Frequency Domain Optical Coherence Tomography (FDOCT)
... Abstract-An optical coherence tomography (OCT) is a noninvasive imaging technology in which an amplitude and a phase of electromagnetic wave undergone backscattering are used to extract properties and microstructure of a material medium such as biological tissues by providing a cross-sectional view ...
... Abstract-An optical coherence tomography (OCT) is a noninvasive imaging technology in which an amplitude and a phase of electromagnetic wave undergone backscattering are used to extract properties and microstructure of a material medium such as biological tissues by providing a cross-sectional view ...
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... This means that the intensity profile of an image rendered by a coherent imaging system is phase dependent. As such, simply measuring and reporting its intensity response is an unsuitable means to characterize resolution in an unambiguous manner. Further compounding the issue, many recent coherent i ...
... This means that the intensity profile of an image rendered by a coherent imaging system is phase dependent. As such, simply measuring and reporting its intensity response is an unsuitable means to characterize resolution in an unambiguous manner. Further compounding the issue, many recent coherent i ...
Superresolution size determination in fluorescence microscopy: A
... 共e.g., 650 nm兲; z i,o are the positions of the maximum of the enveloping AID profiles; C is proportional to the FWHMmod of the cos2 shaped standing wave field present between the two objective lenses; and z 2 is the position of one maximum 共arbitrarily chosen and then fixed兲 of the previously mentio ...
... 共e.g., 650 nm兲; z i,o are the positions of the maximum of the enveloping AID profiles; C is proportional to the FWHMmod of the cos2 shaped standing wave field present between the two objective lenses; and z 2 is the position of one maximum 共arbitrarily chosen and then fixed兲 of the previously mentio ...
Resolution scaling in STED microscopy
... microscope at given experimental parameters and demonstrate that this theoretical mark can indeed be reached by a standard implementation using readily available optical components. In the STED microscope the diffraction limited excitation focus is overlaid with a red shifted STED beam featuring a z ...
... microscope at given experimental parameters and demonstrate that this theoretical mark can indeed be reached by a standard implementation using readily available optical components. In the STED microscope the diffraction limited excitation focus is overlaid with a red shifted STED beam featuring a z ...
The Resolving Power Of a Microscope and Telescope
... math) can only image to about 0.1 micron. This means that usually organelles, viruses and proteins cannot be imaged. 3. Decreasing the wavelength by using X-rays and gamma rays. While these techniques are used to study inorganic crystals, biological samples are usually damaged by x-rays and hence ar ...
... math) can only image to about 0.1 micron. This means that usually organelles, viruses and proteins cannot be imaged. 3. Decreasing the wavelength by using X-rays and gamma rays. While these techniques are used to study inorganic crystals, biological samples are usually damaged by x-rays and hence ar ...
Two-Photon Excited Fluorescence Microscopy - Spectra
... sections of the illuminating cone is largest at the focal plane and dies off rapidly in the regions in front and behind it. The confinement of excitation and thus signal production in a small volume within samples is certainly the key feature of TPM. It is demonstrated in Figure 2 by comparing the e ...
... sections of the illuminating cone is largest at the focal plane and dies off rapidly in the regions in front and behind it. The confinement of excitation and thus signal production in a small volume within samples is certainly the key feature of TPM. It is demonstrated in Figure 2 by comparing the e ...
Tomographic Interference Microscopy of Living Cells
... Research on the internal structure of living cells gives important information about morphology, spatial distribution of proteins and concentration of chemical drugs inside the cell. In microscopy three types of samples are usually investigated: fluorescent or emissive samples; stained or amplitude ...
... Research on the internal structure of living cells gives important information about morphology, spatial distribution of proteins and concentration of chemical drugs inside the cell. In microscopy three types of samples are usually investigated: fluorescent or emissive samples; stained or amplitude ...
Wide-field extended-resolution fluorescence
... proposed [1]. Surface plasmon resonance (SPR) – resonant energy transfer from incident photons to electron density oscillations along a metal-dielectric interface – helps to enhance weak evanescent waves, which essentially carry the high spatial frequency information, thereby improving the imaging r ...
... proposed [1]. Surface plasmon resonance (SPR) – resonant energy transfer from incident photons to electron density oscillations along a metal-dielectric interface – helps to enhance weak evanescent waves, which essentially carry the high spatial frequency information, thereby improving the imaging r ...
report - CREATE project
... Here we demonstrate further improvement of the TPEF imaging system by introducing new excitation sourcedelivering reduced duration pulses, centered at optimized wavelength and new dispersion compensation unit, capable of compensating high order dispersion components. 2. Aim of the visit Our goal was ...
... Here we demonstrate further improvement of the TPEF imaging system by introducing new excitation sourcedelivering reduced duration pulses, centered at optimized wavelength and new dispersion compensation unit, capable of compensating high order dispersion components. 2. Aim of the visit Our goal was ...
12. confocal microscopy.
... 12.1. Confocal Microscopy Confocal microscopy can render depth-resolved slices through a 3D object by rejecting much of the out of focus light via a pinhole. In confocal microscopy the image is reconstructed serially, i.e. point by point, using a single photodetector, rather than in parallel (in bri ...
... 12.1. Confocal Microscopy Confocal microscopy can render depth-resolved slices through a 3D object by rejecting much of the out of focus light via a pinhole. In confocal microscopy the image is reconstructed serially, i.e. point by point, using a single photodetector, rather than in parallel (in bri ...