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Midterm2
Midterm2

Motion and Optical Flow
Motion and Optical Flow

LAB 1 - SIMPLE DIFFRACTION, FOURIER OPTICS AND ACOUSTO
LAB 1 - SIMPLE DIFFRACTION, FOURIER OPTICS AND ACOUSTO

... This will badly blur the image of the laser beam on the pinhole and allow some laser light to get through the pinhole when the spatial filter is placed in the optical setup. Place the spatial filter on a base, and put it in the path of the laser beam with the back (iris side) facing the laser. Adju ...
chapter 7
chapter 7

... nm, which is considerably less than that of visible light (380-760 nm). Consequently, two neighbor electron beams of such energy interfere when they are at a much shorter distance from each other. Therefore, the electron microscope can reach a resolution of about 0.2 nm, which is about 1000 times gr ...
LATTICE IMAGING IN TRANSMISSION ELECTRON MICROSCOPY
LATTICE IMAGING IN TRANSMISSION ELECTRON MICROSCOPY

... The last decades are characterized by an evolution from macro- to micro- and more recently to nanotechnology. Examples are numerous, such as nanoparticles, nanotubes, quantum transistors, layered superconducting and magnetic materials, etc. Since many material properties are strongly connected to th ...
Large-area picosecond laser-induced periodic surface
Large-area picosecond laser-induced periodic surface

Enhanced transmission in near-field imaging of layered plasmonic
Enhanced transmission in near-field imaging of layered plasmonic

Conductive atomic force microscope for investigation of thin-film gate insulators G. WIELGOSZEWSKI
Conductive atomic force microscope for investigation of thin-film gate insulators G. WIELGOSZEWSKI

Strategies for the compensation of specimen
Strategies for the compensation of specimen

... The main advantage of the confocal microscope lies in its ability to image efficiently only those regions of an object which lie close to the focal plane. This optical sectioning ability permits through-focus series of optical slices to be recorded, which can then be processed to render the volume o ...
Leica TCS SP8 STED 3X-Brochure EN
Leica TCS SP8 STED 3X-Brochure EN

1 Introduction 2 Theory of Optical Trapping
1 Introduction 2 Theory of Optical Trapping

Nanofabrication via Atom Optics with Chromium
Nanofabrication via Atom Optics with Chromium

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PDF

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Opto-Mechanical Image Quality Degradation of Single Point
Opto-Mechanical Image Quality Degradation of Single Point

... where  is laser source wavelength in microns n is the refractive index of the transparent or reflective material under test. i is the angle of incidence on sample is the total effective rms surface roughness, in microns, over frequencies from 0 to 1/. s, r is the scattered and reflectance ...
Microscopy corrected
Microscopy corrected

Sluggish light for radio-frequency true-time-delay
Sluggish light for radio-frequency true-time-delay

... prisms. These schemes require an additional spatial dimension for manipulating different optical frequencies by applying appropriate linear phase factors with a prism or tilted mirror, and these are not suitable for beam forming of two-dimensional (2D) RF phased-array antennas that have already used ...
10.2 Fourier Transform Infrared Spectroscopy
10.2 Fourier Transform Infrared Spectroscopy

... To obtain an infrared spectrum via a Fourier transform, the signal would be recorded (digitized) as a function of time to obtain F(t). Then the spectrum would be computed using the following expression, ...
Optical frequency standard
Optical frequency standard

... than those with lower oscillation frequencies. Although optical sources are used for some functionalities (optical pumping, laser cooling of atoms), all present primary and secondary frequency standards are "microwave-based” as they probe an atomic transition whose frequency belongs to the microwave ...
Optical Data Storage.pdf
Optical Data Storage.pdf

... has recently been proposed by the Zyss group which encodes information by an all-optical poling technique in which the angles of polarization of the two irradiating fields are varied. The resulting spatial changes in the symmetry of the quadratic susceptibility tensor lead to a modulation of the det ...
Generation of a dark hollow beam by a four
Generation of a dark hollow beam by a four

Seminar2
Seminar2

... • After many simulations we chose a cavity and a mirror, for which the simulation gave the present NF intensity profile ...
Diode-Array UV Solar Spectroradiometer Implementing
Diode-Array UV Solar Spectroradiometer Implementing

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Direct Laser Writing: Versatile Tool for Microfabrication of Lithium
Direct Laser Writing: Versatile Tool for Microfabrication of Lithium

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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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