• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Lecture 5
Lecture 5

... For a source of finite size light will arrive with a different phase from different parts of the source! ...
Microscopy Tutorial - Fred Hutch Shared Resources
Microscopy Tutorial - Fred Hutch Shared Resources

... epifluorescence), and by the wavelength of light, but other optical components, as well as the imaging device, may alter the final resolution. It is important to note that resolution is not the ability to see small objects, but rather to separate very close objects. The ability to see small objects ...
Tabletop nanometer extreme ultraviolet imaging in an
Tabletop nanometer extreme ultraviolet imaging in an

... about the sample that are in excellent agreement with atomic force microscopy (AFM) and scanning electron microscopy (SEM) images, and also removes all negative effects of nonuniform illumination of the sample or imperfect knowledge of the sample position as it is scanned [17]. The result is a gener ...
Urban - TEM aberration correction review
Urban - TEM aberration correction review

... function retrieval techniques. There of this type of defect on the elecTi are a number of variants, but all have tronic properties. Such measurements in common that the microscope is are not possible in uncorrected instruoperated as an interferometer, which ments because the intensity measured explo ...
Image formation with broad bundles of rays
Image formation with broad bundles of rays

... The change in phase along different rays between points of intersection with two given wave surfaces is the same. The total change in phase between the points O and O’ is the same for the different rays. The optical path length y is the same for all these rays. ...
Fluorescence Fidelity Depends on Filters
Fluorescence Fidelity Depends on Filters

laser2-broadening
laser2-broadening

... spectroscopy. Narrow lines are highly desirable for both absorption and emission because they reduce the possibility of interference due to overlapping spectra. The line width ½ of an atomic absorption or emission line is defined as its width in wavelength units when measured at one half the ...
Optoniks
Optoniks

... We are working with: Intel and National Science Foundation (NSF) OPTONIKS has completed a 3D profilometry system prototype for Intel metrology lab which is responsible for measurement requirements and requests from other departments within Intel and also develops and assesses new metrology systems a ...
McDonald-etal-OE-2015-3D-mapping-of-intensity
McDonald-etal-OE-2015-3D-mapping-of-intensity

- Europhysics News
- Europhysics News

From near-field optics to optical antennas
From near-field optics to optical antennas

... the first single-molecule fluorescence images on a surface.9 Although spectra of single molecules had been detected years earlier, it was near-field imaging that jump-started the field of single-molecule biophysics. Ironically, shortly after Betzig’s breakthrough experiment, researchers realized tha ...
Atmospheric Monitoring for High Energy Gamma Ray Cherenkov
Atmospheric Monitoring for High Energy Gamma Ray Cherenkov

Time of flight-photoemission electron microscope for ultrahigh
Time of flight-photoemission electron microscope for ultrahigh

... 400 nm laser is a 2PPE process. This can be seen in the spectra (figure 7), which end at a final state energy of 2hν = 6.2 eV. As a consequence of the part of the spectrum closest to the work function cut-off, there are many more secondary electrons located than the spectra obtained with Hg lamp exc ...
here - TCD Maths home - Trinity College Dublin
here - TCD Maths home - Trinity College Dublin

FA15Lec16 Optical Trap
FA15Lec16 Optical Trap

... Optical Tweezers is an ultrasensitive technique ...
PPT Version - OMICS International
PPT Version - OMICS International

... three dimensional image of optical scattering from internal tissue microstructures. Michelson interferometer is used to perform low-coherence interferometry OCT measures intensity of reflected infrared light. ...
“Beam Paths” to the “Microscope”
“Beam Paths” to the “Microscope”

... Cross-section through an ∞ corrected Microscope Eyepiece ...
Provedení, principy činnosti a základy výpočtu pro výměníky tepla
Provedení, principy činnosti a základy výpočtu pro výměníky tepla

Integration of a semiconductor laser source on silicon
Integration of a semiconductor laser source on silicon

Syllabus
Syllabus

Characterisation of the Tunable Laser Source
Characterisation of the Tunable Laser Source

Optical microscope - Frank`s Hospital Workshop
Optical microscope - Frank`s Hospital Workshop

3D AND MULTISPECTRAL IMAGING FOR SUBCUTANEOUS
3D AND MULTISPECTRAL IMAGING FOR SUBCUTANEOUS

Material Chemistry
Material Chemistry

... radius, the absorption and the emission wavelengths of the nanoparticles shift to a shorter wavelength (toward UV). • The quantum dots, therefore, offer themselves as fluorophores where the emission wavelength can be tuned by selecting appropriate-size nanocrystals. • By appropriate selection of the ...
Slide 1
Slide 1

< 1 ... 47 48 49 50 51 52 53 54 55 ... 61 >

Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report