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Multiple wavelength diffractive imaging - X
Multiple wavelength diffractive imaging - X

... Fig. 4!c", showing excellent agreement with the image in Fig. 4!a". The MEM refinement produces a much smoother image but some of the detail in the sample is lost, indicating that the algorithm is probably underfitting the data due to an overestimate of the noise level. The faint crosslike structure ...
Note
Note

Super-Resolution Fluorescence Microscopy by Structured Light
Super-Resolution Fluorescence Microscopy by Structured Light

... a camera. ...
Direct detection of acoustic waves by laser light diffraction and
Direct detection of acoustic waves by laser light diffraction and

... of this study is to establish the optical technique to measure the extremely long wavelength, which has not ever been treated. In the present study, the Fraunhofer diffraction method, which was developed as a new means to detect the electromagnetic radiation scattered within the penetrating laser be ...
LIGHT APLIFICATION by STIMULATED EMISSION of RADITIONS
LIGHT APLIFICATION by STIMULATED EMISSION of RADITIONS

Optimization of axial resolution in a confocal microscope with
Optimization of axial resolution in a confocal microscope with

Focusing of light through a stratified medium: a practical
Focusing of light through a stratified medium: a practical

Near-field optical micromanipulation
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... 1.8 W / (2 x 10-4m)2 0.005 W / (4 x 10-6m)2 ...
Particles and Waves in Electron Optics and Microscopy, Vol 194.... Imaging and Electron Physics
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Jannick Rolland, PhD

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AGS1927 Technical Datasheet

3D differential interference contrast microscopy using synthetic
3D differential interference contrast microscopy using synthetic

... Differential interference contrast microscopy techniques visualize a specimen of weak contrast by converting the field gradient of a light into intensity variation. It has served as a label-free tool to image biological specimens.1,2 However, the intensity in the DIC image is determined by the coupl ...
Activity: Emission spectroscopy and smart sensors
Activity: Emission spectroscopy and smart sensors

Click To
Click To

... Patent No. 7,639,722 ...
Wollaston and Nomarski Prisms
Wollaston and Nomarski Prisms

... Birefringent Wollaston and/or Nomarski prisms are inserted in the optical pathway with their shear axis oriented at a 45-degree angle (northwest to southeast) to the polarizer and analyzer. The prisms are composed of two precisely ground and polished wedge-shaped slabs produced from high-grade optic ...
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Module Descriptor - What is FlexiLearn?

... This module covers the fundamentals of optic al design and image formation. The course will provide graduate students using optical systems with an in-depth knowledge of optical design. In particular, the course focuses on:  Basic Concepts of Geometrical Optics  Basic concepts of image formation  ...
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Optical Molasses

Problem Set 1 (due 2/21/06)
Problem Set 1 (due 2/21/06)

... 2. How do you collect a fluorescence emission spectrum? A fluorescence excitation spectrum? An absorption spectrum? Which two most closely resemble each other? A fluorescence emission spectrum is collected by illuminating the sample with a single wavelength and collecting a scan of the intensities o ...
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Optical microscopy laboratory practice 2012

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Ref_Note_final092911

... An additional characteristic of X-PEEM is the high surface sensitivity that is inherent with the detection of secondary electrons. In most materials, the low kinetic energy of the secondaries (typically <10 eV) limits the electron escape depth to 2 – 5 nm. This can be an advantage in some cases, how ...
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Advantages of Infinity-Corrected Optics in FT

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Slow light in room-temperature optical waveguides

UV-light microscope: improvements in optical imaging for a
UV-light microscope: improvements in optical imaging for a

Miniaturized modules for light sheet microscopy with low chromatic
Miniaturized modules for light sheet microscopy with low chromatic

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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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