Promoter Analysis of the Mouse Sterol Regulatory Element
... family, SREBPs are synthesized as precursor proteins that remain bound to the endoplasmic reticulum and the nuclear envelope in the presence of sufficient sterol concentrations. Upon sterol deprivation, the precursor protein undergoes a sequential two-step cleavage process to release the NH2-termina ...
... family, SREBPs are synthesized as precursor proteins that remain bound to the endoplasmic reticulum and the nuclear envelope in the presence of sufficient sterol concentrations. Upon sterol deprivation, the precursor protein undergoes a sequential two-step cleavage process to release the NH2-termina ...
Photosynthesis genes in marine viruses yield proteins during host
... photosystem II core reaction centre protein D1, and high-lightinducible (hli) genes. Here we show that phage psbA and hli genes are expressed during infection of Prochlorococcus and are cotranscribed with essential phage capsid genes, and that the amount of phage D1 protein increases steadily over t ...
... photosystem II core reaction centre protein D1, and high-lightinducible (hli) genes. Here we show that phage psbA and hli genes are expressed during infection of Prochlorococcus and are cotranscribed with essential phage capsid genes, and that the amount of phage D1 protein increases steadily over t ...
Chemical Synthesis of Oligonucleotides
... because the non-full-length mass might interfere with the applications. Similarly, oligonucleotides modified with non-standard bases, fluorescent dyes, linkers, etc. should be subjected to additional purifications. IDT uses two methods for post-production purification: 1. Polyacrylamide gel electrop ...
... because the non-full-length mass might interfere with the applications. Similarly, oligonucleotides modified with non-standard bases, fluorescent dyes, linkers, etc. should be subjected to additional purifications. IDT uses two methods for post-production purification: 1. Polyacrylamide gel electrop ...
DNA and Protein Production
... strand comes from taking the other two phosphates off. The energy gained from breaking the bonds is used to build the new bond ...
... strand comes from taking the other two phosphates off. The energy gained from breaking the bonds is used to build the new bond ...
Down-regulation of acetolactate synthase compromises Ol
... allele in the resistant genotype encodes an isoform of the enzyme with altered kinetic properties compared with the isoform in susceptible genotypes. This altered SHMT enzyme is likely associated with perturbation of the folate pathway resulting in nutritional deficiency for nematodes. Overexpressio ...
... allele in the resistant genotype encodes an isoform of the enzyme with altered kinetic properties compared with the isoform in susceptible genotypes. This altered SHMT enzyme is likely associated with perturbation of the folate pathway resulting in nutritional deficiency for nematodes. Overexpressio ...
Is structural flexibility of antigen-binding loops
... of vibration of VH CDR3 among five clones and found that such vibration of BW9-7 was most restrained (Fig. 5). Because amino acid sequences of VH CDR3 of BW9-7 were identical to those of BW9-9, the observed limited vibration of BW9-7 VH CDR3 may be due to interaction with other loops located close t ...
... of vibration of VH CDR3 among five clones and found that such vibration of BW9-7 was most restrained (Fig. 5). Because amino acid sequences of VH CDR3 of BW9-7 were identical to those of BW9-9, the observed limited vibration of BW9-7 VH CDR3 may be due to interaction with other loops located close t ...
Cell Differentiation during Sexual Development of the Fungus
... each primer and 1.25 U of Goldstar polymerase (Eurogentec, Cologne, Germany) in a total volume of 50 ml. The amplification reaction consisted of 40 cycles of 1 min at 92°C, 1 min at 50 to 55°C (depending on the primers used), and 1 to 1.5 min at 72°C (depending on the length of the amplification pro ...
... each primer and 1.25 U of Goldstar polymerase (Eurogentec, Cologne, Germany) in a total volume of 50 ml. The amplification reaction consisted of 40 cycles of 1 min at 92°C, 1 min at 50 to 55°C (depending on the primers used), and 1 to 1.5 min at 72°C (depending on the length of the amplification pro ...
Full-Text PDF
... fully answered. Expression analysis of pollen allergens does not yet have any specific protocols or methods developed, despite a very good sequence background available in public bioinformatics databases. However, research in this area of interest has a great application potential for breeding and b ...
... fully answered. Expression analysis of pollen allergens does not yet have any specific protocols or methods developed, despite a very good sequence background available in public bioinformatics databases. However, research in this area of interest has a great application potential for breeding and b ...
Analysis of gene expression changes in Trichophyton rubrum after
... levels of 15 genes in the study were estimated by quantitative realtime RT-PCR. Aliquots of the RNA preparations from samples at each of the time points used in the microarray experiments were saved for quantitative real-time RT-PCR. Gene-specific primers were designed for the genes and the 18S rRNA ...
... levels of 15 genes in the study were estimated by quantitative realtime RT-PCR. Aliquots of the RNA preparations from samples at each of the time points used in the microarray experiments were saved for quantitative real-time RT-PCR. Gene-specific primers were designed for the genes and the 18S rRNA ...
Identification of a Fluorescent Protein from Rhacostoma Atlantica
... designed based on the amino acid stretch GKLPVPWPT in A. victoria. This highly conserved sequence includes a turn leading into the central helix of the protein. The degenerate primer was used in conjunction with a primer containing a poly T repeat to amplify potential FPs from a cDNA library of a Rh ...
... designed based on the amino acid stretch GKLPVPWPT in A. victoria. This highly conserved sequence includes a turn leading into the central helix of the protein. The degenerate primer was used in conjunction with a primer containing a poly T repeat to amplify potential FPs from a cDNA library of a Rh ...
The DnaE polymerase from Deinococcus radiodurans features
... (αDr). The αDr enzyme was overexpressed in Escherichia coli, both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E. coli RNA polymerase, from which αDr could not be dissociated. On the contrary, when refolded from inc ...
... (αDr). The αDr enzyme was overexpressed in Escherichia coli, both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E. coli RNA polymerase, from which αDr could not be dissociated. On the contrary, when refolded from inc ...
Bioinformatics Molecular Genetics
... and other intervening nucleotide sequences. • In the chromosomes of eukaryotes, the DNA exists in a quasi-ordered structure inside the nucleus, where it wraps around histones (structural proteins), and where this composite material is called chromatin. • Prokaryotes do not possess histones or nuclei ...
... and other intervening nucleotide sequences. • In the chromosomes of eukaryotes, the DNA exists in a quasi-ordered structure inside the nucleus, where it wraps around histones (structural proteins), and where this composite material is called chromatin. • Prokaryotes do not possess histones or nuclei ...
The Role of the C-terminal Tail of the Ribosomal Protein S13 in Pr
... in appendix a (1‐5). The primers have 30 ~ 40 nt homologous to the rpsM gene, followed by the modified S13 CTD sequence, a stop codon, an E. coli SD sequence for translation of amp resistance gene, and short sequence complementary to the amp resistance gene in pND707. ...
... in appendix a (1‐5). The primers have 30 ~ 40 nt homologous to the rpsM gene, followed by the modified S13 CTD sequence, a stop codon, an E. coli SD sequence for translation of amp resistance gene, and short sequence complementary to the amp resistance gene in pND707. ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... Because several nucleotide codons can code for the same amino acid, target DNA sequences that have differing codon usages may require degeneracy in the primers even if the sequence is conserved on the amino acid level. In addition, some amino acid residues may not be conserved in the regions of gene ...
... Because several nucleotide codons can code for the same amino acid, target DNA sequences that have differing codon usages may require degeneracy in the primers even if the sequence is conserved on the amino acid level. In addition, some amino acid residues may not be conserved in the regions of gene ...
From The Building Blocks to Life
... cytosine (pyrimidines) are bound to the ribose. Proteins are polymers of amino acids linked by peptide bonds. In contemporary life there are 20 different amino acids in proteins of general formula NH2-CHR-COOH that differ in the structure of the side chain R group. In one scenario for the origin of ...
... cytosine (pyrimidines) are bound to the ribose. Proteins are polymers of amino acids linked by peptide bonds. In contemporary life there are 20 different amino acids in proteins of general formula NH2-CHR-COOH that differ in the structure of the side chain R group. In one scenario for the origin of ...
Appendix A: General Remarks on Handling RNA
... DNA during RNA purification. The DNase is efficiently removed in subsequent wash steps. Note: Standard DNase buffers are not compatible with on-membrane DNase digestion. Use of other buffers may affect the binding of the RNA to the RNeasy silica-gel membrane, reducing the yield and integrity of the ...
... DNA during RNA purification. The DNase is efficiently removed in subsequent wash steps. Note: Standard DNase buffers are not compatible with on-membrane DNase digestion. Use of other buffers may affect the binding of the RNA to the RNeasy silica-gel membrane, reducing the yield and integrity of the ...
(mmg) operon of Bacillus
... various industries. Hence this organism is extensively studied. The genome of this bacterium has around 4000 protein coding sequences, which include 87% of the genome sequence. Because of its ability to use different carbohydrates, the glycolytic pathway along with the TCA cycle is utilized in this ...
... various industries. Hence this organism is extensively studied. The genome of this bacterium has around 4000 protein coding sequences, which include 87% of the genome sequence. Because of its ability to use different carbohydrates, the glycolytic pathway along with the TCA cycle is utilized in this ...
Catabolic Alanine Racemase from Salmonella typhimurium: DNA Sequence, Enzyme Purification, and Characterization.
... emtimate protein concentration by as much as 1.5-fold. When this corrected concentration was used, the racemase specific activity was calculated to be 1400 units/mg at pH 9.2 with 50 mM L-alanine as substrate. Physical and Kinetic Characterization. Comparison of the denatured (39 000) and native (50 ...
... emtimate protein concentration by as much as 1.5-fold. When this corrected concentration was used, the racemase specific activity was calculated to be 1400 units/mg at pH 9.2 with 50 mM L-alanine as substrate. Physical and Kinetic Characterization. Comparison of the denatured (39 000) and native (50 ...
How to measure chromatin modifications
... profiling of subgenomic regions. ChIP–SAGE is also more expensive than ChIP–Seq because it uses the more expensive traditional sequencing methods. Options ChIP–Seq does not require pre-selection of genomic regions whereas ChIP–chip can only analyse the portion of the genome on a microarray. Although ...
... profiling of subgenomic regions. ChIP–SAGE is also more expensive than ChIP–Seq because it uses the more expensive traditional sequencing methods. Options ChIP–Seq does not require pre-selection of genomic regions whereas ChIP–chip can only analyse the portion of the genome on a microarray. Although ...
DNA recognition code of transcription factors
... molecular biology (DNA makes RNA makes protein) was proposed (Crick, 1958). Despite its remarkable verification, it is being seen increasingly as limited, for if the whole flow of information in a cell were unidirectional, all cells with the same complement of genetic material would have identical f ...
... molecular biology (DNA makes RNA makes protein) was proposed (Crick, 1958). Despite its remarkable verification, it is being seen increasingly as limited, for if the whole flow of information in a cell were unidirectional, all cells with the same complement of genetic material would have identical f ...
2.277 December 2004 Final Exam
... 1. Integral membrane proteins interact with fatty acid “tails” of bilayers 2. Peripheral membrane proteins behave like typical soluble proteins when released from the bilayer. 3. Peripheral membrane proteins are released from the membrane by treatment with ...
... 1. Integral membrane proteins interact with fatty acid “tails” of bilayers 2. Peripheral membrane proteins behave like typical soluble proteins when released from the bilayer. 3. Peripheral membrane proteins are released from the membrane by treatment with ...
International Journal of Antimicrobial Agents Mechanism of drug
... pathogen that causes gastroenteritis that is clinically indistinguishable from cholera [1–4]. Information regarding this human pathogen is limited because the enteritis caused by this organism is not as frequent as that caused by Vibrio cholerae [5]. However, in recent years it is being isolated wit ...
... pathogen that causes gastroenteritis that is clinically indistinguishable from cholera [1–4]. Information regarding this human pathogen is limited because the enteritis caused by this organism is not as frequent as that caused by Vibrio cholerae [5]. However, in recent years it is being isolated wit ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.