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Measuring Human Chromosomes using Confocal microscopy
Measuring Human Chromosomes using Confocal microscopy

Saturated 13.2 nm high-repetition-rate laser in nickellike cadmium
Saturated 13.2 nm high-repetition-rate laser in nickellike cadmium

High-precision, high-accuracy ultralong-range swept
High-precision, high-accuracy ultralong-range swept

Brightfield contrast methods
Brightfield contrast methods

Optical Activity
Optical Activity

... were produced. Laboratory synthesis always produces both types. However, in the first living systems on earth, left or right-handed activity could have been adopted purely as a matter of chance and, once a particular asymmetry was established, it maintained itself. This conjecture implies that optic ...
Pulsed-source and swept-source spectral-domain optical coherence tomography with reduced motion artifacts
Pulsed-source and swept-source spectral-domain optical coherence tomography with reduced motion artifacts

Physical Optics - Haverford College
Physical Optics - Haverford College

... measurement of the diameter D of different size pinholes using the observed Airy disk pattern is part of this lab. One good approximation of a point source is a bright star. A pair of stars close to one another can give a measure of the diffraction limits of a system which is called the Rayleigh res ...
Controllable Optical Phase Shift Over One Radian from a Single
Controllable Optical Phase Shift Over One Radian from a Single

Low threshold edge emitting polymer distributed feedback laser based on
Low threshold edge emitting polymer distributed feedback laser based on

... two characteristics that make them ideal candidates for solidstate lasers in the visible spectral range.1,2 To this end, multiple resonator configurations have been suggested, such as microcavities,3 microdisk lasers,4 and distributed feedback 共DFB兲 structures.5–10 The latter are particularly attrac ...
Quantitative force measurements with optical tweezers: The JPK
Quantitative force measurements with optical tweezers: The JPK

... light with the scattered light from the trapped particle, collimated by a detection lens, can be used to get a sensitive measure for the displacements of the trapped particle from the focus. If the trap steering scheme is wellchosen, the intensity distribution in the back focal plane (BFP) of the co ...
Ultra–large field-of-view two-photon microscopy
Ultra–large field-of-view two-photon microscopy

... transcranial window [12], to resolve individual vessels and cells [13]. The design of a scanning microscope entails trade-offs between mechanical and optical characteristics to achieve the desired speed, resolution, field-of-view, and experimental flexibility. Galvanometric mirrors that are capable ...
optical computer - 123SeminarsOnly.com
optical computer - 123SeminarsOnly.com

Loss measurements on semiconductor lasers by
Loss measurements on semiconductor lasers by



Paper Report on “Optical Computing: Need and Challenge”
Paper Report on “Optical Computing: Need and Challenge”

Optical Gain Experiment Manual
Optical Gain Experiment Manual

Special Optical Elements
Special Optical Elements

A Laser Coupler for DVD/CD Playback
A Laser Coupler for DVD/CD Playback

Ultrafast and High Resolution Crack Detection Using Fully
Ultrafast and High Resolution Crack Detection Using Fully

... 1. Introduction Photonic time-stretch (PTS) technique, also known as dispersive Fourier transform [1], has enabled ultrafast interrogation of fiber Bragg grating (FBG) sensors with unprecedented measurement speed of tens of MHz [2]. This is made possible by using chromatic dispersion to largely stre ...
Image Quality Criteria - University of Arizona
Image Quality Criteria - University of Arizona

... quality metric or metrics used to establish the image quality criteria of an imaging system. Figure 7 shows the MTF for several aberration free optical systems. However, each optical system has a varying size of central obscuration. It is noticed in the plot that the MTF drastically decreases as the ...
Laser Molecular Spectroscopy CHE466 Fall 2007
Laser Molecular Spectroscopy CHE466 Fall 2007

... The efficiency of a laser is related to its ability to sustain a population inversion and regenerate a population in the low energy state to initiate a new pumping cycle. As you may see a 2 level laser would be very inefficient, because a population inversion may not be acquired as a result of popul ...
Optically induced forces in scanning probe microscopy
Optically induced forces in scanning probe microscopy

Enkeltmolekylstudier av proteiner som bidrar til - Innsida
Enkeltmolekylstudier av proteiner som bidrar til - Innsida

... (Peak Force Quantitative Nanomechanical Mapping) as mode for using atomic force microscopy. This mode, different from more conventional known ones such as contact, non-contact or tapping mode, potentially allows correlations between the morphological features (height variations) and the resulting me ...
Noise-equivalent sensitivity of photoacoustics
Noise-equivalent sensitivity of photoacoustics

Attosecond angular streaking - the Max Planck Institute for the
Attosecond angular streaking - the Max Planck Institute for the

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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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