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Adaptive Optics and the cone mosaic
Adaptive Optics and the cone mosaic

... cones participate in detection. Shown are the percentages of white and colored responses that were placed in each response category, interpolated at 50% frequency of seeing. Percentages for BS are white, 56%; red, 42%; yellow-green, 0.7%; green, 0.7%; blue-green, 0.3%; and blue, 0.8%. In addition to ...
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Single-pulse coherent anti-Stokes Raman scattering microscopy
Single-pulse coherent anti-Stokes Raman scattering microscopy

... been demonstrated [16], the practical applicability to biological samples has not yet been achieved. We apply FT-CARS microspectroscopy to imaging an unstained HeLa cell and obtain multi-spectral images from the CARS spectra. We also show that the difference between CARS spectra can be quantified by ...
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... ferromagnetic materials and provide a spatially resolved non-invasive approach that allows for higher temporal resolution than other commonly used methods1. There has been great interest recently in the magnetization reversal dynamics of single domain nanomagnets from both a scientific perspective a ...
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... of free electrons at metal/dielectric interface. As a wave phenomenon, surface plasmons can be focused by using appropriate excitation geometry and metallic/dielectric structure. The strong spatial confinement and high field enhancement make surface plasmon very attractive for near-field optical ima ...
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Optical diffraction tomography for high resolution live cell imaging

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10.3.2.1.1 Spectral apparatus An optical arrangement or an

... 10.3.2.1.1 Spectral apparatus An optical arrangement or an instrument which disperses optical radiation into a spectrum and/or isolates a specific spectral band is termed spectral apparatus or a spectral instrument. If the entrance aperture, which may be a slit, is sharply imaged in both dimensions, ...
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Coherent Optical Information Systems
Coherent Optical Information Systems

... bate at ␭ ⫽ 514 nm (19). The maximum reflecoccurs as different portions of this broad specavailability of coherent light sources has been tivity is at the Bragg wavelength ␭ ⫽ 1550 nm trum reach the destination with different delays. the key innovation enabling the rapid rise of (Fig. 4). Notice tha ...
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How to turn your microscope into a phase contrast microscope
How to turn your microscope into a phase contrast microscope

... through the entire objective. The interfering effect from the disk is passing through a ‘lens’ that replaces the disk, so that the diameter of this beam is much smaller. Now, consider an object in the proper focus point of the microscope. The light that passes through the entire objective is focusse ...
Ultrahigh-resolution optical coherence tomography
Ultrahigh-resolution optical coherence tomography

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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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