• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Photoelectron imaging of modal interference in
Photoelectron imaging of modal interference in

Quantitative evaluation of light microscopes based on image
Quantitative evaluation of light microscopes based on image

Optimal wavelength for ultrahigh-resolution optical
Optimal wavelength for ultrahigh-resolution optical

... of use. The central and limbal regions of the eye were scanned. Figure 5(a) shows the image of the corneal epithelium at the center of the eye. The image size is 0.3 x 0.2 mm. The measured epithelium thickness is approximately 45 µm which correlates with reported results [13]. Figure 5(b) shows an i ...
Ultrafast Optics: Tools and Techniques
Ultrafast Optics: Tools and Techniques

In Situ Imaging of Cold Atomic Gases
In Situ Imaging of Cold Atomic Gases

Digital holography of total internal reflection
Digital holography of total internal reflection

Quasi-3D plasmonic coupling scheme for near-field optical lithography and imaging Y W
Quasi-3D plasmonic coupling scheme for near-field optical lithography and imaging Y W

What is total internal reflection?
What is total internal reflection?

... of the classroom project: Day 1: A brief introduction of Lasers, definitions and terms. An introduction to laser safety will be presented (possibly a pre-test). The students must first pass the laser safety test (will allow two days for preparation for this test). Day 2: Engineering Design lesson – ...
Improving fluorescence detection in lab on chip devices
Improving fluorescence detection in lab on chip devices

Soft X-ray tomography and cryogenic light microscopy - X
Soft X-ray tomography and cryogenic light microscopy - X

Lecture 04
Lecture 04

... diffraction and interference • In Wikipedia, the diffraction phenomenon is defined as the interference of waves according to the HuygensFresnel principle ...
Microsoft Office PowerPoint 2003 Beta - poster#2
Microsoft Office PowerPoint 2003 Beta - poster#2

4.6 Optical Fibres
4.6 Optical Fibres

The pinhole camera
The pinhole camera

Enhanced visualization of choroidal vessels using ultrahigh
Enhanced visualization of choroidal vessels using ultrahigh

... compared to 10% at ~800 nm for a double pass of the optical beam through a human eye. This will result in relative sensitivity loss of 2.4 dB when imaging at ~1060 nm and 21 dB at ~1300 nm. However, according to the ANSI standard10, the maximum permissible light exposure in the eye increases with wa ...
PhotoAcoustic Schlieren Elastography II
PhotoAcoustic Schlieren Elastography II

Microscope Project for Undergraduate Laboratories Abstract
Microscope Project for Undergraduate Laboratories Abstract

Wave optics theory and 3-D deconvolution
Wave optics theory and 3-D deconvolution

The principles of statistical optics and image formation A Statistical
The principles of statistical optics and image formation A Statistical

... A statistical model is required for the description of the discrete nature of the interaction between light and matter and the presence of internal electronic detector noise. In summary, statistical processes are observed in (i) illumination (emittance), (ii) transmission, (iii) image formation, (iv ...
[pdf]
[pdf]

Nanofabrication with Holographic Optical Tweezers
Nanofabrication with Holographic Optical Tweezers

... methods for creating2–4 large arrays of optical tweezers in arbitrary arrangements by using computer-designed diffractive optical elements to configure the necessary pattern of laser beams. Such holographic optical tweezer (HOT) arrays can be used to assemble large numbers of colloidal particles int ...
Direct measurement of standing evanescent waves with a photon
Direct measurement of standing evanescent waves with a photon

Fundamental Limits in Confocal Microscopy
Fundamental Limits in Confocal Microscopy

Light Sources * II The Laser
Light Sources * II The Laser

Wave Optics Theory and 3-D Deconvolution for the Light Field
Wave Optics Theory and 3-D Deconvolution for the Light Field

< 1 ... 41 42 43 44 45 46 47 48 49 ... 61 >

Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report