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Lab 11: Atomic Spectra
Lab 11: Atomic Spectra

... Fluorescence occurs when an atom absorbs light at one frequency but then emits light at lower frequencies. For example, an atom can absorb a UV photon and jump to a higher energy state. Rather then jumping directly back to the initial energy state (and emitting the same energy UV photon), the atom c ...
Microscopes:
Microscopes:

Three-dimensional imaging by optical sectioning in the aberration
Three-dimensional imaging by optical sectioning in the aberration

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...  At the image plane, we now have two types of high-frequency fields, phase shifted by /2 and not, which generate high contrast fringes (bright or dark halos, depending on the sign of the phase shift filter). The existence of the halo indicates the failure of our assumption for a perfect 2D Fourie ...
Imaging properties of a metamaterial superlens
Imaging properties of a metamaterial superlens

Live Data Mode
Live Data Mode

Equipment list: Description Supplier Model Optical test bench
Equipment list: Description Supplier Model Optical test bench

... instrument can perfectly align each optical component’s center-of-curvature and position on its on-axis focused beam, exactly to specifications. The PSM is also used for aligning aspheric optics, including off-axis aspheres. The PSM locates point images and shows the image shape as a star test. The ...
Noniterative Exact Solution to the Phase Problem in Optical Imaging Implemented with Scanning Probe Microscope.
Noniterative Exact Solution to the Phase Problem in Optical Imaging Implemented with Scanning Probe Microscope.

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PDF

Fluorescence
Fluorescence

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Microscopic Analysis

Prof. Lan Yang - Microlasers for Nanoscale
Prof. Lan Yang - Microlasers for Nanoscale

Dual-color total internal reflection fluorescence cross
Dual-color total internal reflection fluorescence cross

... excitation and single-photon detection. Nonetheless, for several applications, TIR illumination can provide further benefits, for instance when ligands interacting with receptors immobilized onto a surface are to be studied.9–12 First, in TIRFCCS the molecules are only excited near the glass-sample ...
A1982PU06800001
A1982PU06800001

Fiber Optic Light Sources - Electrical and Computer
Fiber Optic Light Sources - Electrical and Computer

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Click To

AcuityXR Technology Significantly Enhances Lateral Resolution of
AcuityXR Technology Significantly Enhances Lateral Resolution of

AC-256 T(610-690) - Frankfurt Laser Company
AC-256 T(610-690) - Frankfurt Laser Company

The Fabry-Perot Cavity
The Fabry-Perot Cavity

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Resolution in Confocal Microscopy
Resolution in Confocal Microscopy

... The FWHM value is only one method of measuring resolution. Another common method is the Rayleigh criteria [19] where we would consider the distance between the first diffraction minimum and the central maximum as being the resolution of the optical signal. Note that although there FWHM might be in t ...
Toward the development of a Three-Dimensional Mid–Field Microscope
Toward the development of a Three-Dimensional Mid–Field Microscope

... Recently, an extraordinary transmission of light through small holes (<200 nm) in a thin metallic film has been described. This phenomenon has been shown to be the result of the photon-plasmon interaction in thin films where a periodic structure (such as a set of holes) is embedded in the film. One ...
Confocal microscopy with a volume holographic filter
Confocal microscopy with a volume holographic filter

... and improved aberration performance: Objectivelens aberrations are phase conjugated out during the hologram reconstruction process, and collector-lens aberrations (which increase the collected spot size) are irrelevant in the absence of a pinhole. The volume holographic confocal microscope is shown ...
Abstract
Abstract

Instrumental Analysis as Applied to Architectural Materials
Instrumental Analysis as Applied to Architectural Materials

... The light may be either reflected by the sample or transmitted through the sample. The image generated is magnified through lenses. Optical microscopy is used to analyze coatings, stones, and mortar and to identify constituents such as minerals (crystals). Microscopy is typically qualitative but may ...
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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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