bright field microscopy
... • A special condenser lens is used to illuminate the specimen diagonally, then observe light scattering off it. • The field of view is darker than bright field microscopy because illumination light does not enter the objective lens. • Oblique illumination is used to increase the visibility of specim ...
... • A special condenser lens is used to illuminate the specimen diagonally, then observe light scattering off it. • The field of view is darker than bright field microscopy because illumination light does not enter the objective lens. • Oblique illumination is used to increase the visibility of specim ...
MICROSCOPY
... more advanced) technique employs focal plane array detection for infrared chemical imaging, where the image contrast is determined by the response of individual sample regions to particular IR wavelengths selected by the user. ...
... more advanced) technique employs focal plane array detection for infrared chemical imaging, where the image contrast is determined by the response of individual sample regions to particular IR wavelengths selected by the user. ...
High resolution spectral self-interference fluorescence microscopy
... biological specimens. Light would be an ideal carrier of microscopic information if it weren’t for the resolution limit. But because of diffraction, standard light microscopes are not able to discern two objects closer together than about 200 nm in the focal plane. In the direction perpendicular to ...
... biological specimens. Light would be an ideal carrier of microscopic information if it weren’t for the resolution limit. But because of diffraction, standard light microscopes are not able to discern two objects closer together than about 200 nm in the focal plane. In the direction perpendicular to ...
Biology 177: Principles of Modern Microscopy
... is the refractive index of the intervening medium, FD (l) is the fluorescence emission intensity at a given wavelength l (in cm) eA (l) is the extinction coefficient of the acceptor (in cm -1 M -1). The orientation factor k2 can vary between 0 and 4, but ...
... is the refractive index of the intervening medium, FD (l) is the fluorescence emission intensity at a given wavelength l (in cm) eA (l) is the extinction coefficient of the acceptor (in cm -1 M -1). The orientation factor k2 can vary between 0 and 4, but ...
Lecture 1. Introduction. Nature of light, geometric optics.
... • S is distance from the object; S’ is distance from the image • Sign conventions: m = positive for inverted image; negative for upright • Sign conventions: f = positive for converging lens; negative for diverging ...
... • S is distance from the object; S’ is distance from the image • Sign conventions: m = positive for inverted image; negative for upright • Sign conventions: f = positive for converging lens; negative for diverging ...
Lecture 18
... Modulation transfer function • Resolution and performance of optical microscope can be characterized by the modulation transfer function (MTF) • MTF is measurement of microscope's ability to transfer contrast from the specimen to the image plane at specific resolution. • Incorporates resolution and ...
... Modulation transfer function • Resolution and performance of optical microscope can be characterized by the modulation transfer function (MTF) • MTF is measurement of microscope's ability to transfer contrast from the specimen to the image plane at specific resolution. • Incorporates resolution and ...
super-resolved fluorescence microscopy
... principle can also be implemented by Saturated Structural Illumination Microscopy (SSIM) (Gustafsson, 2005). These methods can be used when the fluorescing regions contain ensembles of fluorophores as well as single fluorophores and are therefore generically referred to as “Super-resolved ensemble f ...
... principle can also be implemented by Saturated Structural Illumination Microscopy (SSIM) (Gustafsson, 2005). These methods can be used when the fluorescing regions contain ensembles of fluorophores as well as single fluorophores and are therefore generically referred to as “Super-resolved ensemble f ...
Measurement of the 4Pi-confocal point spread function proves 75
... Cambridge, England). The bead was scanned 1.5 pm along the optical axis and 0.8 pm in lateral direction. The smallest resolvable step of the stage was 10 nm, thus providing a high-precision measurement of the PSF. The confocal resolution was determined by using the 4Pi-confocal arrangement with the ...
... Cambridge, England). The bead was scanned 1.5 pm along the optical axis and 0.8 pm in lateral direction. The smallest resolvable step of the stage was 10 nm, thus providing a high-precision measurement of the PSF. The confocal resolution was determined by using the 4Pi-confocal arrangement with the ...
Prof. Knut W. Urban
... - An expedition into the world of atoms by aberration-corrected electron optics The realization of aberration-corrected lenses has triggered a quantum jump in electron optics. The recent generation of transmission electron microscopes with aberration-corrected optics allows materials science in atom ...
... - An expedition into the world of atoms by aberration-corrected electron optics The realization of aberration-corrected lenses has triggered a quantum jump in electron optics. The recent generation of transmission electron microscopes with aberration-corrected optics allows materials science in atom ...
Compensated lens-free light field microscopy
... Lens-free light field microscopy (LLFM) is a holographic microscope with large field of view which produces an image of micro-range resolution using an interferometric reconstruction method [1]. The quality of LLFM imaging is limited due to the effect of image degradation factors such as (1) optical ...
... Lens-free light field microscopy (LLFM) is a holographic microscope with large field of view which produces an image of micro-range resolution using an interferometric reconstruction method [1]. The quality of LLFM imaging is limited due to the effect of image degradation factors such as (1) optical ...