マニュアル Megaprime DNA Labelling System
... 10 minutes incubation at 37°C. This rapid labelling is achieved by the use of nonamer primers rather than the conventional hexamers (Figure 1). Nonamers allow for more efficient priming from the template DNA at 37°C, resulting in fast and efficient labelling of the DNA. A new alternative protocol ha ...
... 10 minutes incubation at 37°C. This rapid labelling is achieved by the use of nonamer primers rather than the conventional hexamers (Figure 1). Nonamers allow for more efficient priming from the template DNA at 37°C, resulting in fast and efficient labelling of the DNA. A new alternative protocol ha ...
Folate and DNA methylation during in utero development and aging
... of the vertebrate genome occurs with age in most tissues (reviewed in [8]) accompanied by aberrant hypermethylation in the promoter regions of genes, e.g. ER, IGF2 and cmyc (reviewed in [8]). Such age-related methylation has been implicated as an early step in carcinogenesis [9]. Therefore, not only ...
... of the vertebrate genome occurs with age in most tissues (reviewed in [8]) accompanied by aberrant hypermethylation in the promoter regions of genes, e.g. ER, IGF2 and cmyc (reviewed in [8]). Such age-related methylation has been implicated as an early step in carcinogenesis [9]. Therefore, not only ...
RNA EXTRACTION
... What is RNA? • RNA = Ribonucleic acid. • A type of nucleic acid with only one strand - ribose instead of deoxyribose and using uracil instead of thymine (in DNA). • Provides the link between the genetic information through protein synthesis (serve as template for protein synthesis). • Total RNA= rR ...
... What is RNA? • RNA = Ribonucleic acid. • A type of nucleic acid with only one strand - ribose instead of deoxyribose and using uracil instead of thymine (in DNA). • Provides the link between the genetic information through protein synthesis (serve as template for protein synthesis). • Total RNA= rR ...
Genbiotech Store
... one minigel. Note: including 0.1 M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the gel stain is reused. 1.3 Place the gel in a suitable container such as a polypropylene staining tray. Add a sufficient amount of the 3X staining solution to submerge the g ...
... one minigel. Note: including 0.1 M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the gel stain is reused. 1.3 Place the gel in a suitable container such as a polypropylene staining tray. Add a sufficient amount of the 3X staining solution to submerge the g ...
A Fruit-Specific Putative Dihydroflavonol 4
... was the DFR from V. vinifera, the fruit of which, like that of strawberry, is considered nonclimacteric. DFR is encoded either by a small multigene family (Beld et al., 1989; Helariutta et al., 1993) or by only one gene (Kristiansen and Rohde, 1991; Bongue-Bartelsman et al., 1994; Sparvoli et al., 1 ...
... was the DFR from V. vinifera, the fruit of which, like that of strawberry, is considered nonclimacteric. DFR is encoded either by a small multigene family (Beld et al., 1989; Helariutta et al., 1993) or by only one gene (Kristiansen and Rohde, 1991; Bongue-Bartelsman et al., 1994; Sparvoli et al., 1 ...
Q1. Lysozyme is an enzyme consisting of a single polypeptide chain
... In an investigation, a culture of Chlamydomonas was treated in a way that caused them to lose their flagella without any other damage to the cells. The flagella grew back to their original length in 60 minutes. How many amino acid molecules would be incorporated into each growing flagellum ...
... In an investigation, a culture of Chlamydomonas was treated in a way that caused them to lose their flagella without any other damage to the cells. The flagella grew back to their original length in 60 minutes. How many amino acid molecules would be incorporated into each growing flagellum ...
Document
... the Brassicaceae SI system, extensive sequence divergence and rearrangements were observed when comparing the S locus of two different S haplotypes (Boyes et al., 1997), suggesting that recombination is likely to be suppressed at the Brassicaceae S locus as well. No recombination has been detected b ...
... the Brassicaceae SI system, extensive sequence divergence and rearrangements were observed when comparing the S locus of two different S haplotypes (Boyes et al., 1997), suggesting that recombination is likely to be suppressed at the Brassicaceae S locus as well. No recombination has been detected b ...
Applied Environmnetal Microbiology
... Bacillus thuringiensis is a gram-positive bacterium that produces proteinaceous crystals during sporulation. Many different B. thuringiensis crystal protein genes have been identified, and the toxicity of the encoded proteins has been investigated. At least four major classes of insecticidal protein ...
... Bacillus thuringiensis is a gram-positive bacterium that produces proteinaceous crystals during sporulation. Many different B. thuringiensis crystal protein genes have been identified, and the toxicity of the encoded proteins has been investigated. At least four major classes of insecticidal protein ...
Retroposed New Genes Out of the X in Drosophila
... from the X chromosome, it is unclear whether or not this observation is limited to the identified new genes in the group defined by 70% amino acid identity. Thus, we extended a similar analysis (see Methods) to the new retrogenes of 50% or higher identity at the amino acid level with their parental ...
... from the X chromosome, it is unclear whether or not this observation is limited to the identified new genes in the group defined by 70% amino acid identity. Thus, we extended a similar analysis (see Methods) to the new retrogenes of 50% or higher identity at the amino acid level with their parental ...
Transcription is the synthesis of RNA under the direction of DNA
... Although RNA polymerase traverses the template strand from 3' → 5', the coding (nontemplate) strand is usually used as the reference point, so transcription is said to go from 5' → 3'. This produces an RNA molecule from 5' → 3', an exact copy of the coding strand (except that thymines are replaced w ...
... Although RNA polymerase traverses the template strand from 3' → 5', the coding (nontemplate) strand is usually used as the reference point, so transcription is said to go from 5' → 3'. This produces an RNA molecule from 5' → 3', an exact copy of the coding strand (except that thymines are replaced w ...
Cloning and nucleotide sequence of a gene upstream of the eaeA
... revealed no reaction with mAb 4E8C12 despite the fact that protein samples from clone 6-F were prepared using several different methods. Southern blot analysis was performed to determine if clone 6-F contained the same gene interrupted by Tn phoA in the OMP- mutant strain, A 10. The insert DNA from ...
... revealed no reaction with mAb 4E8C12 despite the fact that protein samples from clone 6-F were prepared using several different methods. Southern blot analysis was performed to determine if clone 6-F contained the same gene interrupted by Tn phoA in the OMP- mutant strain, A 10. The insert DNA from ...
Full Text - Genome Biology and Evolution
... initially prepared from isolated RNA (10 mg) through the use of GenElute mRNA Miniprep Kit (Sigma-Aldrich, Tokyo, Japan), which was reverse transcribed to cDNA with ReverTra Ace in a 20 ml reaction volume. In both cases, the cDNA reaction mixture was diluted 1:10 using DNase, RNase-free molecular bi ...
... initially prepared from isolated RNA (10 mg) through the use of GenElute mRNA Miniprep Kit (Sigma-Aldrich, Tokyo, Japan), which was reverse transcribed to cDNA with ReverTra Ace in a 20 ml reaction volume. In both cases, the cDNA reaction mixture was diluted 1:10 using DNase, RNase-free molecular bi ...
Next generation sequencing
... SMRT is carried out with incorporation of nucleotides that give off a base specific fluorescent signal on each addition. The templates are distributed one per well (or more exactly, there is one DNA polymerase per well). The wells are constructed with highly sensitive sensors so that the signal from ...
... SMRT is carried out with incorporation of nucleotides that give off a base specific fluorescent signal on each addition. The templates are distributed one per well (or more exactly, there is one DNA polymerase per well). The wells are constructed with highly sensitive sensors so that the signal from ...
Detection of Antioxidative Activity of Plant Extracts at the DNA-modified Screen-printed Electrode
... sensor in solution of a high ionic strength (100 mM phosphate buffer pH 7.0) under stirring during 120 s. A negligible marker peak current was checked by the DPV record in blank. DNA damage and effect of antioxidants were detected by the measurement of the DPV marker signal (as described above) afte ...
... sensor in solution of a high ionic strength (100 mM phosphate buffer pH 7.0) under stirring during 120 s. A negligible marker peak current was checked by the DPV record in blank. DNA damage and effect of antioxidants were detected by the measurement of the DPV marker signal (as described above) afte ...
ScrFl restriction/modification system from
... shown to specifically recognize 5’ CCNGG 3’ sites, cleaving after the second cytosine and the degenerate central base. The ENase gene (scrFIR; 862 bp) was located between, and co-directionally transcribed with, two formerly characterized 5-methylcytosine methyltransferase genes, which encode protein ...
... shown to specifically recognize 5’ CCNGG 3’ sites, cleaving after the second cytosine and the degenerate central base. The ENase gene (scrFIR; 862 bp) was located between, and co-directionally transcribed with, two formerly characterized 5-methylcytosine methyltransferase genes, which encode protein ...
297: High-Throughput Molecular Diagnostics for Rapid Detection of
... • Pathogen vs. Contaminant? • Sampling Bias? • Poor Detection by Ibis T5000 Biosensor? • What is the “Gold Standard”? • Molecular Diagnostics Excluding CNS ...
... • Pathogen vs. Contaminant? • Sampling Bias? • Poor Detection by Ibis T5000 Biosensor? • What is the “Gold Standard”? • Molecular Diagnostics Excluding CNS ...
Chapter 12 Lecture Notes: Metabolism – Enzyme and Gene
... A. Why regulate? 1. Bacteria are extremely efficient organisms. It is wasteful to have all 1000 – 2000 metabolic pathways on at the same time. 2. During exponential growth all cellular components are synthesized at constant rates relative to one another (balanced growth). 3. Thus, the cell integrate ...
... A. Why regulate? 1. Bacteria are extremely efficient organisms. It is wasteful to have all 1000 – 2000 metabolic pathways on at the same time. 2. During exponential growth all cellular components are synthesized at constant rates relative to one another (balanced growth). 3. Thus, the cell integrate ...
Cloning and Molecular Analysis of the Plasma ... Paramecium tetraurelia
... Final PCR amplification and sequencing of the plasma membrane Ca2+-ATPase.The entire open reading frame was amplified to produce the full-length plasma membrane Ca2+ pump PCR product for confirming sequencing. Primers were designed from the region 80 bases upstream of the start codon and the region ...
... Final PCR amplification and sequencing of the plasma membrane Ca2+-ATPase.The entire open reading frame was amplified to produce the full-length plasma membrane Ca2+ pump PCR product for confirming sequencing. Primers were designed from the region 80 bases upstream of the start codon and the region ...
Background for the Recombinant DNA Lab
... it contains TAQ polymerase, dNTPs, and buffer. In this case, the components have been dried into a bead, so you’ll dissolve the bead into solution using your primers and DNA. ...
... it contains TAQ polymerase, dNTPs, and buffer. In this case, the components have been dried into a bead, so you’ll dissolve the bead into solution using your primers and DNA. ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.