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Optical pulse generation using a low-voltage electro-optic
Optical pulse generation using a low-voltage electro-optic

Diffraction limited resolution - X-ray and Observational Astronomy
Diffraction limited resolution - X-ray and Observational Astronomy

lecture 3 Introduction to Laser
lecture 3 Introduction to Laser

... Mode-locking is a technique in by which a laser can be made to produce pulses of light of extremely short duration, on the order of picoseconds (10−12s) or femtoseconds (10−15s). Need for Mode Locking When laser is oscillating with various modes and if modes are uncorrelated The output intensity i.e ...
Imaging properties of supercritical angle
Imaging properties of supercritical angle

... of the optics (paraboloid glass segment, solid immersion lens). For this situation, there is no straightforward method available for computing the point spread function. As far as we know, all previously described methods for calculating optical systems with high numerical aperture use the property ...
Approximate Theory of Rectangular Optical Waveguides
Approximate Theory of Rectangular Optical Waveguides

Absorption Measurements on PC1
Absorption Measurements on PC1

Modulation Transfer Function
Modulation Transfer Function

... where C(s) is the object contrast and C 0 (s0 ) the image contrast. A value of 1 means that frequency is perfectly transferred to the image plane, which is the best possible outcome in a passive system. A value of 0 means that frequency will not appear at all in the image. The object- and image-spac ...
Laser Distance and Speed Detection
Laser Distance and Speed Detection

Optical Sources
Optical Sources

... This laser is called a Fabry-Perot laser diode (FP-LD) Semiconductor-air interface produces a reflection coefficient at normal incidence of ...
Imaging Laboratory Exercise Scanning Electron Microscope
Imaging Laboratory Exercise Scanning Electron Microscope

... metal, such as gold, before it is imaged in the microscope. Because of this, these devices have a limited application, especially for examining dynamic samples, such as in life forms. In both SEM and TEM electrons are generated in the portion of the microscope that is referred to as the electron gun ...
Microscopes - Biozentrum
Microscopes - Biozentrum

... properties of the medium. Changes in amplitude give rise to familiar absorption of light which gives rise to colours when it is wavelength dependent. The human eye measures only the energy of light arriving on the retina, so changes in phase are not easily observed, yet often these changes in phase ...
Microscopes - Photonics Research Group
Microscopes - Photonics Research Group

... in the image plane. The sum of the two diffraction images, assuming the two points of light were mutually incoherent, appears as in Fig. 7a . As d becomes smaller so that the first minimum of one diffraction image overlaps with the central maximum of the neighboring diffraction image (d 5 rAiry, Fig ...
Technology for a better society
Technology for a better society

... Has the same effect but is a property of the lens itself, and so may be either more or less restricting than A(u). The aberration function B(u): Is usually expressed as ...
Orbital Dynamics of the Laser Interferometer Space Antenna
Orbital Dynamics of the Laser Interferometer Space Antenna

Introduction to Optical Engineering and Design ENSC 376
Introduction to Optical Engineering and Design ENSC 376

acknowledgements
acknowledgements

... Fig. 4 a) Part of a IR beam written DOE. b) IR optical reconstruction of DOE in a). Thermal image was detected by liquid crystals.Central part of the image was blocked by a mirror. Setup to make the reconstruction is shown in Fig. 5. The second example that is of interest used the ellipse of Figure ...
laser - SlideBoom
laser - SlideBoom

Transmission Electron Microscopy
Transmission Electron Microscopy

... 1951 – R. Castaing develops the X-ray Energy Dispersive Spectroscopy (XEDS). 1970 – The first High Resolution Transmission Electron Microscopy (HRTEM) images are obtained. The spatial resolution, about 0.4 nm, reveals details from the sample’s atomic structure. 1998 – Aberration corrected HRTEM achi ...
File - GENERAL DEPARTMENT
File - GENERAL DEPARTMENT

Total Internal Reflection Microscopy
Total Internal Reflection Microscopy

... experiments with glass microscope slides in aqueous solutions. Because of this exponential sensitivity, a very small change in h produces a measurable change in intensity. For example, we estimate that the photomultiplier tube we use to quantify the light intensity can realistically detect a 1% chan ...
Coherent light sources and optical techniques for Thomson
Coherent light sources and optical techniques for Thomson

... called Pockels effect), inducing some birefringence in a non-linear crystal. This effect can be also inducted by the transverse electric field of a ultra relativistic electron beam. If a polarized probe laser is used, by measuring its polarization variation, It’s possible to derive the electron beam ...
Photon mapping of individual Ag particles on MgO/Mo(001)
Photon mapping of individual Ag particles on MgO/Mo(001)

... Abbes diffraction limit,1 two pointlike photon sources can only be distinguished if their mutual distance is larger than half the wavelength of the emitted radiation divided by the refractive index of the environment. The achievable resolution is therefore of the order of 250 nm (λrad ∼ 500 nm), hen ...
Digital Fourier Microscopy for Soft Matter Dynamics
Digital Fourier Microscopy for Soft Matter Dynamics

... should be aware that the term microscopy is used here in a very broad sense because it includes many situations where, due to defocusing [35], speckles [17] or other effects the image of the sample does not seem to bear any resemblance with the sample itself. It is worth concluding this Introduction ...
RESOLUTION OF ICCD CAMERAS
RESOLUTION OF ICCD CAMERAS

... obtainable from the point spread function (PSF) or line spread function (LSF), which are discussed in a later section, by a Fourier transform. The MTF is usually presented as a graph showing the modulation transfer function versus spatial frequency which is customarily specified in line pairs per mi ...
Adiabatic far-field sub-diffraction imaging ARTICLE Hu Cang *, Alessandro Salandrino
Adiabatic far-field sub-diffraction imaging ARTICLE Hu Cang *, Alessandro Salandrino

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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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