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EXPERIMENTAL TECHNIQUES
EXPERIMENTAL TECHNIQUES

Laser Chemistry Emeritus Group - Max Planck Institut für Quantenoptik
Laser Chemistry Emeritus Group - Max Planck Institut für Quantenoptik

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... Compound light microscopes employ a series of lenses to enlarge (i.e., magnify) the image of the object being studied. Total magnification is the product of the magnifying power of the objective lens times the magnifying power of the ocular lens. Typical microscopes have four objective lenses (4X, 1 ...
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... today. You’ll learn about the clever things that can be done with light to deliver higher data rates over longer distances at lower cost than ever before. You’ll also appreciate the supporting role played by recent advances in electronic communications technology in overcoming some of the impairment ...
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... In contact AFM electrostatic and/or surface tension forces from the adsorbed gas layer pull the scanning tip toward the surface. It can damage samples and distort image data. Therefore, contact mode imaging is heavily influenced by frictional and adhesive forces compared to non-contact or tapping mo ...
Introduction to Nonlinear Optics
Introduction to Nonlinear Optics

... Second Harmonic Generation and Nonlinear Microscopy Nonlinear Optical Microscopy An important application of harmonic generation is nonlinear microscopy. . . Microscopy based on second-harmonic generation in the configuration of a confocal microscope and excited by femtosecond laser pulses was intr ...
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full article pdf

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Advances in diamond-turned surfaces enable unique cost

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Selective photodissociation of tailored molecular - Beilstein

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Nanophotonics: Shrinking light-based technology

... resolution. Yet, advances in nanophotonics research require new techniques to excite materials and probe them at the nanoscale. Although super-resolution microscopy tools such as photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), and stimulated emiss ...
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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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