* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Cathelicidin Antibody
Survey
Document related concepts
Hygiene hypothesis wikipedia , lookup
Infection control wikipedia , lookup
Anti-nuclear antibody wikipedia , lookup
DNA vaccination wikipedia , lookup
Innate immune system wikipedia , lookup
Polyclonal B cell response wikipedia , lookup
Immunoprecipitation wikipedia , lookup
Gluten immunochemistry wikipedia , lookup
Immunocontraception wikipedia , lookup
Molecular mimicry wikipedia , lookup
Cancer immunotherapy wikipedia , lookup
Immunosuppressive drug wikipedia , lookup
Transcript
Cathelicidin Antibody CATALOG NUMBER: 4429 Western blot analysis of Cathelicidin in Immunofluorescence of Cathelicidin in Immunohistochemistry of Cathelicidin in Human spleen tissue lysate with Human Spleen cells with Cathelicidin human spleen tissue with Cathelicidin Cathelicidin antibody at 1 ug/mL. antibody at 20 ug/mL. antibody at 5 ug/ml. Immunohistochemistry of Catheli-cidin in human spleen tissue with Cathelicidin antibody at 5 ug/mL. Specifications SPECIES REACTIVITY: Human TESTED APPLICATIONS: ELISA, IF, IHC-P, WB APPLICATIONS: Cathelicidin antibody can be used for detection of Cathelicidin by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. USER NOTE: Optimal dilutions for each application to be determined by the researcher. POSITIVE CONTROL: 1) Cat. No. 1306 - Human Spleen Tissue Lysate PREDICTED MOLECULAR Predicted: 19 kDa WEIGHT: Observed: 18 kDa IMMUNOGEN: Cathelicidin antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human Cathelicidin. The immunogen is located within amino acids 50 - 100 of Cathelicidin. HOST SPECIES: Properties Rabbit PURIFICATION: Cathelicidin Antibody is affinity chromatography purified via peptide column. PHYSICAL STATE: Liquid BUFFER: Cathelicidin Antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL STORAGE CONDITIONS: Cathelicidin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated Additional Info ALTERNATE NAMES: Cathelicidin Antibody: LL37, CAP18, CRAMP, HSD26, CAP-18, FALL39, FALL-39, Cathelicidin antimicrobial peptide, 18 kDa cationic antimicrobial protein ACCESSION NO.: NP_004336 PROTEIN GI NO.: 39753970 OFFICIAL SYMBOL: CAMP GENE ID: 820 Background BACKGROUND: Cathelicidin Antibody: One component of host defense at mucosal surfaces is epithelial-derived antimicrobial peptides. Cathelicidins are one family of antimicrobial peptides characterized by conserved pro-peptide sequences that have been identified in epithelial tissues and some myeloid cells of humans and animals. LL37/hCAP-18 is the only Cathelicidin found in humans and is expressed in inflammatory and epithelial cells. The presence of these molecules is essential for defense against invasive bacterial infection in skin. Besides their direct antimicrobial function, Cathelicidins have multiple roles in mediating innate and adaptive immunity, such as endotoxin neutralizing, angiogenesis, wound healing and promoting neutrophil chemotaxis and mast cell recruitment. Finally, Cathelicidin antimicrobial peptides qualify as prototypes of innovative drugs that may be used to treat infection and/or modulate the immune response. REFERENCES: 1) Zaiou M and Gallo RL. Cathelicidins, essential gene-encoded mammalian antibiotics. J. Mol. Med. 2002; 80:549-61. 2) Agerberth B, Gunne H, Odeberg J, et al. FALL-39, a Putative Human Peptide Antibiotic, is Cysteine-Free and Expressed in Bone Marrow and Testis. Proc. Natl. Acad. Sci. USA 1995; 92:195-9. 3) Nizet V, Ohtake T, Lauthe X, et al. Innate antimicrobial peptide protects the skin from invasive bacterial infection. Nature 2001; 414:454-7. 4) Koczulla R, von Degenfeld G, Kupatt C, et al. An angiogenic role for the human peptide antibiotic LL-37/hCAP18. J. Clin. Invest. 2003; 111:1665-72. FOR RESEARCH USE ONLY December 12, 2016