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Jasmita_presen_nov3
Jasmita_presen_nov3

... In silico screening Structure based virtual screening docking methods to fit putative ligands into 3D structure of target receptor ...
STSL – Specialized Translational Services Laboratory
STSL – Specialized Translational Services Laboratory

... on TMAs or whole tissue sections (WTS) using quantitative immunofluorescence (quantified by AQUA or the Vectra System) [4, 5]. Assay reproducibility is assessed with test TMAs (40 spots each), positive and negative controls are included. o   Digital Image Analysis and Quantification of DAB based IHC ...
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PPTX

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StarCellBio Exercise 2 – Orientation of Transmembrane Proteins
StarCellBio Exercise 2 – Orientation of Transmembrane Proteins

... Your graduate student advisor suggests you first determine the orientation of Protein X and Protein Y in the plasma membrane using western blotting, an experimental technique that allows for the detection of a specific protein or peptide after cells are lysed and proteins are isolated from the rest ...
Thermo Scientific Top Vision Low Melting Point Agarose
Thermo Scientific Top Vision Low Melting Point Agarose

... LIMITED USE LABEL LICENSE: Internal Research and Development Use Only. The purchase of this product conveys to the buyer the limited, non-exclusive, nontransferable right (without the right to resell, repackage, or further sublicense) to use this product for internal research and development purpos ...
Protein Purification Affinity purification
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... conditions. Rapid, one-step affinity purification results in active fusion proteins of highest purity. Physiological buffers like PBS in combination with a wide range of detergents, chelators, salt, and redox conditions can be used. The competitive elution with desthiobiotin, an inexpensive, reversi ...
Poster - Protein Information Resource
Poster - Protein Information Resource

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Custom Polyclonal Antibody Order Information

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Western Blot part 2_v2 - University of San Diego Home Pages
Western Blot part 2_v2 - University of San Diego Home Pages

... paper. This type of detection gives very sharp bands with low background staining of the membrane. This is one of the easiest methods for western blot detection. There are two disadvantages to this method: the signal or bands fade over time when exposed to light, and second, it is not as sensitive a ...
2004 Dot blotting presentation by Chng-Tau, Poppy, and
2004 Dot blotting presentation by Chng-Tau, Poppy, and

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Coomassie Blue R-250 (SureStain)

... used in place of the R-250 form to create a rapid and convenient staining procedure. This capability of G-250 is due to its particular properties. Coomassie G-250 manifests a leuco form below pH 2. Solutions of the dye, dark blue black at pH 7, turn a clear tan upon acidification. The leuco form rec ...
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p19 siRNA Binding Protein | NEB

... p19 siRNA Binding Protein contains no detectable DNases, RNases and phosphatases. The purified protein contains no detectable DNA or RNA as determined by ethidium bromide staining of an agarose gel. Quality Control Assays The following Quality Control Tests are performed on each new lot and meet the ...
REVERSE GENETICS: USING RNAi TO MAKE PROTEIN KNOCK
REVERSE GENETICS: USING RNAi TO MAKE PROTEIN KNOCK

... Using reverse genetics, one first identifies a gene of interest, and then determines what defect, if any, results when the corresponding protein is missing. This approach may be used to investigate whether a particular protein performs the same functions in one organism as a homologous protein (one ...
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proteomics - Sigma

... are hydrophilic, surface oriented, and flexible. Most naturally occurring proteins in aqueous solutions have their hydrophilic residues on the surface and their hydrophobic residues buried in the interior. Antibodies bind to epitopes on the surface of proteins. The N- and C-termini of proteins are o ...
Manual_AccuRapid™ Protein Synthesis Kit
Manual_AccuRapid™ Protein Synthesis Kit

... E. coli cell-free protein expression method is the coupled reaction of transcription and translation. The method use a mixture containing template DNA bearing a gene of interest (either expression vector or PCR product), E. coli cell extract and other required reagents such as amino acids and rNTPs. ...
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... Protein Dynamics and Relation to Function. Large-Scale Conformational Change. Ligand Binding and Macromolecular Association.  Drug Design ...
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1. dia - immunology.unideb.hu

... of prediabetic and Type 1 diabetic patients (including approximately 7-10% of adult onset diabetics with Type 1 diabetes) IA-2 (a tyrosine phosphatase-like protein) specific Ab. are found in 50-75% of Type 1 diabetic patients at and prior to disease onset, are generally more prevalent in younger pat ...
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... Under what conditions does the protein remain stable? How long does this stability continue? In the process of optimizing formulations, these and other questions call for an answer. Classical protein-biochemical methods use, for example, analytical size-exclusion chromatography to detect multimeric ...
Chapter 18: Applications of Immunology
Chapter 18: Applications of Immunology

... proportions as with immuno-precipitation • can use polyclonal or monoclonal antibody • allows detection of antibody to specific antigens as well as the determination of “antibody titer” by serial dilution ...
Use of Bacteria in Antibody Production - BLI-Research-Synbio
Use of Bacteria in Antibody Production - BLI-Research-Synbio

... • In addition, GFP will be made along with the antibody to demonstrate that the system is working ...
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Immunoprecipitation

Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.
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