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Lecture 1
Lecture 1

Initial demonstration of a local, evanescent, array coupled biosensor concept
Initial demonstration of a local, evanescent, array coupled biosensor concept

Microwave frequency generation using a dual
Microwave frequency generation using a dual

... such as soliton pulse train generation [1], heterodyne interferometry for distance measurement [2], and optical sensing [3]. Dual-mode lasers are also of interest for their potential in generating radio-frequency signals for microwave applications. Radio-frequency (RF) beat signal has been demonstra ...
Close-range  videometry  -
Close-range videometry -

... The two halves of the sensor form the two images of the imaginary cameras. The interior orientation denotes the estimation of the parameters in the functional camera model. The functional model of the system describes the irnaging of an object point past the mirrors, through the lens with its distor ...
Simulating and optimizing compound refractive lens-based X
Simulating and optimizing compound refractive lens-based X

... with respect to N’. While attenuation by the lens means that CRLs practically operate within the first half-period (i.e. 0  N’  ), optical behavior differs markedly between the thin-lens limit (i.e. NT  fN and correspondingly N’  1) and the general thick lens case (i.e. all values of N’). This ...
Principles of Electron Structure Research at Atomic Resolution
Principles of Electron Structure Research at Atomic Resolution

Analysis of the Spectral Resolution of a TeO2 based Noncollinear
Analysis of the Spectral Resolution of a TeO2 based Noncollinear

Chapter 7 Optical lattices and experiment design
Chapter 7 Optical lattices and experiment design

... It is imperative to take the light’s polarisation into account when calculating the dipole force introduced in Chapter 2. The first reason is that the individual laser beam’s electric fields must be added as vectors. The resulting total electric field vector can then be used to calculate the intensi ...
Chapter 11 Laser
Chapter 11 Laser

... The system then acts as a source of radiation with photon energy E. Furthermore, since the photons are the result of stimulated emission, they all have the same frequency, phase, polarization, and direction. The resulting radiation is therefore very much more coherent than light from ordinary source ...
Laser beam profiling
Laser beam profiling

Spherical Aberration. q ℓ
Spherical Aberration. q ℓ

NewPresentationii22
NewPresentationii22

Advantages of Holographic Optical Tweezers
Advantages of Holographic Optical Tweezers

Nonlinear optical properties of aromatic amino acids in the
Nonlinear optical properties of aromatic amino acids in the

... To measure the solutions’ nonlinear absorption coefficients, an intensity of one order of magnitude higher than previously, Io = 180 GW/ cm2, was used. The experimental results of the open aperture Z-scan setup are presented in Fig. 2. The real and the imaginary part of the effective third-order opt ...
Quantum Interference 1 Claude Cohen-Tannoudji Scott Lectures Cambridge, March 7
Quantum Interference 1 Claude Cohen-Tannoudji Scott Lectures Cambridge, March 7

Single Photon Sources - University of Rochester
Single Photon Sources - University of Rochester

Presentation - University of Arizona
Presentation - University of Arizona

... Cardinal points and planes-continue Nodal planes have the characteristic of identity angular magnification. When the optical system is in air, nodal points/planes coincide with the principal points/planes. Principal points/planes can be described using Newtonian equations or Gaussian equations w ...
Full text - Ward Ober Lab
Full text - Ward Ober Lab

... plane of focus (plane 1) of the objective lens. Previously, we had shown that the 3D fundamental limit of x0 in a conventional microscope setup deteriorates with increasing defocus distances and is symmetric about z0 = 0.2 In contrast, we see that the 3D multifocal plane fundamental limit exhibits a ...
Modeling, Simulation and Application of the Probe Beam Deflection
Modeling, Simulation and Application of the Probe Beam Deflection

[pdf]
[pdf]

... workstation. Finite difference calculations, on the other hand, take ,20 min of CPU time for each source position.18 Least-squared fitting procedures for determination of fluorophore concentration and lifetime based on these analytic solutions are thus possible within reasonable CPU times. ...
Optical gratings: Nano-engineered lenses - MiNa
Optical gratings: Nano-engineered lenses - MiNa

... but in a much smaller size and based on a physically different mechanism. This concept is already used to direct radiofrequency waves in phased-array antennas; in fact, the approach of using resonators to alter the phase of a reflected radiofrequency electromagnetic wave has ...
Green's function formulation for third-harmonic generation microscopy
Green's function formulation for third-harmonic generation microscopy

... With the Green’s function model we are able to investigate THG from interfaces of different orientations. Figure 4 shows that THG signals can arise from an interface perpendicular or parallel to the optical axis. Interestingly, the THG signal is dependent on the polarization direction of the excitat ...
PPT
PPT

... If the distance between adjacent pixels is less than the minimum resolvable separation due to diffraction, then diffraction limits the image quality. ...
1 - www2
1 - www2

LxxA, Overview of Microscopy methods, part a
LxxA, Overview of Microscopy methods, part a

... • Almost all modern electron microscopes use pole pieces for high resolving power and high magnification. • The function of such an electron lens is more or less the same as that of horse-shoe magnets symmetrically arranged about an axis. • Accordingly, all the parallel electron beams incident to t ...
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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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