Biology 177: Principles of Modern Microscopy
... We have looked at several different methods for optical sectioning of fluorescent samples. The two main methods are Laser Scanning Confocal Microscopy (LSCM) and light sheet microscopy or Selective Plane Illumination Microscopy (SPIM). LSCM has been around a long time compared to SPIM. Question: Do ...
... We have looked at several different methods for optical sectioning of fluorescent samples. The two main methods are Laser Scanning Confocal Microscopy (LSCM) and light sheet microscopy or Selective Plane Illumination Microscopy (SPIM). LSCM has been around a long time compared to SPIM. Question: Do ...
39 Steps
... and useful explanations are included. The terms appear in bold lettering and many interact with other terms in bold lettering. Note that many of these variables are usually thought of in terms of their effect on spatial resolution. They are listed here because reduced resolution translates into “put ...
... and useful explanations are included. The terms appear in bold lettering and many interact with other terms in bold lettering. Note that many of these variables are usually thought of in terms of their effect on spatial resolution. They are listed here because reduced resolution translates into “put ...
Microscopy Basics
... Most biological objects, however, absorb only weakly in the visible spectrum. This lead to: • Development of specific staining (nowadays almost entirely replaced by fluorescent labeling) • Development of UV microscopy (Köhler) facing technical difficulties due to absorption of UV light by glass • Us ...
... Most biological objects, however, absorb only weakly in the visible spectrum. This lead to: • Development of specific staining (nowadays almost entirely replaced by fluorescent labeling) • Development of UV microscopy (Köhler) facing technical difficulties due to absorption of UV light by glass • Us ...
High Resolution Biomedical Imaging with Light and Sound
... High resolution imaging systems are invaluable tools for biomedical research and clinical practice. Photoacoustic microscopy (PAM) is an emerging hybrid technique that can overcome the limitations of conventional optical and ultrasonic imaging modalities. A pulsed laser illuminates tissue, where opt ...
... High resolution imaging systems are invaluable tools for biomedical research and clinical practice. Photoacoustic microscopy (PAM) is an emerging hybrid technique that can overcome the limitations of conventional optical and ultrasonic imaging modalities. A pulsed laser illuminates tissue, where opt ...
Nanoscopy with focused light
... Throughout the 20th century it was widely accepted that a light microscope relying on conventional optical lenses cannot discern details that are much finer than about half the wavelength of light (200-400 nm), due to diffraction. However, in the 1990s, the viability to overcome the diffraction barr ...
... Throughout the 20th century it was widely accepted that a light microscope relying on conventional optical lenses cannot discern details that are much finer than about half the wavelength of light (200-400 nm), due to diffraction. However, in the 1990s, the viability to overcome the diffraction barr ...