Molecular Cloning and Nucleotide Sequence of the 3
... the first report of the nucleotide sequence of a functional gene of C. utilis as far as we know. The sequence of 2209 bp was an alignment of four restriction fragments determined separately. The sequences of HindIII-CluI, CluI-EcoRI, EcoRI-BglII and BglII-Hind111 fragments could be clearly read from ...
... the first report of the nucleotide sequence of a functional gene of C. utilis as far as we know. The sequence of 2209 bp was an alignment of four restriction fragments determined separately. The sequences of HindIII-CluI, CluI-EcoRI, EcoRI-BglII and BglII-Hind111 fragments could be clearly read from ...
Chapter 3 Kinetic analysis of ribozyme cleavage
... migration after renaturation; this could reflect dissociation of a dimer or formation of a more compact tertiary fold (18), both of which are typically desired. Run markers on the gel; double-stranded DNA (dsDNA) markers are readily available from numerous vendors (e.g. New England Biolabs), and doub ...
... migration after renaturation; this could reflect dissociation of a dimer or formation of a more compact tertiary fold (18), both of which are typically desired. Run markers on the gel; double-stranded DNA (dsDNA) markers are readily available from numerous vendors (e.g. New England Biolabs), and doub ...
Worksheet 2
... The lowest position no. of these four numbers is: __________________ The highest position no. of these four numbers is: __________________ These two numbers stretch a region of how many nucleotides? __________________ Record the gi-number for this entry: _______________________________________ What ...
... The lowest position no. of these four numbers is: __________________ The highest position no. of these four numbers is: __________________ These two numbers stretch a region of how many nucleotides? __________________ Record the gi-number for this entry: _______________________________________ What ...
Structure and expression of the PHO80 gene of Saccharomyces
... In yeast, the repression of acid phosphatase under high phosphate growth conditions requires the trans-acting factor PHO80. We have determined the DNA sequence of the PHO80 gene and found that it encodes a protein of 293 amino acids. The expression of the PHO80 gene, as measured by Northern analysis ...
... In yeast, the repression of acid phosphatase under high phosphate growth conditions requires the trans-acting factor PHO80. We have determined the DNA sequence of the PHO80 gene and found that it encodes a protein of 293 amino acids. The expression of the PHO80 gene, as measured by Northern analysis ...
GeneCensus - Gerstein Lab Publications
... terms of the attributes of a single gene (e.g. sequence similarity for a particular ortholog). The comparisons are presented in a visual fashion over the web at GeneCensus.org. The system concentrates on two types of comparisons: (i) trees based on the sharing of generalized protein families between ...
... terms of the attributes of a single gene (e.g. sequence similarity for a particular ortholog). The comparisons are presented in a visual fashion over the web at GeneCensus.org. The system concentrates on two types of comparisons: (i) trees based on the sharing of generalized protein families between ...
The Arabidopsis ABHD11 Mutant Accumulates
... AtABHD11. Both phospholipase and lysophospholipase activities were carried out with purified proteins from recombinant AtABHD11 and empty vector, and the assays were carried out for 30 min at 30˚C. Assay contained 50 mM NBD-fluorescent fatty acid-labeled lyso(phospho)lipids substrates in the presenc ...
... AtABHD11. Both phospholipase and lysophospholipase activities were carried out with purified proteins from recombinant AtABHD11 and empty vector, and the assays were carried out for 30 min at 30˚C. Assay contained 50 mM NBD-fluorescent fatty acid-labeled lyso(phospho)lipids substrates in the presenc ...
Structure of DIG
... Shown together, the DIG label is used, via an antibody via AP, to produce light in the location on the blot where DNA matching your probe resides CSPD ...
... Shown together, the DIG label is used, via an antibody via AP, to produce light in the location on the blot where DNA matching your probe resides CSPD ...
GeneCensus - Gerstein Lab Publications
... terms of the attributes of a single gene (e.g. sequence similarity for a particular ortholog). The comparisons are presented in a visual fashion over the web at GeneCensus.org. The system concentrates on two types of comparisons: (i) trees based on the sharing of generalized protein families between ...
... terms of the attributes of a single gene (e.g. sequence similarity for a particular ortholog). The comparisons are presented in a visual fashion over the web at GeneCensus.org. The system concentrates on two types of comparisons: (i) trees based on the sharing of generalized protein families between ...
Capillary Electrophoresis of Oligonucleotides
... length of an oligonucleotide can be estimated by CE using known size standards. For example, the size standard used by IDT is a purified 40-mer that is used to define a retention time for the capillary. For example, if the 40-mer standard has a retention time of 22 minutes and the average time betwe ...
... length of an oligonucleotide can be estimated by CE using known size standards. For example, the size standard used by IDT is a purified 40-mer that is used to define a retention time for the capillary. For example, if the 40-mer standard has a retention time of 22 minutes and the average time betwe ...
Copy of NAR30_7.book(gkf263.fm)
... moiety, which possesses three dimethoxyltrityl (DMT) protected hydroxyl groups, is used for subsequent thiol functionalization (21). Following removal of the three DMT groups with trichloroacetic acid in methylene chloride, the resulting triol 5 was further coupled with three equivalents of hexyldis ...
... moiety, which possesses three dimethoxyltrityl (DMT) protected hydroxyl groups, is used for subsequent thiol functionalization (21). Following removal of the three DMT groups with trichloroacetic acid in methylene chloride, the resulting triol 5 was further coupled with three equivalents of hexyldis ...
Arabidopsis Gene and cDNA Encoding Cell
... fragment containing Atbfructl was identified by screening a genomic library (EMBL3, Clontech, Palo Alto, CA) with a 1kb fragment from a cDNA encoding a cell-wall invertase in D. carota (Sturm and Chrispeels, 1990). The Atbfructl cDNA clone was identified by screening an A. thaliana cDNA library with ...
... fragment containing Atbfructl was identified by screening a genomic library (EMBL3, Clontech, Palo Alto, CA) with a 1kb fragment from a cDNA encoding a cell-wall invertase in D. carota (Sturm and Chrispeels, 1990). The Atbfructl cDNA clone was identified by screening an A. thaliana cDNA library with ...
IS1245 restriction fragment length polymorphism typing - HAL
... NruI and SmaI are blunt-end cleavers, after ligation both restriction sites are lost. However, the IS1245 insert can be removed by using restriction enzymes SphI and EcoRI. In PCR, 10 to 20 ng of undigested plasmid DNA is sufficient to ensure an optimal DNA target concentration. The accurate determi ...
... NruI and SmaI are blunt-end cleavers, after ligation both restriction sites are lost. However, the IS1245 insert can be removed by using restriction enzymes SphI and EcoRI. In PCR, 10 to 20 ng of undigested plasmid DNA is sufficient to ensure an optimal DNA target concentration. The accurate determi ...
The role of xylulokinase in Saccharomyces cerevisiae xylulose
... the forward reactions were about 600 nkat mg31 (640 with D-xylulose, 500 with ATP). Km and Vmax were obtained from Hanes^Woolf plots of the presented data. All these data were obtained under similar conditions at the same pH (pH 6.5). From these data, an equilibrium constant of 90 was calculated usi ...
... the forward reactions were about 600 nkat mg31 (640 with D-xylulose, 500 with ATP). Km and Vmax were obtained from Hanes^Woolf plots of the presented data. All these data were obtained under similar conditions at the same pH (pH 6.5). From these data, an equilibrium constant of 90 was calculated usi ...
Proposed alignment of helical interruptions in the two subunits of the
... and 8, Ala is substituted for Gly. Either one of these substitutions will not affect the physical parameters of the collagen molecule substantially (Mao, B. and Vogeli, G., unpublished data from computer modelling). It is, however, also possible that the cyz (IV) chain was the ancestral gene with ma ...
... and 8, Ala is substituted for Gly. Either one of these substitutions will not affect the physical parameters of the collagen molecule substantially (Mao, B. and Vogeli, G., unpublished data from computer modelling). It is, however, also possible that the cyz (IV) chain was the ancestral gene with ma ...
Chapter 5: Nucleic Acids, etc. Nucleotides and Derivatives Nucleic
... Nucleic acids are synthesized from 5'-nucleoside triphosphates in a 5' to 3' direction ...
... Nucleic acids are synthesized from 5'-nucleoside triphosphates in a 5' to 3' direction ...
... In the case of DNA, electrostatic repulsion between the phosphates on opposite strands is very unfavorable for formation of double stranded DNA. The positively charged ions will screen these charges from each other, making the DNA more stable as the salt concentration is increased. (+3 pts) In the c ...
in Power-Point Format
... • During initiation s recycled for additional use in process called the s cycle • Core enzyme can release s; associates with another core enzyme • Red [g -32P]ATP; then RifR core + Rif (green) or –Rif (blue) ...
... • During initiation s recycled for additional use in process called the s cycle • Core enzyme can release s; associates with another core enzyme • Red [g -32P]ATP; then RifR core + Rif (green) or –Rif (blue) ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.