Advanced in Antibody Design
... Molecular architectures of bispecific monoclonal antibodies (mAbs). Two mAbs are recombined into different bispecific architectures. A quadroma Triomab (Trion Pharma, Munich, Germany) comprises one heavy chain–light chain pair of a rat immunoglobulin G2 (IgG2) and one heavy chain–light chain pair o ...
... Molecular architectures of bispecific monoclonal antibodies (mAbs). Two mAbs are recombined into different bispecific architectures. A quadroma Triomab (Trion Pharma, Munich, Germany) comprises one heavy chain–light chain pair of a rat immunoglobulin G2 (IgG2) and one heavy chain–light chain pair o ...
Advanced in Antibody Design
... Molecular architectures of bispecific monoclonal antibodies (mAbs). Two mAbs are recombined into different bispecific architectures. A quadroma Triomab (Trion Pharma, Munich, Germany) comprises one heavy chain–light chain pair of a rat immunoglobulin G2 (IgG2) and one heavy chain–light chain pair o ...
... Molecular architectures of bispecific monoclonal antibodies (mAbs). Two mAbs are recombined into different bispecific architectures. A quadroma Triomab (Trion Pharma, Munich, Germany) comprises one heavy chain–light chain pair of a rat immunoglobulin G2 (IgG2) and one heavy chain–light chain pair o ...
RayBio Genomic DNA Magnetic Beads Kit
... 5. Centrifuge 10 minutes at 5,000 x g to pellet the cells, and discard the supernatant. 6. Add 200 µL Lysis Solution and vortex or pipette up and down to resuspend the pellet. 2. Incubate the sample at 55°C for 30 minutes (bacteria) to 1 hour (tissues) until digested, vortexing occasionally or using ...
... 5. Centrifuge 10 minutes at 5,000 x g to pellet the cells, and discard the supernatant. 6. Add 200 µL Lysis Solution and vortex or pipette up and down to resuspend the pellet. 2. Incubate the sample at 55°C for 30 minutes (bacteria) to 1 hour (tissues) until digested, vortexing occasionally or using ...
Annex I1/3 to the Certificate Νο 820
... anti-extractable nuclear antigens antibodies (ENA) (SS-A, SS-B, RNP, Sm), antiscleroderma-70 antibodies (antitopoisomerase I) (Scl-70), anti-cardiolipin antibodies (anti-phospholipid), anti-β2glycoprotein I antibodies (anti-β2GPI), antimitochondrial antibodies (AMA), antithyroid microsomal antibodie ...
... anti-extractable nuclear antigens antibodies (ENA) (SS-A, SS-B, RNP, Sm), antiscleroderma-70 antibodies (antitopoisomerase I) (Scl-70), anti-cardiolipin antibodies (anti-phospholipid), anti-β2glycoprotein I antibodies (anti-β2GPI), antimitochondrial antibodies (AMA), antithyroid microsomal antibodie ...
Macromolecular Interactions
... to identify the consensus sequence—each cycle identifies the N-terminal residue ...
... to identify the consensus sequence—each cycle identifies the N-terminal residue ...
Potassium sulfate Product Number P0772 Store at - Sigma
... 3. Lehrer, S. S., and Yuan, A., The stability of tropomyosin at acid pH: effects of anion binding. J. Struct. Biol., 122(1-2), 176-179 (1998). 4. Lee, K. K., et al., Electrostatic effects in highly charged proteins: salt sensitivity of pK a values of histidines in staphylococcal nuclease. Biochemist ...
... 3. Lehrer, S. S., and Yuan, A., The stability of tropomyosin at acid pH: effects of anion binding. J. Struct. Biol., 122(1-2), 176-179 (1998). 4. Lee, K. K., et al., Electrostatic effects in highly charged proteins: salt sensitivity of pK a values of histidines in staphylococcal nuclease. Biochemist ...
RISE-Workshop
... The purpose of the stacking gel is to concentrate the protein sample into a sharp band before it enters the mail separating gel, thus giving sharper protein bands in the separating gel. This modification allows relatively larger sample volumes to be applied to the gel without any loss of resolution. ...
... The purpose of the stacking gel is to concentrate the protein sample into a sharp band before it enters the mail separating gel, thus giving sharper protein bands in the separating gel. This modification allows relatively larger sample volumes to be applied to the gel without any loss of resolution. ...
FlexWeb
... 3. Many biochemical process are regulated by protein switching from one shape to another shape. ...
... 3. Many biochemical process are regulated by protein switching from one shape to another shape. ...
The Protein Interaction Prediction Engine (PIPE)
... The In Silico Protein Synthesizer (InSiPS) is a computational tool that can synthesize proteins with specific protein-protein interaction prediction profiles. Given a set of target proteins and a set of non-target proteins, InSiPS can generate a protein sequence that is predicted to interact with th ...
... The In Silico Protein Synthesizer (InSiPS) is a computational tool that can synthesize proteins with specific protein-protein interaction prediction profiles. Given a set of target proteins and a set of non-target proteins, InSiPS can generate a protein sequence that is predicted to interact with th ...
Protein Concentration Determination In nearly any biochemistry
... UV absorbance: Protein concentrations can be determined directly by ultraviolet spectroscopy because of the presence of tyrosine and tryptophan which absorb at 280 nm. Because the levels of these two amino acids vary greatly from protein to protein, the UV absorbance per milligram protein is highly ...
... UV absorbance: Protein concentrations can be determined directly by ultraviolet spectroscopy because of the presence of tyrosine and tryptophan which absorb at 280 nm. Because the levels of these two amino acids vary greatly from protein to protein, the UV absorbance per milligram protein is highly ...
S1 Text.
... The sample was again purified with AMPure XP beads and eluted in 52 µL elution buffer (Qiagen). 4 µL of this product was used in a preliminary PCR amplification with 12.5 µL Phusion High-Fidelity Master Mix (NEB), 1 µL of each 10 µM modified Solexa© Amplification primer mix (2006 Illumina, Inc.; P1- ...
... The sample was again purified with AMPure XP beads and eluted in 52 µL elution buffer (Qiagen). 4 µL of this product was used in a preliminary PCR amplification with 12.5 µL Phusion High-Fidelity Master Mix (NEB), 1 µL of each 10 µM modified Solexa© Amplification primer mix (2006 Illumina, Inc.; P1- ...
2016-10-12 Jurgen Chemical Proteomics
... spanning molecular and cell biology, biochemistry, pharmacology, analytical and organic chemistry, physics, informatics,… … aims to study how small molecules (“chemicals”) of synthetic or natural origin bind to proteins and modulate their function. … can be applied in drug target discovery or to ide ...
... spanning molecular and cell biology, biochemistry, pharmacology, analytical and organic chemistry, physics, informatics,… … aims to study how small molecules (“chemicals”) of synthetic or natural origin bind to proteins and modulate their function. … can be applied in drug target discovery or to ide ...
NIDA-svisit-20071219-PARE - Yale Bioinformatics -
... that mRNA expression levels should be correlated with protein abundance …Among pathways, this is expected to a lesser degree between interacting proteins ...
... that mRNA expression levels should be correlated with protein abundance …Among pathways, this is expected to a lesser degree between interacting proteins ...
CHAPTER 4 Proteins: Structure, Function, Folding
... • Conformational changes may occur upon ligand binding (Daniel Koshland in 1958) – This adaptation is called the induced fit – Induced fit allows for tighter binding of the ligand – Induced fit allows for high affinity for different ligands • Both the ligand and the protein can change their conforma ...
... • Conformational changes may occur upon ligand binding (Daniel Koshland in 1958) – This adaptation is called the induced fit – Induced fit allows for tighter binding of the ligand – Induced fit allows for high affinity for different ligands • Both the ligand and the protein can change their conforma ...
Holliday.EPO.Claim.Types.Antibodies
... given compound…would fall within the scope of what is claimed. It would require undue experimentation (be an undue burden) to randomly screen undefined compounds for the claimed activity” ...
... given compound…would fall within the scope of what is claimed. It would require undue experimentation (be an undue burden) to randomly screen undefined compounds for the claimed activity” ...
immobilised metal affinity chromatography (imac) beads for
... obtained by several techniques such as ion exchange chromatography, ultrafiltration and affinity chromatography. However rigorous limitations occur including lengthy steps, complicated and expensive for large scale production has hampered the applications. Separation technique for protein by Immobil ...
... obtained by several techniques such as ion exchange chromatography, ultrafiltration and affinity chromatography. However rigorous limitations occur including lengthy steps, complicated and expensive for large scale production has hampered the applications. Separation technique for protein by Immobil ...
Supplementary information
... with an elution buffer (1% SDS, 0.1 M NaHCO3). DNA-protein cross-links were reversed at 65C overnight, and then incubated with RNase and proteinase K. DNA was purified using phenol/chloroform and ethanol precipitation as templates for qRT-PCR. The 5 upstream region of BrZ7 was cloned using the gen ...
... with an elution buffer (1% SDS, 0.1 M NaHCO3). DNA-protein cross-links were reversed at 65C overnight, and then incubated with RNase and proteinase K. DNA was purified using phenol/chloroform and ethanol precipitation as templates for qRT-PCR. The 5 upstream region of BrZ7 was cloned using the gen ...
Enzyme Linked Immunosorbent Assay (ELISA)
... result. • Unknowns that generate a signal that is stronger than the known sample are called "positive"; those that generate weaker signal are called "negative. ...
... result. • Unknowns that generate a signal that is stronger than the known sample are called "positive"; those that generate weaker signal are called "negative. ...
Introduction Quality Protein Maize (QPM) contains nearly twice as
... Introduction Quality Protein Maize (QPM) contains nearly twice as much usable protein as other maize grown in Kenya. The majority of people in Kenya depend on maize as their principal daily food, for weaning babies, and for feeding livestock. Unfortunately normal maize has one significant flaw, it l ...
... Introduction Quality Protein Maize (QPM) contains nearly twice as much usable protein as other maize grown in Kenya. The majority of people in Kenya depend on maize as their principal daily food, for weaning babies, and for feeding livestock. Unfortunately normal maize has one significant flaw, it l ...
elisa plates - immunology.unideb.hu
... The isotypes of these antibodies additionally could refer the fresh/persistent (IgM dominance) or repeated/memory immune response (IgG dominance) against the parasite. The possibilities can be discriminated by the use of isotype specific secondary antibodies. ...
... The isotypes of these antibodies additionally could refer the fresh/persistent (IgM dominance) or repeated/memory immune response (IgG dominance) against the parasite. The possibilities can be discriminated by the use of isotype specific secondary antibodies. ...
北京聚合美生物科技有限公司 Mei5 Biotechnology, Co., Ltd M5 GFP
... Store at -20°C. Stable for one year from the date of shipment. 【BACKGROUND】 The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, with ...
... Store at -20°C. Stable for one year from the date of shipment. 【BACKGROUND】 The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, with ...
Enzyme-linked immunosorbent assay (ELISA)
... different epitopes on each antigen are present. Such a response is described as polyclonal, as antibody is derived from more than one clone of B lymphocytes and shows heterogeneity in the amino acid sequences of the antigenbinding immunoglobulins present. However, more recently, methods have been de ...
... different epitopes on each antigen are present. Such a response is described as polyclonal, as antibody is derived from more than one clone of B lymphocytes and shows heterogeneity in the amino acid sequences of the antigenbinding immunoglobulins present. However, more recently, methods have been de ...
Discrepancy between use of lean body mass or nitrogen balance to
... defined as having nitrogen intake equal nitrogen losses (mainly in urine and feces). The question has arisen whether nitrogen balance is an adequate measurement of protein requirements or whether measurement of lean body mass is a better option. Protein turnover refers to the continual catabolism an ...
... defined as having nitrogen intake equal nitrogen losses (mainly in urine and feces). The question has arisen whether nitrogen balance is an adequate measurement of protein requirements or whether measurement of lean body mass is a better option. Protein turnover refers to the continual catabolism an ...
100 Base Pair DNA Ladder
... ( 2 ) For best resolution of 100 bp to 1,500 bp, use 2.0% Metaphor agarose gel or equivalent agarose gel. For best resolution of 500 bp to 4,000 bp fragment, use 1.0% agarose gel. To facilitate assignment of bands above 1000 bp, it is helpful to run a 1kb DNA ladder in an adjacent lane. ( 3 ) To pin ...
... ( 2 ) For best resolution of 100 bp to 1,500 bp, use 2.0% Metaphor agarose gel or equivalent agarose gel. For best resolution of 500 bp to 4,000 bp fragment, use 1.0% agarose gel. To facilitate assignment of bands above 1000 bp, it is helpful to run a 1kb DNA ladder in an adjacent lane. ( 3 ) To pin ...