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3-D wave structuring and applications
3-D wave structuring and applications

... •The main drive for optical computing was the correlator, which exploits the high parallelism. At that time this was competitive with electronics but progress of the latter deprived the advantage of optics due to its limitations. •Apart from optical communication optical storage became probably the ...
852_1.pdf
852_1.pdf

... gel specimen, SAW velocity is 40.9 cm/s. These experiments demonstrate that our lowcoherence optical probe may be used to identify a scattering surface and detect acoustic transients excited by absorption of pulsed laser radiation. Acoustic waves generated due to photoelastic effect in subsurface ch ...
Electron
Electron

... Observations of dislocations and lattice images • 1956 independent observations of dislocations by: Hirsch, Horne and Wheland and Bollmann -Started the use of TEM in metallurgy. ...
Visible Wavelength Fiber Bragg Grating Arrays for
Visible Wavelength Fiber Bragg Grating Arrays for

... Biomedical researchers have shown that laser induced autofluorescence at 500 to 800 nm can detect cancer in unstained tissue [2]. Existing technologies have not combined sufficient spatial, spectral, and temporal resolution in one instrument. Spectrometer acquisition speeds are not fast enough to ge ...
Two-photon ablation with 1278nm laser radiation
Two-photon ablation with 1278nm laser radiation

Frequency-Domain Spectroscopy Using 280 nm and 300 nm
Frequency-Domain Spectroscopy Using 280 nm and 300 nm

... In the last few decades, fluorescence methods have become extremely important in many areas of science and technology including analytical chemistry, biophysics, cell biology, clinical chemistry and biotechnology, to name but a few. Determinations of the excited state lifetimes of fluorescent molecu ...
Near-field amplitude and phase recovery using phase
Near-field amplitude and phase recovery using phase

Total 3D imaging of phase objects using defocusing microscopy
Total 3D imaging of phase objects using defocusing microscopy

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PDF

STED Microscopy
STED Microscopy

Precision High Numerical Aperture Scanning System for
Precision High Numerical Aperture Scanning System for

... occurs in the focal region of the laser and is dependent on the speed the focal region is travelling through the material and power in the laser. In order to write efficiently, it is crucial to be able to reach scan speeds of up to 200 mm/s with reliable and consistent knowledge of the speed and pos ...
X-ray phase contrast microscopy at 300 nm
X-ray phase contrast microscopy at 300 nm

The Electron Microscope as an Illustration of the Wave Nature of the
The Electron Microscope as an Illustration of the Wave Nature of the

... especially the confocal optical microscope, have enabled the optical microscope to achieve a resolution close to the theoretical limit. Contrast formation The most important difference is that contrast is formed in different ways. In the light microscope, we usually view differences in the absorptio ...
Deriving Abbe`s Certainty from Heisenberg`s Uncertainties Ernst HK
Deriving Abbe`s Certainty from Heisenberg`s Uncertainties Ernst HK

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Noninterferometric single-shot quantitative phase
Noninterferometric single-shot quantitative phase

... (S  0.7 ∼ 0.8). For TIE phase measurements, the results are largely independent of the condenser setting (especially for the low spatial frequency components) [15]. However, in the SQPM, we prefer to narrow down the condenser aperture a bit (S  0.3 ∼ 0.4) to ensure a certain level of spatial coher ...
Temporal Integrated Detection and Applications of FS-Pulse Scattering S. Bakic
Temporal Integrated Detection and Applications of FS-Pulse Scattering S. Bakic

... the theoretical and numerical description of the scattering process [1], whereas the applications mainly focus on the inelastic scattering after stimulation with laser light. Up to now, the special characteristics of short pulse elastic scattering, with pulse lengths shorter than the object’s extent ...
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13 Int. Symp on Appl. Laser ...

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File - ce

Optimizing Fluorescence Signal Quality
Optimizing Fluorescence Signal Quality

ps fiber laser for CARS-authorproof-v2
ps fiber laser for CARS-authorproof-v2

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Lect03_Bi177_MicroscopeOptics

... • Velocity (or speed) at which a wave travels can be calculated from the wavelength and frequency. ...
Chester F - RIT Center for Imaging Science
Chester F - RIT Center for Imaging Science

Using Transmission Electron Microscopy (TEM) for Chemical
Using Transmission Electron Microscopy (TEM) for Chemical

... requires one to form images with electrons diffracted into an annular aperture centered on, but not including, the unscattered beam. As a result, the field around the specimen (i.e. where there is no specimen to scatter the beam) is generally dark. Dark field studies in transmission electron microsc ...
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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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