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Pausing of Golgi Bodies on Microtubules Regulates
... images (Figures 1P and 1Q). In addition to Golgi bodies, we identified a second, relatively rare population (<1 observed per cell per 10 min imaging period) of smaller, homogeneously labeled particles with the same rapid, nonlinear movement as Golgi bodies (Figures 1J and 1K). These particles were i ...
... images (Figures 1P and 1Q). In addition to Golgi bodies, we identified a second, relatively rare population (<1 observed per cell per 10 min imaging period) of smaller, homogeneously labeled particles with the same rapid, nonlinear movement as Golgi bodies (Figures 1J and 1K). These particles were i ...
Direct interaction of FtsZ and MreB is required for septum synthesis
... proteins yield different subcellular patterns (Swulius and Jensen, 2012). Thus, the localisation pattern of MreB relative to FtsZ has not been firmly established in E. coli. Here, we report a novel role of MreB in bacterial cell division using E. coli as the model organism. Microscopic observations v ...
... proteins yield different subcellular patterns (Swulius and Jensen, 2012). Thus, the localisation pattern of MreB relative to FtsZ has not been firmly established in E. coli. Here, we report a novel role of MreB in bacterial cell division using E. coli as the model organism. Microscopic observations v ...
A TUrBO SWITCH SpEEdS Up A CRUCIAL CALCIUM PUMP
... The SERCA calcium pump is related to the PMCA calcium pump and it is present in all higher cells, where it pumps calcium from the cytoplasm into internal stores, e.g. when muscles are relaxed. This calcium pump has been extensively studied in our lab with structure determination of several high-reso ...
... The SERCA calcium pump is related to the PMCA calcium pump and it is present in all higher cells, where it pumps calcium from the cytoplasm into internal stores, e.g. when muscles are relaxed. This calcium pump has been extensively studied in our lab with structure determination of several high-reso ...
Lim, K et al Science 2015
... functions against a virus. Infected tissues often harbor an array of diverse inflammationinduced chemokines that guide effector T cell migration and retention. The predominant view in the field is that effector T cells home to the infection sites by following the prepatterned tissue-specific chemoki ...
... functions against a virus. Infected tissues often harbor an array of diverse inflammationinduced chemokines that guide effector T cell migration and retention. The predominant view in the field is that effector T cells home to the infection sites by following the prepatterned tissue-specific chemoki ...
About Basal and Squamous Cell Skin Cancer
... Most skin cancers can be prevented. The best way to lower the number of skin cancers and the pain and loss of life from this disease is to educate the public about skin cancer risk factors, prevention, and detection. It’s important for health care professionals and skin cancer survivors to remind ot ...
... Most skin cancers can be prevented. The best way to lower the number of skin cancers and the pain and loss of life from this disease is to educate the public about skin cancer risk factors, prevention, and detection. It’s important for health care professionals and skin cancer survivors to remind ot ...
Biological Classification of Mustard Plant
... salivary glands in response to the presence of food in the buccal cavity. Saliva is alkaline and contains an enzyme ptyalin. This enzyme converts starch into sugar (maltose). The morsel of food after being chewed and thoroughly mixed with the saliva is called a bolus. It is rolled down by the swallo ...
... salivary glands in response to the presence of food in the buccal cavity. Saliva is alkaline and contains an enzyme ptyalin. This enzyme converts starch into sugar (maltose). The morsel of food after being chewed and thoroughly mixed with the saliva is called a bolus. It is rolled down by the swallo ...
Thyroid from Stem cells Nature 2012
... iodide trapping and thyroid hormone synthesis. We therefore assessed day-22 cell cultures derived from the sequential Dox–rhTSH protocol for their capacity to organify iodide. The first evidence for active iodide organification was obtained by immunofluorescence detection of iodinated TG (TG-I) with ...
... iodide trapping and thyroid hormone synthesis. We therefore assessed day-22 cell cultures derived from the sequential Dox–rhTSH protocol for their capacity to organify iodide. The first evidence for active iodide organification was obtained by immunofluorescence detection of iodinated TG (TG-I) with ...
The Forces Behind Cell Movement
... blood cells) and macrophages (cells that ingest bacteria) move to the wound site to kill the microorganisms that cause infection, and fibroblasts (connective tissue cells) move there to remodel damaged structures [1]. In all these examples, cells reach their target by crawling. There are also other ...
... blood cells) and macrophages (cells that ingest bacteria) move to the wound site to kill the microorganisms that cause infection, and fibroblasts (connective tissue cells) move there to remodel damaged structures [1]. In all these examples, cells reach their target by crawling. There are also other ...
Biology State Standards 2009 Student Introduction B 6.2 If you were
... You started out as a tiny fertilized egg – a single cell. How did that single cell get to be the many cells that make you and how did those cells become all the different cells (like liver and heart cells) that perform specific functions in your body? KEY WORDS and PHRASES – differentiation; special ...
... You started out as a tiny fertilized egg – a single cell. How did that single cell get to be the many cells that make you and how did those cells become all the different cells (like liver and heart cells) that perform specific functions in your body? KEY WORDS and PHRASES – differentiation; special ...
Therapies for sarcopenia and regeneration of old skeletal muscles
... myogenesis did not significantly compromise the excellent overall in vivo myogenic capacity of even very old muscles in rodents. There is certainly evidence for subsequent disturbed progression and resolution of long-term muscle maturation after regeneration in some very old muscles but, rather than ...
... myogenesis did not significantly compromise the excellent overall in vivo myogenic capacity of even very old muscles in rodents. There is certainly evidence for subsequent disturbed progression and resolution of long-term muscle maturation after regeneration in some very old muscles but, rather than ...
Krp1, a novel kelch related protein that is involved in pseudopod
... Introduction One of the major issues of cancer progression is the complex process that results in a benign tumour becoming malignant. The ®nal change in tumours during this process is that they acquire the ability to invade, which allows them to penetrate the basement membrane and migrate into neigh ...
... Introduction One of the major issues of cancer progression is the complex process that results in a benign tumour becoming malignant. The ®nal change in tumours during this process is that they acquire the ability to invade, which allows them to penetrate the basement membrane and migrate into neigh ...
Induction of a Secondary Body Axis in Xenopus by Antibodies to
... receptors during mesoderm induction and other early patterning events (la, 13, 20b). Of particular interest in this regard is the observation that certain of the Wnt growth factors, which are homologues of the wingless segment polarity gene in Drosophila, have very potent effects on the patterning o ...
... receptors during mesoderm induction and other early patterning events (la, 13, 20b). Of particular interest in this regard is the observation that certain of the Wnt growth factors, which are homologues of the wingless segment polarity gene in Drosophila, have very potent effects on the patterning o ...
Gene Expression in Bone Cells
... (regulated on activation normal T cell expressed and secreted) and macrophage inflammatory protein 1 alpha (MIP1α) permitted formation of TRAP positive multinuclear cells in the absence of RANKL. However, these cells were negative for bone resorption. In the presence of RANKL, MCP-1 significantly in ...
... (regulated on activation normal T cell expressed and secreted) and macrophage inflammatory protein 1 alpha (MIP1α) permitted formation of TRAP positive multinuclear cells in the absence of RANKL. However, these cells were negative for bone resorption. In the presence of RANKL, MCP-1 significantly in ...
Cephalostatin 1 Selectively Triggers the Release
... Smac/DIABLO release and onset of apoptosis, suggesting that Smac/ DIABLO is required for cephalostatin 1-induced apoptosis. This new mitochondrial pathway is accompanied by marked structural changes of mitochondria as shown by transmission electron microscopy. ...
... Smac/DIABLO release and onset of apoptosis, suggesting that Smac/ DIABLO is required for cephalostatin 1-induced apoptosis. This new mitochondrial pathway is accompanied by marked structural changes of mitochondria as shown by transmission electron microscopy. ...
chapter 4 lecture with my notes
... through the mitotic division of underlying stem cells. © 2013 Pearson Education, Inc. ...
... through the mitotic division of underlying stem cells. © 2013 Pearson Education, Inc. ...
During Cytokinesis Light Chain, Tctex
... utilized to generate “bait” fusion protein constructs. Bait constructs were generated by PCR from a K46 murine B cell lymphoma cDNA library (described below); these were the N-terminal domains of Fyn (amino acids 1– 85, a mutated version with lysines 7 and 9 mutated to alanines, and truncated versio ...
... utilized to generate “bait” fusion protein constructs. Bait constructs were generated by PCR from a K46 murine B cell lymphoma cDNA library (described below); these were the N-terminal domains of Fyn (amino acids 1– 85, a mutated version with lysines 7 and 9 mutated to alanines, and truncated versio ...
The retinoblastoma homolog RBR1 mediates localization of the
... ATM and ATR is one of the first responses to DNA strand breaks and is often used as a quantitative measure of DNA damage. After immunofluorescence staining using an antibody against phosphorylated H2AX (cH2AX), we found that the nuclei of untreated rbr1 mutants had slightly more foci than the wild t ...
... ATM and ATR is one of the first responses to DNA strand breaks and is often used as a quantitative measure of DNA damage. After immunofluorescence staining using an antibody against phosphorylated H2AX (cH2AX), we found that the nuclei of untreated rbr1 mutants had slightly more foci than the wild t ...
Drosophila follicle cells are patterned by multiple
... were processed under identical conditions (Fig. 4E,F). Following expression of dskul in polar cells, Notch reporter levels were significantly elevated, both in the germarium and in stage 1-3 egg chambers. These observations indicate that Kul acts as an attenuator of Dl signaling in early-stage folli ...
... were processed under identical conditions (Fig. 4E,F). Following expression of dskul in polar cells, Notch reporter levels were significantly elevated, both in the germarium and in stage 1-3 egg chambers. These observations indicate that Kul acts as an attenuator of Dl signaling in early-stage folli ...
Distribution of polycyclic aromatic hydrocarbons in
... tissues and cells driven by transpiration and the PAH concentration gradient across plant-cell components and (2) accumulation of PAHs in plant tissues, with the extent related to plant lipid contents [18-21]. However, the factors that influence PAH transfer and distribution in plants as well as the ...
... tissues and cells driven by transpiration and the PAH concentration gradient across plant-cell components and (2) accumulation of PAHs in plant tissues, with the extent related to plant lipid contents [18-21]. However, the factors that influence PAH transfer and distribution in plants as well as the ...
Cellular damage induced by cadmium and
... sensitivity of the stress assays used, a micro-assay system, in which seedlings were exposed for 24 h, was developed. Phytotoxicity of metals, quantified as growth inhibition, was observed well before there was any change in the non-protein thiol tissue concentration. When measured with conventional ...
... sensitivity of the stress assays used, a micro-assay system, in which seedlings were exposed for 24 h, was developed. Phytotoxicity of metals, quantified as growth inhibition, was observed well before there was any change in the non-protein thiol tissue concentration. When measured with conventional ...
Latrunculin A Induced Perturbation of the Actin Cytoskeleton
... All strains used in this study were either derived from the Karagiannis lab collection, constructed during the course of this work (see Cloning Methods), or in the case of the imp1Δ and caf5Δ strains, purchased from Bioneer Corporation as part of version 4 of the haploid gene deletion mutant library ...
... All strains used in this study were either derived from the Karagiannis lab collection, constructed during the course of this work (see Cloning Methods), or in the case of the imp1Δ and caf5Δ strains, purchased from Bioneer Corporation as part of version 4 of the haploid gene deletion mutant library ...
siRNA-27 - OriGene
... control duplex for this purpose. It is a potent Dicer-Substrate duplex that has been shown to suppress HPRT mRNA levels >90% when used at 10 nM concentration. OriGene also provides a validated negative control duplex as part of the kit that is not present in the human, mouse, or rat genomes. Validat ...
... control duplex for this purpose. It is a potent Dicer-Substrate duplex that has been shown to suppress HPRT mRNA levels >90% when used at 10 nM concentration. OriGene also provides a validated negative control duplex as part of the kit that is not present in the human, mouse, or rat genomes. Validat ...
SLP-76-Cbl-Grb2-Shc Interactions in Fc RI Signaling
... conjugated to AP was purchased from Sigma (St Louis, MO). PAP was purchased from Boehringer Mannheim (Mannheim, Germany). Cells. The U937 and THP-1 cell lines were obtained from ATCC (Rockville, MD) and cultured in RPMI 1640 with 10% fetal bovine serum (FBS). U937IF cells were prepared by culturing ...
... conjugated to AP was purchased from Sigma (St Louis, MO). PAP was purchased from Boehringer Mannheim (Mannheim, Germany). Cells. The U937 and THP-1 cell lines were obtained from ATCC (Rockville, MD) and cultured in RPMI 1640 with 10% fetal bovine serum (FBS). U937IF cells were prepared by culturing ...
Example of a scientific poster - SPUR
... This work implies that all of the increase in cell proliferation in CagA flies is dependent upon the presence of the microbiota. More plating data would need to be collected in order to determine whether the Lactobacillus Drosophila bacterial group is involved in increasing cell proliferation or Ace ...
... This work implies that all of the increase in cell proliferation in CagA flies is dependent upon the presence of the microbiota. More plating data would need to be collected in order to determine whether the Lactobacillus Drosophila bacterial group is involved in increasing cell proliferation or Ace ...
A High-Resolution Transcript Profile across the
... Although all three cell types are able to undergo cell divisions, the mother cells will eventually loose their ability to divide and undergo terminal differentiation, whereas the initials retain their undifferentiated state. It is, however, almost impossible to distinguish the initials from mother c ...
... Although all three cell types are able to undergo cell divisions, the mother cells will eventually loose their ability to divide and undergo terminal differentiation, whereas the initials retain their undifferentiated state. It is, however, almost impossible to distinguish the initials from mother c ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.