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BMS 631 - Lecture 4
BMS 631 - Lecture 4

Microsoft Word Format - University of Toronto Physics
Microsoft Word Format - University of Toronto Physics

... mode at low power, reflect some of the laser beam into the spectrometer so as to measure the central wavelength of the light. Use the spectrometer with the entrance slit narrowed and without the focusing lens, in order to avoid overloading the detector. Make a search at higher sensitivity for other ...
PDF
PDF

... laser beam to be split into many beams, each forming an individual optical trap. By updating the SLM with a sequence of holograms, the individual optical traps can be moved in all three dimensions (3D) [10]. Considerable work has been reported on forming 3D arrays of particles and also modifying the ...
Ingen lysbildetittel - Department of Telematics
Ingen lysbildetittel - Department of Telematics

... State changed though phase shifts or by switching in space. Linear optical elements can be used without major problems (determinsitic), but loss and noise will destroy the system ...
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The `IEC` LASERVIEW – kit of shapes

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Barium Ions for Quantum Computation

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Criteria for Optical Systems: Optical Path Difference • Optical Path

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Prepared By - ichapps.com

Image formation and optical transfer function in a course of
Image formation and optical transfer function in a course of

... We have also computed the image of a five-bar object. The dimensions of the bars are 0.52 nn and the period is 1.04 .tm. The frequency of this five-bar object in the units used in Eq.(2) is 0.702 for NAO.5 and &=O.365tm. Fig. 4 shows the images of the five-bar test given by a system without aberrat ...
RECOMMENDATION ITU-R BT.1201- Extremely high resolution
RECOMMENDATION ITU-R BT.1201- Extremely high resolution

... temporal resolution and others, can be set, in principle according to the demands. However, creation of moving images on a real-time basis is difficult to realize with current technology. It depends on the complexity of the image to be produced and the CG technology used. Image generation by simple ...
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PDF - Grueber Lab

... Conventional light-sheet imaging techniques illuminate the sample from the side using a thin sheet of light, and acquire images of the illuminated plane using an orthogonally aligned second objective3-5. The sample is then translated relative to this co-aligned plane to form a 3D volumetric image. T ...
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Calculations Table 1: Single Slit

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Week 9 Wed. (Lesson 15) Coherence and Optical Tomography

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Design for a Compact Tunable Ti:Sapphire Laser.

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Enhancement of image quality and imaging depth with Airy light

... programmed to display the appropriate phase mask for either Gaussian or Airy illumination. For a Gaussian light-sheet, the phase profile across the pupil is uniform, for an Airy light-sheet the phase profile is described by P(u, v) = exp (2πiα[u 3 + v 3 ]), where u and v are normalised pupil coordin ...
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Optical Coherence Tomography as a diagnostic tool

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Real-time digital holographic microscopy Ventseslav Sainov and Elena Stoykova

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Basic Laboratory Materials Science and Engineering Scanning Electron Microscopy

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Tunable External-Cavity High

Experimental method for reliably establishing the refractive index of
Experimental method for reliably establishing the refractive index of

... iridescent beetle C. vittata, he obtained layer thicknesses from TEM images and optical data from a Zeiss microspectrophotometer. He then derived values for the refractive indices of the layers by comparing the wavelength of maximum reflectance in his experimental data to that obtained using the opt ...
MINIATURIZED FLUORESCENCE EXCITATION PLATFORM WITH OPTICAL FIBER FOR BIO-DETECTION CHIPS
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Imaging scanning tunneling microscope
Imaging scanning tunneling microscope

... The STM electroluminescence–Fourier space imaging approach allows excitation of localized and propagating SP modes in nano-structured metal films using a nano-scale source, and also allows separate mapping of different SP modes. Fourier space imaging avoids some complications of considering optical ...
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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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