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Isotropic Diffraction-Limited Focusing Using a Single Objective Lens
Isotropic Diffraction-Limited Focusing Using a Single Objective Lens

preprint version PDF - Emory Physics Department
preprint version PDF - Emory Physics Department

"Contrast Enhancement in Light Microscopy". In: Current Protocols in
"Contrast Enhancement in Light Microscopy". In: Current Protocols in

Lecture 3
Lecture 3

Jens Prescher, Establishment and Optimization of Super
Jens Prescher, Establishment and Optimization of Super

Contactless visible light probing for nanoscale ICs through 10 μm
Contactless visible light probing for nanoscale ICs through 10 μm

... low for NIR, the light reflected from the active device is relatively strong compared to the reflection from the back surface. If we now consider the case of VIS illumination as on the right side of Figure 2 we can see that due to the increased absorption the signal reflected from the active device ...
DYNAMICS OF THE CELL MEMBRANE OBSERVED UNDER THE
DYNAMICS OF THE CELL MEMBRANE OBSERVED UNDER THE

High-speed addressable confocal microscopy for functional imaging
High-speed addressable confocal microscopy for functional imaging

... back to the central optical axis, so that a stationary pinhole located in an image plane can act as a spatial filter for point detection. Despite the improvement in spatial resolution compared to traditional wide-field microscopes, there are several limitations with currently available confocal syst ...
The effect of detector size on the signal-to
The effect of detector size on the signal-to

... microscopy is to consider a signal-to-noise ratio and to select an optimum pinhole size based on maximizing this ratio (Sandison et al., 1995). We shall adapt this model to the confocal polarized light microscope. We consider a subresolution point scatterer to be located at the geometrical focal poi ...
BLUE PRINT FOR QUESTION PAPER APPLIED PHYSICS – II (R
BLUE PRINT FOR QUESTION PAPER APPLIED PHYSICS – II (R

... Introduction to nano-science and nanotechnology, Two main approaches in nanotechnology – Bottom up technique and top down technique, Tools used in nanotechnology such as scanning electron microscope, Scanning Tunneling Microscope, Atomic Force Microscope, Nanomaterials : Method to produce nano mater ...
Soft x-ray laser holography with wavelength resolution *
Soft x-ray laser holography with wavelength resolution *

Evanescent-field optical microscopy: effects of polarization, tip
Evanescent-field optical microscopy: effects of polarization, tip

repeat
repeat

... For each of the molecules shown below, CLEARLY draw an asterisk next to each stereocentre. Then indicate under each molecule whether it is CHIRAL or ACHIRAL. ...
Techniques to Improve 3D Optical Imaging
Techniques to Improve 3D Optical Imaging

the optical (light) microscope
the optical (light) microscope

... results. However, achromats do provide a relatively long working distance, that is, the distance from the front lens of the objective to the specimen surface. Working distance decreases as magnification of the objective increases. Most manufacturers make long-workingdistance objectives for special ...
Microscopy - Frank`s Hospital Workshop
Microscopy - Frank`s Hospital Workshop

... This method is of critical importance in the modern life sciences, as it can be extremely sensitive, allowing the detection of single molecules. Many different fluorescent dyes can be used to stain different structures or chemical compounds. One particularly powerful method is the combination of ant ...
setting up of a total internal reflection fluorescent microscope
setting up of a total internal reflection fluorescent microscope

... The green curve in Fig. 6b indicates the spectrum of Dichromatic beamsplitter (dichroic mirror) which is a specialized filter designed to efficiently reflect excitation wavelengths and pass emission wavelengths. These filters are always the interference type. Dichroic mirror is positioned in the lig ...
Nanometer optical coherence tomography using
Nanometer optical coherence tomography using

A1979HZ30700001
A1979HZ30700001

Lecture 1 TEM
Lecture 1 TEM

Analytical technique: Fluorescence Spectroscopy
Analytical technique: Fluorescence Spectroscopy

... be achieved either with fibre optics or by placing a sample within the spectrometer. Both excitation wavelength and emission wavelength can be varied or scanned by use of excitaiton and emission monochromators. Thus, the spectral characteristics of materials can be determined and it may be possible ...
Parallelized STED fluorescence nanoscopy
Parallelized STED fluorescence nanoscopy

... fluorescent state is inherently allowed. By precisely positioning the zero point(s) or line(s) in the sample and adjusting ISTED one can define the coordinate in space where the fluorophores can still assume the fluorescent state, i.e. are not 'switched off' by the enforced occupation of the ground ...
Microscopy 1: Optical
Microscopy 1: Optical

Synchronized ti scattering microscopy
Synchronized ti scattering microscopy

Light sheet-based fluorescence microscopy: more dimensions, more
Light sheet-based fluorescence microscopy: more dimensions, more

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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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