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MPE Tutorial Multiphoton Excitation Microscopy 5100 Patrick Henry Drive
MPE Tutorial Multiphoton Excitation Microscopy 5100 Patrick Henry Drive

Assignment #2 - Rose
Assignment #2 - Rose

Experimental studies of far-field superlens for sub-diffractional optical imaging
Experimental studies of far-field superlens for sub-diffractional optical imaging

A method for characterizing longitudinal chromatic aberration of
A method for characterizing longitudinal chromatic aberration of

Chapter 2 System Evaluation
Chapter 2 System Evaluation

... Data The NBS-1952 Resolution Test Chart is described in the : NBS circular 533, 1953 in the section titled Method of Determining the Resolution Power of Photographic Lenses. The design features of this target reduce edge effects, minimize spurious resolution and permit single pass scanning. Notes Th ...
femtosecond laser ablation of dielectrics
femtosecond laser ablation of dielectrics

... Despite the fact that the basic mechanisms of the processes have been widely studied, important questions still remain to be conclusively answered. The difficulty arises from the large number of phenomena involved during and after the interaction: non linear excitation by multiphoton absorption or t ...
Resolution [from the New Merriam-Webster Dictionary, 1989 ed.]: 3 resolve
Resolution [from the New Merriam-Webster Dictionary, 1989 ed.]: 3 resolve

High-resolution retinal microscopy using MEMS
High-resolution retinal microscopy using MEMS

... made using microelectromechanical (MEMS) systems technology has enabled rapid development of adaptive optics in several new applications. In this paper, the design and testing of a retinal imaging system is detailed, with an emphasis on the adaptive optical components and their performance. Retinal ...
Microscopy - PSSurvival.com
Microscopy - PSSurvival.com

... require an ultraviolet microscope as protein absorbs light at 280 nm. Protein will also fluorescence at approximately 353 nm when excited with 280 nm light.[4] Since fluorescence emission differs in wavelength (color) from the excitation light, an ideal fluorescent image shows only the structure of ...
Practice Problems_sources
Practice Problems_sources

CMU3 - Fast and Simple High-Resolution Optical Spectrum Analyzer
CMU3 - Fast and Simple High-Resolution Optical Spectrum Analyzer

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Principles of TEM image formation Principles of TEM image
Principles of TEM image formation Principles of TEM image

... phase variations over the plane surface. T(x,y) = A0exp[iφ(x,y)], for simplicity : A0 = 1 Assuming that the object is thin and phase shift φ is small The approximation of the emerged wave might be described as ...
Imaging the Division Process in Living Tissue Culture Cells
Imaging the Division Process in Living Tissue Culture Cells

Selective plane illumination microscopy techniques in
Selective plane illumination microscopy techniques in

Cell Mechanics
Cell Mechanics

... modulus E can be determined by recording and analyzing force-distance-curves. In order to avoid damages of living cells during the measurement and to have well-defined probe geometry for the following calculation of the moduli, we modify commercially available cantilevers by gluing a small polystere ...
Partially coherent image formation with x
Partially coherent image formation with x

... an x-ray CCD camera. Because zone plates are diffraction optics, they have a strong chromatic error, and the image quality depends on the monochromaticity of the image-forming light. To provide the required monochromaticity, a pinhole is put in close proximity to the sample plane. This pinhole toget ...
Birla Institute of Technology and Science, Pilani and Elite School of Optometry
Birla Institute of Technology and Science, Pilani and Elite School of Optometry

O 28: Plasmonics and Nanooptics IV: Light
O 28: Plasmonics and Nanooptics IV: Light

... Surface Plasmon Polaritons (SPPs) are evanescent coherent wave packets that can both confine the energy of light to metallic nanostructures and transport energy over mesoscopic distances [1]. They can then be used to generate and process information coded as optical signal to realize nanometer-scale ...
Medical Imaging Group Research Contributions/Areas
Medical Imaging Group Research Contributions/Areas

... Biomedical optical imaging enables continuous monitoring of disease (bed-side), which is highly desirable in the clinic, as optical imaging equipment are portable and non-ionizing. The challenging task here is that the quantitative accuracy provided by the reconstructed images depend on the reconstr ...
The optical microscopy with virtual image breaks
The optical microscopy with virtual image breaks

a 100-fold improvement in lithography resolution realized
a 100-fold improvement in lithography resolution realized

... negative refraction of metamaterials. He proposed that perfect imaging could be achieved with ordinary positive refraction materials. In offering his proposal, Leonhardt theoretically analyzed the MFE and demonstrated that its focus is not restricted by the diffraction limit. If Leonhardt’s theory w ...
Confocal Microscopy - Emory Physics
Confocal Microscopy - Emory Physics

Phase contrast microscopy (PCM) represents a major breakthrough
Phase contrast microscopy (PCM) represents a major breakthrough

... Figure 10b reveals the well known glowing edges associated with phase contrast, generally known as “halo” effects. This interesting phenomenon happens whenever light from such edges scatter strongly such that portions of ...
Laser beam shaping in industrial applications Wei-Yuen Tan 84717925
Laser beam shaping in industrial applications Wei-Yuen Tan 84717925

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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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