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... Pupil diameter intensity of a diffraction – limited – spot PSF have multiple peaks  2 or more point images for single point ...
A History of Imaging
A History of Imaging

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Hyperfine Structure of Rubidium

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A high resolution ion microscope for cold atoms

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Imaging and focusing of an atomic beam with a large period

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Dynamic measurements using a Fizeau

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CH332 Lab 4: FLUORESCENCE LIFETIME OF QUININE SULFATE

... 2. Compare and contrast the two fluorescence methods used in this lab (scans vs lifetime). Be specific regarding the information obtained in each experiment and differences in instrument operation. 3. How could this instrument be used to analyze two different compounds with similar emission and exci ...
The Wave-Front Aberration Polynomial
The Wave-Front Aberration Polynomial

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Get PDF - OSA Publishing

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... development was the application of a flow cuvette in which the cells were moving through the detection volume3. These early microscope based flow cytometers used microscope objectives with low numerical aperture in combination with a cylinder lens to obtain an asymmetrical, slit-like shaped focus. I ...
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to - UCL Medical Physics and Biomedical Engineering

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Excited State Processes and Application to Lasers The technology

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The present work gives recommendations for rational - Dimka

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A high numerical aperture (NA = 0.92)

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WHAT IS THE OPTICAL COMPUTING?

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Demonstration of Optical Rotatory Dispersion of Sucrose

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Inverse scattering for frequency-scanned full-field

... around the focal plane is obtained, and the out-of-focus contributions are removed by coherence gating. The usual technique is then to translate the sample through the focal plane so that the scatterers at many different depths may be imaged and a 3-D structure obtained. While this method can be use ...
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BMS 631 - Lecture 4

Microsoft Word Format - University of Toronto Physics
Microsoft Word Format - University of Toronto Physics

... mode at low power, reflect some of the laser beam into the spectrometer so as to measure the central wavelength of the light. Use the spectrometer with the entrance slit narrowed and without the focusing lens, in order to avoid overloading the detector. Make a search at higher sensitivity for other ...
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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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