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... In the present work, the specimen is observed in a specifically designed chamber, principally composed by two microscope coverslips orthogonal to the optical axis. An aluminum frame maintains the two coverslips 4 mm distant, while fixations assure the watetightness of the chamber. One lateral side ...
... In the present work, the specimen is observed in a specifically designed chamber, principally composed by two microscope coverslips orthogonal to the optical axis. An aluminum frame maintains the two coverslips 4 mm distant, while fixations assure the watetightness of the chamber. One lateral side ...
Introduction to Light Microscopy Introduction Light microscopes are
... Object detail appears in a microscope image only when the diffracted light from that detail passes into the objective lens. This is why the numerical aperture is so important in resolving power: the greater the N.A., the greater the angle at which light is accepted, and therefore, the smaller the R ...
... Object detail appears in a microscope image only when the diffracted light from that detail passes into the objective lens. This is why the numerical aperture is so important in resolving power: the greater the N.A., the greater the angle at which light is accepted, and therefore, the smaller the R ...
Non-linear Optical Microscopy and Spectroscopy for
... by Denk et al., who did experiments on fluorescent beads and on cells [5]. As excitation by two photons allows working in the near infrared (NIR) area, the technique is well suited for optically dense biological tissue [6, 7, 8, 9], such as human skin. The main advantage compared to confocal scanning ...
... by Denk et al., who did experiments on fluorescent beads and on cells [5]. As excitation by two photons allows working in the near infrared (NIR) area, the technique is well suited for optically dense biological tissue [6, 7, 8, 9], such as human skin. The main advantage compared to confocal scanning ...
Week7-animations
... laser pulses. July 5th, 2012: 192 laser beams delivered 1.85MJ and 500 trillion watts of power (short time period). 1000 times more power than used by US at any instant of time. ...
... laser pulses. July 5th, 2012: 192 laser beams delivered 1.85MJ and 500 trillion watts of power (short time period). 1000 times more power than used by US at any instant of time. ...
Partially Coherent Image Formation Theory for X
... enabled development of short wavelenth focusing elements and significantly improved the spatial resolution [4–9]. In the soft x-ray spectral region, samples as small as a few tens of nanometers can be resolved using micro zone-plates (MZP) as the objective lens [3]. In addition to conventional x-ray ...
... enabled development of short wavelenth focusing elements and significantly improved the spatial resolution [4–9]. In the soft x-ray spectral region, samples as small as a few tens of nanometers can be resolved using micro zone-plates (MZP) as the objective lens [3]. In addition to conventional x-ray ...
resolution
... A technique to get improved angular resolution using an array of telescopes. Most common in radio, but also limited optical interferometry. ...
... A technique to get improved angular resolution using an array of telescopes. Most common in radio, but also limited optical interferometry. ...
Single-pixel infrared and visible microscope
... technology is extremely well developed. Microscopy in other regions of the spectrum, such as infrared or ultraviolet, traditionally requires expensive cameras. Single-pixel imaging is, therefore, an attractive prospect as it allows the development of imaging systems that can be significantly cheaper ...
... technology is extremely well developed. Microscopy in other regions of the spectrum, such as infrared or ultraviolet, traditionally requires expensive cameras. Single-pixel imaging is, therefore, an attractive prospect as it allows the development of imaging systems that can be significantly cheaper ...
The Optical Design of Miniaturized Microscope Objective for CARS
... In recent years, non linear optical (NLO) microscopy has been highlighted for visualizing the morphological details of living tissue which cannot be resolved by ultrasound or magnetic resonance imaging. NLO microscopy utilizes the signal from two-photon excitation fluorescence (TPEF) [1-5], second h ...
... In recent years, non linear optical (NLO) microscopy has been highlighted for visualizing the morphological details of living tissue which cannot be resolved by ultrasound or magnetic resonance imaging. NLO microscopy utilizes the signal from two-photon excitation fluorescence (TPEF) [1-5], second h ...
Resolution questions with solutions
... central maximum around X; correct overall shape (only two secondary maxima need be shown); secondary maxima much less intense than the central maximum; ...
... central maximum around X; correct overall shape (only two secondary maxima need be shown); secondary maxima much less intense than the central maximum; ...
