Molecular analysis of extracellular-superoxide dismutase
... cDNA 002947 in EMBL database) has been reported by Hjalmarsson et al. (1987). Based on the sequence, 10 specific oligonucleotide primers were designed for polymerase chain reaction (PCR) and direct sequencing (Fig. 1). The entire coding region of EC-SOD was amplified as two separate DNA fragments; P ...
... cDNA 002947 in EMBL database) has been reported by Hjalmarsson et al. (1987). Based on the sequence, 10 specific oligonucleotide primers were designed for polymerase chain reaction (PCR) and direct sequencing (Fig. 1). The entire coding region of EC-SOD was amplified as two separate DNA fragments; P ...
Systems-wide Chicken DNA Microarrays, Gene Expression Profiling
... that is focused on development of DNA microarrays from chicken EST, global gene expression profiling, and functional mapping of growth regulating genes in the broiler chicken. The most promising genetic resources for such goals are two populations of broiler chickens divergently selected for extreme ...
... that is focused on development of DNA microarrays from chicken EST, global gene expression profiling, and functional mapping of growth regulating genes in the broiler chicken. The most promising genetic resources for such goals are two populations of broiler chickens divergently selected for extreme ...
The serC-aroA operon of Escherichia coli
... be readily observed in the 'high-activity' crude extracts. Unfortunately, in wild type E. coli there is an abundant protein which co-migrates with EPSP synthase. This has been identified as elongation factor Tu which has an Mr of 43225 (Arai et al., 1979) and under these conditions it is not separat ...
... be readily observed in the 'high-activity' crude extracts. Unfortunately, in wild type E. coli there is an abundant protein which co-migrates with EPSP synthase. This has been identified as elongation factor Tu which has an Mr of 43225 (Arai et al., 1979) and under these conditions it is not separat ...
spectroscopic studies of mosquito iridescent virus, its capsid
... Optical spectroscopy is one of the efficient and informative methods of studying viruses. It provides well reproducible results and is a powerful supplement to conventional biological methods of studying the living organisms. Thus, it allows one to determine the chemical composition of the capsid pr ...
... Optical spectroscopy is one of the efficient and informative methods of studying viruses. It provides well reproducible results and is a powerful supplement to conventional biological methods of studying the living organisms. Thus, it allows one to determine the chemical composition of the capsid pr ...
Cloning and characterization of a phosphopantetheinyl transferase
... PKS modules [28^32]. BLM is an antitumor drug belonging to a group of natural products of hybrid peptide^polyketide origin, synthesized by a hybrid NRPS^PKS, the Blm NRPS/PKS/NRPS megasynthetase [30]. This prompted us to search for a PPTase from S. verticillus in order to use it either in vitro or i ...
... PKS modules [28^32]. BLM is an antitumor drug belonging to a group of natural products of hybrid peptide^polyketide origin, synthesized by a hybrid NRPS^PKS, the Blm NRPS/PKS/NRPS megasynthetase [30]. This prompted us to search for a PPTase from S. verticillus in order to use it either in vitro or i ...
MB206_fhs_lnt_001.1_AT_May09
... The DNA-directed synthesis of RNA is called transcription. Transcription produces RNA molecules that are complimentary copies of one strand of DNA. Only one of the dsDNA strands can serve as template for synthesis of a specific mRNA molecule. mRNAs transmit information from DNA, and each mRN ...
... The DNA-directed synthesis of RNA is called transcription. Transcription produces RNA molecules that are complimentary copies of one strand of DNA. Only one of the dsDNA strands can serve as template for synthesis of a specific mRNA molecule. mRNAs transmit information from DNA, and each mRN ...
Biology
... 27. (1pt) The “guardian angel gene” codes for a protein that will destroy the cell that it is in, if it turns cancerous. If the mutation in the previous question occurs in p53, how many of the cells resulting from mitosis of this cell will carry the mutation? ...
... 27. (1pt) The “guardian angel gene” codes for a protein that will destroy the cell that it is in, if it turns cancerous. If the mutation in the previous question occurs in p53, how many of the cells resulting from mitosis of this cell will carry the mutation? ...
Differential expression of six genes in fat
... Total RNA was isolated using TRIzol Reagent (Thermo Fisher Scientific, USA) and 1-bromo-3-chloropropane (VWR International, USA). Three isolations were carried out for every experimental animal and for each tissue. Samples (150–200 mg) were processed with TissueLyser LT (Qiagen, Germany) for each is ...
... Total RNA was isolated using TRIzol Reagent (Thermo Fisher Scientific, USA) and 1-bromo-3-chloropropane (VWR International, USA). Three isolations were carried out for every experimental animal and for each tissue. Samples (150–200 mg) were processed with TissueLyser LT (Qiagen, Germany) for each is ...
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis
... the other hand has the lowest buffering capacity but provides the best resolution for larger DNA which implies the need for lower voltage and more time with a better product. Lithium Borate (LB) - relatively new buffer and is ineffective in resolving fragments larger than 5 kbp. However, with its lo ...
... the other hand has the lowest buffering capacity but provides the best resolution for larger DNA which implies the need for lower voltage and more time with a better product. Lithium Borate (LB) - relatively new buffer and is ineffective in resolving fragments larger than 5 kbp. However, with its lo ...
Function of lanI in regulation of landomycin A biosynthesis in
... duction of either lanI or lndI [lanI homologue controlling landomycin E biosynthesis in Streptomyces globisporus 1912, both encoding Streptomyces antibiotic regulatory proteins (SARPs)] restored landomycin A production in the mutant strain. Chimeric constructs ladI and ladR were generated by exchang ...
... duction of either lanI or lndI [lanI homologue controlling landomycin E biosynthesis in Streptomyces globisporus 1912, both encoding Streptomyces antibiotic regulatory proteins (SARPs)] restored landomycin A production in the mutant strain. Chimeric constructs ladI and ladR were generated by exchang ...
Section 3: Prokaryotic Sample and Array Processing
... standard procedures for bacterial RNA isolation or various commercial RNA isolation kits. For Pseudomonas aeruginosa and E. coli, we have successfully used the QIAGEN® RNeasy Mini Purification Kit. Caution should be used to minimize chromosomal DNA contamination during the isolation, due to the high ...
... standard procedures for bacterial RNA isolation or various commercial RNA isolation kits. For Pseudomonas aeruginosa and E. coli, we have successfully used the QIAGEN® RNeasy Mini Purification Kit. Caution should be used to minimize chromosomal DNA contamination during the isolation, due to the high ...
Isolation of the b-tubulin Gene From Yeast and Demonstration of its Essential Function in vivo.
... to use chicken brain 01. and fl-tubulin cDNAs (Cleveland et al., 1980) as hybridization probes for isolating the analogous yeast genes. Gel transfer hybridization experiments (Southern, 1975) were carried out to find hybridization conditions such that the chicken cDNA probes produce a specific and r ...
... to use chicken brain 01. and fl-tubulin cDNAs (Cleveland et al., 1980) as hybridization probes for isolating the analogous yeast genes. Gel transfer hybridization experiments (Southern, 1975) were carried out to find hybridization conditions such that the chicken cDNA probes produce a specific and r ...
Functional second genes generated by retrotransposition of the X
... Although the complete process for assembling the functional ribosome has not yet been elucidated, the in¯uence of the gene dosage of each ribosomal component on development has been extensively studied in Drosophila melanogaster. For example, haploinsuf®ciency of any one of the RP genes yields a Min ...
... Although the complete process for assembling the functional ribosome has not yet been elucidated, the in¯uence of the gene dosage of each ribosomal component on development has been extensively studied in Drosophila melanogaster. For example, haploinsuf®ciency of any one of the RP genes yields a Min ...
Molecular cloning of a rhodopsin gene from salamander rods.
... Ning Chen, Jian-xing Ma,% D. Wesley Corson, E. Starr Hazard, and Rosalie K. Crouch ...
... Ning Chen, Jian-xing Ma,% D. Wesley Corson, E. Starr Hazard, and Rosalie K. Crouch ...
Characterisation of the diol dehydratase pdu operon of Lactobacillus
... The genes of the enzyme diol dehydratase are part of a putative operon of at least seven genes. The highest identities were found with K. oxytoca. However, it was surprising to discover similarities with the 5P extremity of the pdu operon of S. enterica. Two genes located upstream of the genes of th ...
... The genes of the enzyme diol dehydratase are part of a putative operon of at least seven genes. The highest identities were found with K. oxytoca. However, it was surprising to discover similarities with the 5P extremity of the pdu operon of S. enterica. Two genes located upstream of the genes of th ...
Base excision repair
... coding regions can also be silent. However, some noncoding sequences do have essential functions in gene regulation and, in this case, mutations in these sequences would have phenotypic effects. A nucleotide substitution that creates a new stop codon is called a nonsense mutation. It causes prematur ...
... coding regions can also be silent. However, some noncoding sequences do have essential functions in gene regulation and, in this case, mutations in these sequences would have phenotypic effects. A nucleotide substitution that creates a new stop codon is called a nonsense mutation. It causes prematur ...
Protocol S1.
... interaction between a regulator and a target is considered independently. These methods do not explicitly model the functional form of a combinatorial interaction, but they are more computationally tractable and have no problems scaling to genome-sized networks. In this group we tested relevance net ...
... interaction between a regulator and a target is considered independently. These methods do not explicitly model the functional form of a combinatorial interaction, but they are more computationally tractable and have no problems scaling to genome-sized networks. In this group we tested relevance net ...
supplementary methods
... and B54 Dra (AS) primers (Table S1) using BGT54 DNA as template. B54 Dra (AS) primer was used to create a DraI site within intron 2. The PCR product was digested with BamHI and DraI, subcloned into pGEM-T and confirmed by sequencing. Second, the 5' A-globin region of intron 2 was released from pGEM ...
... and B54 Dra (AS) primers (Table S1) using BGT54 DNA as template. B54 Dra (AS) primer was used to create a DraI site within intron 2. The PCR product was digested with BamHI and DraI, subcloned into pGEM-T and confirmed by sequencing. Second, the 5' A-globin region of intron 2 was released from pGEM ...
A-level Human Biology Question Paper Unit 04 - Bodies and
... Wrapping a person in blankets leads to an immediate warming of the skin. This stimulates thermoreceptors in the skin. This stimulation of thermoreceptors in the skin causes the fall in core body temperature seen in the first ten minutes. Suggest how. ...
... Wrapping a person in blankets leads to an immediate warming of the skin. This stimulates thermoreceptors in the skin. This stimulation of thermoreceptors in the skin causes the fall in core body temperature seen in the first ten minutes. Suggest how. ...
Genomic analysis and comparison of very virulent infectious bursal
... cDNA of vvIBDV 3529/92 (from Virus Research Institutes Malaysia) was used as a template for amplification of segment A gene. Full length segment A ORF2 was amplified by separated amplification divided into three fragments. For each fragment, amplifications were conducted by identifying unique native ...
... cDNA of vvIBDV 3529/92 (from Virus Research Institutes Malaysia) was used as a template for amplification of segment A gene. Full length segment A ORF2 was amplified by separated amplification divided into three fragments. For each fragment, amplifications were conducted by identifying unique native ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.