• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Convolution in Imaging and the Optical Transfer Function Process
Convolution in Imaging and the Optical Transfer Function Process

... So for a perfect system, there would be little to no diffraction in the transfer. Of course, it’s very difficult and expensive to emulate such a system. So, the PSF is mainly determined by diffraction (which is always limited). Assuming the optical system is linear, the image for an object consistin ...
Microscope
Microscope

Demonstrating the style for the Journal of Physics
Demonstrating the style for the Journal of Physics

... by independent slices, the intensity distribution I(k) along the k-axis of the spectrometer is modulated with spatial frequencies fkj=zj/that depend on the optical path difference between the j-th slice within the object and the reference surface. The phase difference i(k)−j(k) between light com ...
Tao Yuan, Jingzhou Xu, and Xicheng Zhang Rensselaer
Tao Yuan, Jingzhou Xu, and Xicheng Zhang Rensselaer

FA15Lec17 Optical Traps.Two
FA15Lec17 Optical Traps.Two

... light is brightest. (Slight deviation in z-direction due to reflection.) Depends on gradient of beam, index of refraction of bead vs. water. ...
Aberration-free three-dimensional multiphoton imaging of neuronal
Aberration-free three-dimensional multiphoton imaging of neuronal

Lasers versus LEDs for Bioinstrumentation Laser Advantage #1
Lasers versus LEDs for Bioinstrumentation Laser Advantage #1

Properties of a 4Pi confocal fluorescence microscope
Properties of a 4Pi confocal fluorescence microscope

P2SF: Physically-based Point Spread Function for
P2SF: Physically-based Point Spread Function for

... example, depth estimation [1] and image restoration [2]. On the other hand, this approach does not provide an accurate representation of aberrated optical system due to the lack of physical foundation and the limited parametric description of the PSF, which is based on simple functions , such as pil ...
Microscope
Microscope

Fraunhofer Diffraction
Fraunhofer Diffraction

J. Spigulis. Side-emitting optical fibers brighten our world in new
J. Spigulis. Side-emitting optical fibers brighten our world in new

Deconvolution Microscopy
Deconvolution Microscopy

... The greatest limitation of optical microscopy is spatial resolution, which is in the range of the wavelength of light used. The ultimate goal of cell microscopy is to capture the activity of cell components. In this respect, the resolution of limited numerical aperture wide-field microscopy may not ...
Fast Optical Communication Components
Fast Optical Communication Components

... An optical fiber is a cylindrical dielectric waveguide that transmits light along its axis, by the process of total internal reflection. The fiber consists of a core surrounded by a cladding layer. To confine the optical signal in the core, the refractive index of the core must be greater than that ...
Optical Filters for Laser-based Fluorescence Microscopes
Optical Filters for Laser-based Fluorescence Microscopes

... Optical filters play a vital role in obtaining maximum performance from complex, expensive, laser-based microscopes and it only makes sense to invest in optical filters that match the performance of the imaging system. What about the future of laser-based imaging systems? In order to gain a better i ...
laser1
laser1

... • A state in which a substance has been energized, or excited to specific energy levels. • More atoms or molecules are in a higher excited state. • The process of producing a population inversion is called pumping. • Examples: →by lamps of appropriate intensity →by electrical discharge ...


... optical CT scanning for gel dosimetry Optical CT scanning is a convenient, bench-top method of imaging 3D dose distributions in gel dosimeters such as BANG®. These scanners offer medical physicists a powerful tool for treatment plan validation in IMRT and conformal RTP. However, one of the limitatio ...
Increasing the Resolution of Far
Increasing the Resolution of Far

... numerical aperture, NA. To obtain a high resolution, a short wavelength and a high numerical aperture are desirable. However, the use of short wavelengths and high apertures is technically limited since the shortest usable wavelength λ is around 350 nm and the highest available numerical aperture is ...
Synopsis by Shawn Miller
Synopsis by Shawn Miller

Journal of Applied Science and Agriculture
Journal of Applied Science and Agriculture

2011 Research Poster
2011 Research Poster

Light Field Microscopy - Stanford Computer Graphics Laboratory
Light Field Microscopy - Stanford Computer Graphics Laboratory

Slide 1
Slide 1

supporting material
supporting material

Measurement of Surface Quality 1. Lyot Test 2. FECO 3. Nomarski
Measurement of Surface Quality 1. Lyot Test 2. FECO 3. Nomarski

... surface. The point separation (shear at the test surface) is usually comparable to the optical resolution of the microscope objective and hence only one image is seen. The image shows slope changes and it appears as though the surface has been illuminated from one side. Like a shearing interferomete ...
< 1 ... 46 47 48 49 50 51 52 53 54 ... 61 >

Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report