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Plasmonic Nanopore for Electrical Profiling of
Plasmonic Nanopore for Electrical Profiling of

Laser Systems for Inverse Compton Scattering Gamma
Laser Systems for Inverse Compton Scattering Gamma

Unwrapping Hartman-Shack Images from Highly Aberrated Eyes
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Edge-enhanced imaging with polyvinyl alcohol/acrylamide photopolymer gratings 1510

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FTIR Instrumentation
FTIR Instrumentation

... are completely in phase with each other and, therefore, interfere constructively. The two component beams that make up the output beam passed to the source are completely out of phase with each other and, therefore, interfere destructively. In this case, all of the original light energy goes to the ...
Total Internal Reflection Fluorescence
Total Internal Reflection Fluorescence

... beam diameters, it may be useful to first expand or contract one or more of the individual beams with a telescope lens system. In addition, beam shutters can be added to individual laser lines for selection of specific excitation wavelength(s) during experiments. Depending on space constraints it ma ...
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Development of a67kHz Internal OPO Laser for Hellborne LIdar

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intropig

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Counting Airborne Bacteria in Real Time

... now being given precisely this addition with the availability of new laser light sources. The preliminary stage - flow cytometry Numerous biological systems can be easily stimulated energetically with short-wave light and react to lower frequency light with re-emission. This fluorescence effect was ...
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Palladium Ultra Thin Layer Profiles Evaluation by Evanescent Light

... DELI is an optical evanescent waves microscopy technique based on capturing the light extracted by nano layers deposited on a substrate which also serves as a waveguide [2,3]. The DELI technique used in this investigation is a far field optical microscopy measurement technique developed to achieve a ...
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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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