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Frequency Domain Optical Coherence Tomography (FDOCT)
Frequency Domain Optical Coherence Tomography (FDOCT)

... Abstract-An optical coherence tomography (OCT) is a noninvasive imaging technology in which an amplitude and a phase of electromagnetic wave undergone backscattering are used to extract properties and microstructure of a material medium such as biological tissues by providing a cross-sectional view ...
Biomedical imaging in the undergraduate physics curriculum
Biomedical imaging in the undergraduate physics curriculum

PDF Link
PDF Link

... This means that the intensity profile of an image rendered by a coherent imaging system is phase dependent. As such, simply measuring and reporting its intensity response is an unsuitable means to characterize resolution in an unambiguous manner. Further compounding the issue, many recent coherent i ...
Superresolution size determination in fluorescence microscopy: A
Superresolution size determination in fluorescence microscopy: A

... 共e.g., 650 nm兲; z i,o are the positions of the maximum of the enveloping AID profiles; C is proportional to the FWHMmod of the cos2 shaped standing wave field present between the two objective lenses; and z 2 is the position of one maximum 共arbitrarily chosen and then fixed兲 of the previously mentio ...
Resolution scaling in STED microscopy
Resolution scaling in STED microscopy

... microscope at given experimental parameters and demonstrate that this theoretical mark can indeed be reached by a standard implementation using readily available optical components. In the STED microscope the diffraction limited excitation focus is overlaid with a red shifted STED beam featuring a z ...
PDF
PDF

The Resolving Power Of a Microscope and Telescope
The Resolving Power Of a Microscope and Telescope

... math) can only image to about 0.1 micron. This means that usually organelles, viruses and proteins cannot be imaged. 3. Decreasing the wavelength by using X-rays and gamma rays. While these techniques are used to study inorganic crystals, biological samples are usually damaged by x-rays and hence ar ...
Viewing Microstructures of Materials using the Optical Microscope
Viewing Microstructures of Materials using the Optical Microscope

Two-Photon Excited Fluorescence Microscopy - Spectra
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... sections of the illuminating cone is largest at the focal plane and dies off rapidly in the regions in front and behind it. The confinement of excitation and thus signal production in a small volume within samples is certainly the key feature of TPM. It is demonstrated in Figure 2 by comparing the e ...
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A Brief History of the Microscope and its Significance

A guide to super-resolution fluorescence microscopy
A guide to super-resolution fluorescence microscopy

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Tomographic Interference Microscopy of Living Cells

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Diffracted Light Contrast: Improving the Resolution - Microscopy-UK

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Depth-of-Focus in Microscopy

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Wide-field extended-resolution fluorescence

... proposed [1]. Surface plasmon resonance (SPR) – resonant energy transfer from incident photons to electron density oscillations along a metal-dielectric interface – helps to enhance weak evanescent waves, which essentially carry the high spatial frequency information, thereby improving the imaging r ...
Optical, Confocal, and 4Pi Microscopy
Optical, Confocal, and 4Pi Microscopy

report - CREATE project
report - CREATE project

... Here we demonstrate further improvement of the TPEF imaging system by introducing new excitation sourcedelivering reduced duration pulses, centered at optimized wavelength and new dispersion compensation unit, capable of compensating high order dispersion components. 2. Aim of the visit Our goal was ...
12. confocal microscopy.
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... 12.1. Confocal Microscopy Confocal microscopy can render depth-resolved slices through a 3D object by rejecting much of the out of focus light via a pinhole. In confocal microscopy the image is reconstructed serially, i.e. point by point, using a single photodetector, rather than in parallel (in bri ...
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Light Microscopy [10 credits]

4Pi Microscopy
4Pi Microscopy

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PROOF COPY 069543APL
PROOF COPY 069543APL

SPDM: light microscopy with single
SPDM: light microscopy with single

1 Light Microscopy
1 Light Microscopy

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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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