Kay Hofmann - Tresch Group
... too much, the correcpt peptide can be identified by a good correlation of expected and observed pattern. Problems with polymorphisms, modifications, other unexpected things. ...
... too much, the correcpt peptide can be identified by a good correlation of expected and observed pattern. Problems with polymorphisms, modifications, other unexpected things. ...
Supplements - Maintenance
... Why use Physiquecal supplements? The quick answer is convenience, cost, and fastest way of replenishing nutrients. Physiquecal believe completely in the use of supplements. This has been from years of experience using different supplements from other leading brands. Physiquecal have invested time an ...
... Why use Physiquecal supplements? The quick answer is convenience, cost, and fastest way of replenishing nutrients. Physiquecal believe completely in the use of supplements. This has been from years of experience using different supplements from other leading brands. Physiquecal have invested time an ...
Rotation of Cells and Ion Exchange Beads in the MHz
... port this interpretation, ion exchange beads with different functional groups were investigated for their ability to rotate in an alternating electrical field. Most of the experiments were perform ed on Chelex beads (diam eter about 50 |im). Chelex beads are made up of polystyrene cross-linked with ...
... port this interpretation, ion exchange beads with different functional groups were investigated for their ability to rotate in an alternating electrical field. Most of the experiments were perform ed on Chelex beads (diam eter about 50 |im). Chelex beads are made up of polystyrene cross-linked with ...
elisa - WordPress.com
... (conjugated) to horseradish peroxidase (HRP) • Enzyme substrate: 3,3’,5,5’ – tetramethylbenzidine (TMB) – a colorless solution that when oxidized by HRP turns yellow. A substrate is a compound or substance that undergoes change. – Substrates bind to active sites on the surface of enzymes and are con ...
... (conjugated) to horseradish peroxidase (HRP) • Enzyme substrate: 3,3’,5,5’ – tetramethylbenzidine (TMB) – a colorless solution that when oxidized by HRP turns yellow. A substrate is a compound or substance that undergoes change. – Substrates bind to active sites on the surface of enzymes and are con ...
Chapter 5 Separations: I) Based on Charge or pI A) Electrophoresis
... B) Size exclusion chromatography Fine, porous beads (agarose, polyacrylamide) are packed into a chromatography column. The pore size of these beads approximates the dimensions of macromolecules. As a solution of macromolecules is passed through the column, the molecules distribute between the soluti ...
... B) Size exclusion chromatography Fine, porous beads (agarose, polyacrylamide) are packed into a chromatography column. The pore size of these beads approximates the dimensions of macromolecules. As a solution of macromolecules is passed through the column, the molecules distribute between the soluti ...
Electrophoresis
... Attach leads to tank and power supply; set V so I < 60 mA Agarose concentration needed is determined by sizes of DNA fragments to be separated o for fragments between 0.3 and 3 kb, 0.7-1% are good standard concentrations o to separate small fragments (0.2-0.7 kb) from one another, use 1.5-3% (small ...
... Attach leads to tank and power supply; set V so I < 60 mA Agarose concentration needed is determined by sizes of DNA fragments to be separated o for fragments between 0.3 and 3 kb, 0.7-1% are good standard concentrations o to separate small fragments (0.2-0.7 kb) from one another, use 1.5-3% (small ...
B Ca(2+)
... between experiments, the amount of bound protein was normalized to 12% of the total soluble protein used in the assay. Lipid binding. Tritiated liposomes were prepared from 12.5 mg of phosphatidylcholine and 5 mg of phosphatidylserine (Sigma, Poole, UK) with 20 µCi of 1,2-dipalmitoyl,L-3phosphatidyl ...
... between experiments, the amount of bound protein was normalized to 12% of the total soluble protein used in the assay. Lipid binding. Tritiated liposomes were prepared from 12.5 mg of phosphatidylcholine and 5 mg of phosphatidylserine (Sigma, Poole, UK) with 20 µCi of 1,2-dipalmitoyl,L-3phosphatidyl ...
Protein Separation and Purification
... E.g. Antibodies which bind Protein Enzyme which binds a co-enzyme or inhibitor A ligand is covalently bound to a solid matrix (usually agarose) which is then packed into a chromatography column When a mixture containing the protein of interest is applied to the column, the desired protein is bound b ...
... E.g. Antibodies which bind Protein Enzyme which binds a co-enzyme or inhibitor A ligand is covalently bound to a solid matrix (usually agarose) which is then packed into a chromatography column When a mixture containing the protein of interest is applied to the column, the desired protein is bound b ...
![Anti-PCB antibody [3H2AD9] ab110314 Product datasheet 3 Images Overview](http://s1.studyres.com/store/data/000076345_1-acbfa58e194757c519d151062b812354-300x300.png)
Anti-PCB antibody [3H2AD9] ab110314 Product datasheet 3 Images Overview
... Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) an ...
... Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) an ...
Chemicals
... Protein Identification by Mass Spectrometry MALDI-MS and MALDI-MS/MS were performed on an Applied Biosystems 4700 Proteomics Analyzer with TOF/TOF ion optics. Data were acquired in positive MS reflector mode with five spots of standard (ABI4700 Calibration Mixture) for calibration. Mass spectra were ...
... Protein Identification by Mass Spectrometry MALDI-MS and MALDI-MS/MS were performed on an Applied Biosystems 4700 Proteomics Analyzer with TOF/TOF ion optics. Data were acquired in positive MS reflector mode with five spots of standard (ABI4700 Calibration Mixture) for calibration. Mass spectra were ...
Department of Microbiology, Lab 016 instructions Standard gel
... Ethidiumbromide-solution: 10 mg EtBr/ml H2Odest Must be produced at least once a week. Store at RT. Note, Ethidiumbromide is hazardous! Ethidum bromide can be replaced with other nucleic acid stains like SYBR Green or SYBR Gold. ...
... Ethidiumbromide-solution: 10 mg EtBr/ml H2Odest Must be produced at least once a week. Store at RT. Note, Ethidiumbromide is hazardous! Ethidum bromide can be replaced with other nucleic acid stains like SYBR Green or SYBR Gold. ...
What Are the Best Food Sources of Protein
... What percent of your calorie intake should be made up of protein? What did you determine to be the actual amount of protein you should take in each day? ...
... What percent of your calorie intake should be made up of protein? What did you determine to be the actual amount of protein you should take in each day? ...
Protein PPT Editted
... Maintains acid-base balance in the blood Carries vital substances (combines with fat to form lipoproteins, transport of iron and other nutrients and oxygen in the blood Provides energy as a last resort if the body can’t get energy from carbs and fat or if there is too much protein in the diet Protei ...
... Maintains acid-base balance in the blood Carries vital substances (combines with fat to form lipoproteins, transport of iron and other nutrients and oxygen in the blood Provides energy as a last resort if the body can’t get energy from carbs and fat or if there is too much protein in the diet Protei ...
Abstract: Epitope-Enhanced Assay Sensitivity For Imaging
... iNP features a high intensity-fast decaying evanescent wave compared with traditional SPR and the total sensed volume around each nanostructure is below 100 nm. For assay towards proteins, the main bottleneck of the inhibition assay format for iNP technology is that the immobilization of a large mol ...
... iNP features a high intensity-fast decaying evanescent wave compared with traditional SPR and the total sensed volume around each nanostructure is below 100 nm. For assay towards proteins, the main bottleneck of the inhibition assay format for iNP technology is that the immobilization of a large mol ...
SURVEY OF BIOCHEMISTRY - Georgia Institute of Technology
... Lysine can form a salt bridge by associating with a nearby ____ residue. ...
... Lysine can form a salt bridge by associating with a nearby ____ residue. ...
Supplementary Information (doc 417K)
... using Taq polymerase and adapters were ligated. The DNA was loaded on gel and a band corresponding to ~300 bp (ChIP fragment + adapters) was collected. The DNA was isolated, amplified by PCR and used for cluster generation on the Illumina 1G or HiSeq genome analyzer. The 35-45 bp tags were mapped to ...
... using Taq polymerase and adapters were ligated. The DNA was loaded on gel and a band corresponding to ~300 bp (ChIP fragment + adapters) was collected. The DNA was isolated, amplified by PCR and used for cluster generation on the Illumina 1G or HiSeq genome analyzer. The 35-45 bp tags were mapped to ...
General Reference - Methods Enzymol. 182 "Guide to Protein
... x Thermal stability. 2). Isoelectric point and pH precipitation. x Proteins have their lowest solubility and greatest stability at the pH equal to their isoelectric point. x The pI of a protein is determined essentially by it's content of Asp + Glu vs His + Lys + Arg. For a protein with 3 Asp, 3 Glu ...
... x Thermal stability. 2). Isoelectric point and pH precipitation. x Proteins have their lowest solubility and greatest stability at the pH equal to their isoelectric point. x The pI of a protein is determined essentially by it's content of Asp + Glu vs His + Lys + Arg. For a protein with 3 Asp, 3 Glu ...
JustWhey_Flyer_Layout 1
... SportPharma Just-WHEY uses the finest quality, non-denatured, specially filtered whey proteins on the market and is designed to help boost your daily protein intake. What’s so great about protein? Well, protein consists of amino acids, which are the building blocks for muscle tissue. Among the criti ...
... SportPharma Just-WHEY uses the finest quality, non-denatured, specially filtered whey proteins on the market and is designed to help boost your daily protein intake. What’s so great about protein? Well, protein consists of amino acids, which are the building blocks for muscle tissue. Among the criti ...
Symposium Poster - uospur
... The siP peptide binds to both gA6 and mA6, leading us to believe that humans are representative of the orthologs in the s100A6 clade. Binding of the siP peptide is conserved in diverse species. The NCX1 peptide binds to mA5, suggesting that binding of this peptide is conserved in the S100A5 clade. ...
... The siP peptide binds to both gA6 and mA6, leading us to believe that humans are representative of the orthologs in the s100A6 clade. Binding of the siP peptide is conserved in diverse species. The NCX1 peptide binds to mA5, suggesting that binding of this peptide is conserved in the S100A5 clade. ...
Generation of B-cell
... undergo a process called somatic hypermutation. Here an enzyme called activation-induced cytidine deaminase (AID) makes random mutations in the antibody variable region genes. If the mutations result in an antibody that more strongly binds to their targets then these B cells will survive and may dif ...
... undergo a process called somatic hypermutation. Here an enzyme called activation-induced cytidine deaminase (AID) makes random mutations in the antibody variable region genes. If the mutations result in an antibody that more strongly binds to their targets then these B cells will survive and may dif ...
Protein Purification - University of San Diego Home Pages
... Used to separate any of the big four biomolecules Affinity chromatography purification based on a natural interactions for a protein and a substrate or chemical group (ligand) ...
... Used to separate any of the big four biomolecules Affinity chromatography purification based on a natural interactions for a protein and a substrate or chemical group (ligand) ...
Phospho-Src Family (Tyr416) Antibody
... signaling from multiple cell surface receptors such as the B cell antigen receptor (BCR) and CD40 (4). Lck is essential for T-lymphocyte activation and differentiation (5,6). Phosphorylation of the carboxy-terminal Tyr505 downregulates Lck activity, while phosphorylation at Tyr394 leads to an increa ...
... signaling from multiple cell surface receptors such as the B cell antigen receptor (BCR) and CD40 (4). Lck is essential for T-lymphocyte activation and differentiation (5,6). Phosphorylation of the carboxy-terminal Tyr505 downregulates Lck activity, while phosphorylation at Tyr394 leads to an increa ...