the colonization of animal hosts. However, the expression of putative virulence factors by bacterial cells exposed to hostile environments may be a strategy that microbes use to become successful commensal and pathogenic organisms. Thus, virulent de ...
Expression and function of cell adhesion molecules during neural
... types. NC cell-cell and cell-matrix interactions are an essential part of the migration ...
... types. NC cell-cell and cell-matrix interactions are an essential part of the migration ...
At the border: the plasma membrane–cell wall continuum
... also contribute to cellulose synthesis (Xie et al., 2011; Wolf et al., 2012b). Therefore, the cellulose deficiencies observed in sterolimpaired plants could be a consequence of such metabolic pathways rather than membrane composition defects. More biochemical and cell biology data are needed to clar ...
... also contribute to cellulose synthesis (Xie et al., 2011; Wolf et al., 2012b). Therefore, the cellulose deficiencies observed in sterolimpaired plants could be a consequence of such metabolic pathways rather than membrane composition defects. More biochemical and cell biology data are needed to clar ...
At the border: the plasma membrane–cell wall
... also contribute to cellulose synthesis (Xie et al., 2011; Wolf et al., 2012b). Therefore, the cellulose deficiencies observed in sterolimpaired plants could be a consequence of such metabolic pathways rather than membrane composition defects. More biochemical and cell biology data are needed to clar ...
... also contribute to cellulose synthesis (Xie et al., 2011; Wolf et al., 2012b). Therefore, the cellulose deficiencies observed in sterolimpaired plants could be a consequence of such metabolic pathways rather than membrane composition defects. More biochemical and cell biology data are needed to clar ...
Regulation of endothelial and hematopoietic development by the
... arise. Indeed, recent lineage tracing studies using an Etv2 enhancer to direct expression of Cre recombinase demonstrated that Etv2-expressing progenitors contribute to the vasculature of the developing embryo and to hematopoietic cells including CD45þ nonerythroid and Ter119þ erythroid cells [36 ]. ...
... arise. Indeed, recent lineage tracing studies using an Etv2 enhancer to direct expression of Cre recombinase demonstrated that Etv2-expressing progenitors contribute to the vasculature of the developing embryo and to hematopoietic cells including CD45þ nonerythroid and Ter119þ erythroid cells [36 ]. ...
A Role in Migration for the v 1 Integrin Expressed on
... (Sigma) maintained at 378C to prevent setting of the agarose; 1.5 ml drops of the cell suspension were applied to the center of wells within a 24-well tissue culture dish (Nunc), which was then placed at 48C for 15 min to allow the agarose to solidify. Three different sets of substrates were used in ...
... (Sigma) maintained at 378C to prevent setting of the agarose; 1.5 ml drops of the cell suspension were applied to the center of wells within a 24-well tissue culture dish (Nunc), which was then placed at 48C for 15 min to allow the agarose to solidify. Three different sets of substrates were used in ...
Text - Enlighten - University of Glasgow
... the membrane. The problem is compounded further because, for charge-carrying transporters operating across a common membrane, these voltage dependencies are frequently non-linear in their characteristics (Blatt and Slayman, 1987; Blatt et al., 1987; Sanders, 1990; Weiss, 1996; Blatt, 2004a). Thus, t ...
... the membrane. The problem is compounded further because, for charge-carrying transporters operating across a common membrane, these voltage dependencies are frequently non-linear in their characteristics (Blatt and Slayman, 1987; Blatt et al., 1987; Sanders, 1990; Weiss, 1996; Blatt, 2004a). Thus, t ...
Galectin-3 Cleavage Alters Bone Remodeling: Different Outcomes in
... Cancer bone metastasis remains as a debilitating clinical problem in patients with prostate, breast, and other cancers that utilize either hematogenous and/or lymphatic venues. Bone colonization by disseminated cancer cells leads to communication with the host bone microenvironment. This causes bone ...
... Cancer bone metastasis remains as a debilitating clinical problem in patients with prostate, breast, and other cancers that utilize either hematogenous and/or lymphatic venues. Bone colonization by disseminated cancer cells leads to communication with the host bone microenvironment. This causes bone ...
05 Keim
... 18,23,26,37,38]. P-granules occur in many freshwater and marine uncultured bacteria [16,17], and can be induced in several cultured species by growing them in unbalanced media [14,22]. These are amorphous and are usually composed of linear chains of polyphosphate [22,35] and cations, such as magnesi ...
... 18,23,26,37,38]. P-granules occur in many freshwater and marine uncultured bacteria [16,17], and can be induced in several cultured species by growing them in unbalanced media [14,22]. These are amorphous and are usually composed of linear chains of polyphosphate [22,35] and cations, such as magnesi ...
Localization of TGN38 to the trans-Golgi Network
... both proteins was examined by immunofluorescence microscopy of fixed, permeabilized cells using an antibody to a Tac lumenal epitope (7G7) (Rubin et al., 1985a). We observed that whereas normal Tac was expressed on the plasma membrane (Fig. 1 a), the T-G-G chimera was localized to a juxtanuclear, tu ...
... both proteins was examined by immunofluorescence microscopy of fixed, permeabilized cells using an antibody to a Tac lumenal epitope (7G7) (Rubin et al., 1985a). We observed that whereas normal Tac was expressed on the plasma membrane (Fig. 1 a), the T-G-G chimera was localized to a juxtanuclear, tu ...
immunodetection of arabinogalactan proteins in different types of
... apparatus (Fig. 11, 12). Both studied AGP epitopes were detected inside the micropylar canal as well, but were not present in the somatic cells surrounding the canal. Other parts of the mature ovules were devoid of AGP epitopes, except for the funiculus vessels. ...
... apparatus (Fig. 11, 12). Both studied AGP epitopes were detected inside the micropylar canal as well, but were not present in the somatic cells surrounding the canal. Other parts of the mature ovules were devoid of AGP epitopes, except for the funiculus vessels. ...
Janice Evans
... – Fertilization / egg activation • exit from metaphase II arrest and progression into the embryonic cell cycle ...
... – Fertilization / egg activation • exit from metaphase II arrest and progression into the embryonic cell cycle ...
PDF
... Transcription factors act as molecular control of gene expression, regulating spatial, and temporal expression during various kinds of environmental responses (Nakashima et al., 2009; Wang and Wang, 2015). We identified 8976 transcription factor encoding transcripts belonging to 58 TF families, when ...
... Transcription factors act as molecular control of gene expression, regulating spatial, and temporal expression during various kinds of environmental responses (Nakashima et al., 2009; Wang and Wang, 2015). We identified 8976 transcription factor encoding transcripts belonging to 58 TF families, when ...
BCH 450 CAT 1 lectures
... oxidase-derived aldehyde residues, or with unmodified lysine residues. This results in crosslinking collagen and elastin, which is essential for stabilization of collagen fibrils and for the integrity and elasticity of mature elastin. Complex cross-links are formed in collagen called Pyridinoline wh ...
... oxidase-derived aldehyde residues, or with unmodified lysine residues. This results in crosslinking collagen and elastin, which is essential for stabilization of collagen fibrils and for the integrity and elasticity of mature elastin. Complex cross-links are formed in collagen called Pyridinoline wh ...
Origin, Early Patterning, and Fate of the Mouse Epiblast
... cells, where they form the inner cell mass (ICM). The trophectoderm overlying the ICM is called the polar trophectoderm. Interaction with the ICM is critical for polar trophectoderm cells to remain diploid and to proliferate. In contrast, cells, from the mural component of the trophectoderm, lining ...
... cells, where they form the inner cell mass (ICM). The trophectoderm overlying the ICM is called the polar trophectoderm. Interaction with the ICM is critical for polar trophectoderm cells to remain diploid and to proliferate. In contrast, cells, from the mural component of the trophectoderm, lining ...
Metabolism, cell growth and the bacterial cell cycle
... 2-fold in slow-growing E. coli K-12 cells compared with the initiation mass in cells growing at a normal rate45–47. Another appealing hypothesis is that division, rather than achievement of a specific mass, serves as a checkpoint for origin firing 48. However, replication is not notably perturbed wh ...
... 2-fold in slow-growing E. coli K-12 cells compared with the initiation mass in cells growing at a normal rate45–47. Another appealing hypothesis is that division, rather than achievement of a specific mass, serves as a checkpoint for origin firing 48. However, replication is not notably perturbed wh ...
Msx genes define a population of mural cell - HAL
... 2006), together with Msx1lacZ null alleles. Using this strategy Msx1 is inactivated in the two layers of the blood vessel whereas Msx2 is inactivated only in the VSMCs. The specific αSm22Cre transgene we used was chosen to inactivate Msx2lox because of its early activation in mural cells (El-Bizri ...
... 2006), together with Msx1lacZ null alleles. Using this strategy Msx1 is inactivated in the two layers of the blood vessel whereas Msx2 is inactivated only in the VSMCs. The specific αSm22Cre transgene we used was chosen to inactivate Msx2lox because of its early activation in mural cells (El-Bizri ...
Facultad de Ciencias Tráfico de células dendríticas a través
... Relatively recently, a number of molecules have been described that allowed us to specifically distinguish lymphatic vessels from blood vessels. These are: the tyrosin kinase receptor VEGFR-3 (Kaipainen et al., 1995), the membrane glycoprotein Podoplanin (Breiteneder-Geleff et al., 1999), the hyalur ...
... Relatively recently, a number of molecules have been described that allowed us to specifically distinguish lymphatic vessels from blood vessels. These are: the tyrosin kinase receptor VEGFR-3 (Kaipainen et al., 1995), the membrane glycoprotein Podoplanin (Breiteneder-Geleff et al., 1999), the hyalur ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.