Supplementary Tables and Figures Legends (doc 39K)
... ciprofloxacin, daunorubicin, kanamycin and paroxetine used to identify frequent hitters in K562, KU812 and chronic phase CML patient cells. Amino groups employed to immobilize each drug on the Sepharose beads (Rix, 2007) are shown in blue. The five compounds, being drugs themselves, were chosen for ...
... ciprofloxacin, daunorubicin, kanamycin and paroxetine used to identify frequent hitters in K562, KU812 and chronic phase CML patient cells. Amino groups employed to immobilize each drug on the Sepharose beads (Rix, 2007) are shown in blue. The five compounds, being drugs themselves, were chosen for ...
Multipower Sportsfood launches Fit Protein Lite
... Fit Protein Lite delivers 80% less carbs and sugars than Multipower’s number one selling Fit Protein in the iconic brown bottle. Retailing at just £3.85 a bottle the 500ml drink is available in three delicious flavours of Chocolate, Vanilla and Strawberry. Multipower Nutritionist Drew Price said: “F ...
... Fit Protein Lite delivers 80% less carbs and sugars than Multipower’s number one selling Fit Protein in the iconic brown bottle. Retailing at just £3.85 a bottle the 500ml drink is available in three delicious flavours of Chocolate, Vanilla and Strawberry. Multipower Nutritionist Drew Price said: “F ...
The Scientist : Lab Tools: Close Encounters
... The problem: Y2H measures only binary interactions, not multiprotein complexes. But coIP /MS requires high-quality, specific antibodies, few of which are available. Superti-Furga needed a generic way to isolate intact complexes from cells. The Solution: Superti-Furga and his team adapted a process c ...
... The problem: Y2H measures only binary interactions, not multiprotein complexes. But coIP /MS requires high-quality, specific antibodies, few of which are available. Superti-Furga needed a generic way to isolate intact complexes from cells. The Solution: Superti-Furga and his team adapted a process c ...
ProteomeLab™ XL-A/XL-I Protein
... reformulations. Aggregation, which affects bioactivity, pharmacokinetics, and immunogenicity is of particular interest in the area of protein therapeutics. High dose protein drugs, such as monoclonal antibodies administered subcutaneously, require formulations at high concentration (>50 mg/mL) which ...
... reformulations. Aggregation, which affects bioactivity, pharmacokinetics, and immunogenicity is of particular interest in the area of protein therapeutics. High dose protein drugs, such as monoclonal antibodies administered subcutaneously, require formulations at high concentration (>50 mg/mL) which ...
Prediction of protein disorder: basic concepts and practical hints
... Disordered protein complexes • Interaction sites are usually linear ...
... Disordered protein complexes • Interaction sites are usually linear ...
Antibody Production and Use in Immunodetection
... for an antibody molecule or the binding portion of an antibody molecule is inserted in bacteria,yeast or human cells The expressed antibody is pulled from the cells No animal is used ...
... for an antibody molecule or the binding portion of an antibody molecule is inserted in bacteria,yeast or human cells The expressed antibody is pulled from the cells No animal is used ...
A proteomic platform that can also detect SNPs
... uracil greatly expand the physicochemical diversity of the large combinatorial SELEX libraries from which they are selected, resulting in binding molecules to more proteins, and with higher affinity, than observed with traditional aptamers. The hydrophobic nature of these interactions results in exq ...
... uracil greatly expand the physicochemical diversity of the large combinatorial SELEX libraries from which they are selected, resulting in binding molecules to more proteins, and with higher affinity, than observed with traditional aptamers. The hydrophobic nature of these interactions results in exq ...
PowerPoint 簡報 - Academia Sinica
... • The higher immunogenicity of KLH often makes it the preferred choice. Another advantage of choosing KLH over BSA is that BSA is used as a blocking agent in many experimental assays. Because antisera raised against peptides conjugated to BSA will also contain antibodies to BSA, false positives may ...
... • The higher immunogenicity of KLH often makes it the preferred choice. Another advantage of choosing KLH over BSA is that BSA is used as a blocking agent in many experimental assays. Because antisera raised against peptides conjugated to BSA will also contain antibodies to BSA, false positives may ...
One of the best ways to get the full benefit of your supplements
... cannot rely on food to provide us all the necessary nutrients and since there is no such thing as a universal supplement we need to stack more then one supplement to meet our goals. ...
... cannot rely on food to provide us all the necessary nutrients and since there is no such thing as a universal supplement we need to stack more then one supplement to meet our goals. ...
Protein Origami
... living things. They carry oxygen in the blood, break down glucose in the body to release energy, capture sunlight to feed growing plants, catalyze the chemical reaction that makes fireflies glow, and a lot more. Proteins also aid viruses in invading cells. AIDS can be tied to proteins that break thr ...
... living things. They carry oxygen in the blood, break down glucose in the body to release energy, capture sunlight to feed growing plants, catalyze the chemical reaction that makes fireflies glow, and a lot more. Proteins also aid viruses in invading cells. AIDS can be tied to proteins that break thr ...
MONOHYBRID CROSS
... It is a kind of variation related to biodiversity, genetic variation, and adaptation Presence of more than one genetically distinct type in a single population Useful tools in genetic studies for linkage analysis, prenatal diagnosis, criminal cases and paternity ...
... It is a kind of variation related to biodiversity, genetic variation, and adaptation Presence of more than one genetically distinct type in a single population Useful tools in genetic studies for linkage analysis, prenatal diagnosis, criminal cases and paternity ...
Mihaela_Leonida_Abstract
... Although the use of proteins and peptides as therapeutic agents and as antimicrobials in the food industry has expanded, problems are still encountered due to their susceptibility to proteolytic degradation, low solubility, and often physicochemical instability. As a solution to these problems, we p ...
... Although the use of proteins and peptides as therapeutic agents and as antimicrobials in the food industry has expanded, problems are still encountered due to their susceptibility to proteolytic degradation, low solubility, and often physicochemical instability. As a solution to these problems, we p ...
protein-protein interactions
... technology: systematic affinity purification followed by mass spectrometry ...
... technology: systematic affinity purification followed by mass spectrometry ...
Functional Assay for Botulinum Neurotoxin Type A
... (#2102). GST-human SV2c (125 µg) was added to the protein A resin with AntiGST antibodies and incubated overnight at 4ºC. The bound resin was washed and 100µl was aliquoted into each spin column. Dilutions of BTA were added to each column and incubated for 4 hours at room temperature. Unbound toxin ...
... (#2102). GST-human SV2c (125 µg) was added to the protein A resin with AntiGST antibodies and incubated overnight at 4ºC. The bound resin was washed and 100µl was aliquoted into each spin column. Dilutions of BTA were added to each column and incubated for 4 hours at room temperature. Unbound toxin ...
Section 3A Analysis on a Western Blot
... antibody reagents. To be sure the procedure works optimally for a given tagged protein, the optimal concentration of antibody must always be determined experimentally. If you are working with c-myc-, HA-, or VSV-G tagged proteins and BM tag-specific antibody reagents, the antibody concentrations and ...
... antibody reagents. To be sure the procedure works optimally for a given tagged protein, the optimal concentration of antibody must always be determined experimentally. If you are working with c-myc-, HA-, or VSV-G tagged proteins and BM tag-specific antibody reagents, the antibody concentrations and ...
Detection of protein-protein interactions
... • Expression of target and bait protein fusions are induced by IPTG. • If bait and target interact, RNApol initiates transcription of His3 and Strepr genes. • Screening 1st for His prototrophy, 2nd for streptomycin resistance. ...
... • Expression of target and bait protein fusions are induced by IPTG. • If bait and target interact, RNApol initiates transcription of His3 and Strepr genes. • Screening 1st for His prototrophy, 2nd for streptomycin resistance. ...
Denaturation of proteins
... Denaturation of proteins. The structure/function principle we covered in class tells us that changing the structure of a protein will affect its function. Often that means that function is lost. Denaturation of a protein means loss of the protein's function due to structural change in the protein ca ...
... Denaturation of proteins. The structure/function principle we covered in class tells us that changing the structure of a protein will affect its function. Often that means that function is lost. Denaturation of a protein means loss of the protein's function due to structural change in the protein ca ...
Immunoglobulin
... Bonds between two amino acids result of the SH (sulfhydral) group of one amino acid covalently bonding to the SH group of another amino acid Stronger than hydrogen bonds Eg. Hair proteins are held together by disulfide bonds ...
... Bonds between two amino acids result of the SH (sulfhydral) group of one amino acid covalently bonding to the SH group of another amino acid Stronger than hydrogen bonds Eg. Hair proteins are held together by disulfide bonds ...
domains
... Polypeptide subunits associate in a geometrically specific manner. Why subunits? Easier to repair self-assembling single subunit vs. a large polypeptide. • Increasing a protein’s size through subunits is more efficient for specifying the active site. • Provides a structural basis for regulating acti ...
... Polypeptide subunits associate in a geometrically specific manner. Why subunits? Easier to repair self-assembling single subunit vs. a large polypeptide. • Increasing a protein’s size through subunits is more efficient for specifying the active site. • Provides a structural basis for regulating acti ...
TUTORIAL 5 Multiple Choices For each of the questions below
... TUTORIAL 5 Multiple Choices For each of the questions below select the one best answer. ...
... TUTORIAL 5 Multiple Choices For each of the questions below select the one best answer. ...
NMR - University of Puget Sound
... Sidechain location vs. polarity -Nonpolar residues in interior of protein (hydrophobic effect promotes this, as well as efficient packing of those sidechains) -Charged polar residues on protein surface (immersing charge in anhydrous interior is energetically unfavorable) -Uncharged polar groups occu ...
... Sidechain location vs. polarity -Nonpolar residues in interior of protein (hydrophobic effect promotes this, as well as efficient packing of those sidechains) -Charged polar residues on protein surface (immersing charge in anhydrous interior is energetically unfavorable) -Uncharged polar groups occu ...
Protein 101 A3 poster.indd
... Below is a list of common highprotein foods, with the amount of protein per 100g or per unit included: ...
... Below is a list of common highprotein foods, with the amount of protein per 100g or per unit included: ...