Lecture 15: Translation and Transcription
... Nucleotide triplets are known as codons (ii) Basic unit of the genetic code (iii) Specify either individual amino acids or termination ...
... Nucleotide triplets are known as codons (ii) Basic unit of the genetic code (iii) Specify either individual amino acids or termination ...
Directions for Use Ribonuclease A (RNase A), 10 mg/mL
... General Information Ribonuclease A (RNase A), 10 mg/mL Solution is prepared from pancreatic RNase A of bovine origin. RNase A is an endoribonuclease that efficiently hydrolyzes RNA contaminants in DNA preparations by cleaving the phosphodiester bond between the 3’-phosphate group of a pyrimidine nuc ...
... General Information Ribonuclease A (RNase A), 10 mg/mL Solution is prepared from pancreatic RNase A of bovine origin. RNase A is an endoribonuclease that efficiently hydrolyzes RNA contaminants in DNA preparations by cleaving the phosphodiester bond between the 3’-phosphate group of a pyrimidine nuc ...
Articles (Danaher) ) , short, fluorescently
... limited to ~109 bases per run1. Finally, the inherent sensitivity of fluorescence allows fluorescence-based sequencing methods to use >103 times fewer DNA templates than pyrodetection methods1,3, reducing reagent volumes and costs4–8. However, the multiple chemical steps required in each sequencin ...
... limited to ~109 bases per run1. Finally, the inherent sensitivity of fluorescence allows fluorescence-based sequencing methods to use >103 times fewer DNA templates than pyrodetection methods1,3, reducing reagent volumes and costs4–8. However, the multiple chemical steps required in each sequencin ...
Text Book of Molecular Biology
... The three dimensional structure of DNA Ⅰ.DNA double-helix structure is the secondary structure of DNA DNA double-helix structure model was put forth in 1953 by Watson and Crick. DNA double-helix model 1 Two separate and anti-parallel chains of DNA are wound around each other in a right-handed helica ...
... The three dimensional structure of DNA Ⅰ.DNA double-helix structure is the secondary structure of DNA DNA double-helix structure model was put forth in 1953 by Watson and Crick. DNA double-helix model 1 Two separate and anti-parallel chains of DNA are wound around each other in a right-handed helica ...
Interaction of DNA with ribosomes in cell-free protein
... are found. Electron micrographs of such polysomes show a direct association of the DNA molecule with several ribosomes. 5. In Chlorella, direct translation of DNA can be obtained also in the presence of neomycin. The kinetics of this reaction are different from those of endogenous m-RNA mediated and ...
... are found. Electron micrographs of such polysomes show a direct association of the DNA molecule with several ribosomes. 5. In Chlorella, direct translation of DNA can be obtained also in the presence of neomycin. The kinetics of this reaction are different from those of endogenous m-RNA mediated and ...
Lactobacilli carry cryptic genes encoding peptidase
... For cloning of pepQ, the upstream and coding regions of the pepQ gene were cloned in pJDC9 as a PCR product synthesized with the primer 5' TTGTGCAATCGCTTACAG 3' (p290), designed according to the upstream region of Lb. delbrueckii subsp. l a d s pepQ (Stucky et al., 1995) and with the Lb. delbrueckii ...
... For cloning of pepQ, the upstream and coding regions of the pepQ gene were cloned in pJDC9 as a PCR product synthesized with the primer 5' TTGTGCAATCGCTTACAG 3' (p290), designed according to the upstream region of Lb. delbrueckii subsp. l a d s pepQ (Stucky et al., 1995) and with the Lb. delbrueckii ...
document
... timing. Thanks to its single-photon sensitivity and time-resolved measurement capability, faint fluorescence signals were successfully detected from Cy5 labeled DNA molecules. The molecules were placed directly on the surface of the imager. The new approach for on-chip fluorescence detection reporte ...
... timing. Thanks to its single-photon sensitivity and time-resolved measurement capability, faint fluorescence signals were successfully detected from Cy5 labeled DNA molecules. The molecules were placed directly on the surface of the imager. The new approach for on-chip fluorescence detection reporte ...
Lecture 9
... •After washing away all of the unstuck RNA, the microarray can be observed under a microscope and it can be determined which RNA remains stuck to the DNA spots •Microarray technology can be used to learn which genes are expressed differently in a target sample compared to a control sample (e.g disea ...
... •After washing away all of the unstuck RNA, the microarray can be observed under a microscope and it can be determined which RNA remains stuck to the DNA spots •Microarray technology can be used to learn which genes are expressed differently in a target sample compared to a control sample (e.g disea ...
Normalization between a pair of arrays
... •After washing away all of the unstuck RNA, the microarray can be observed under a microscope and it can be determined which RNA remains stuck to the DNA spots •Microarray technology can be used to learn which genes are expressed differently in a target sample compared to a control sample (e.g disea ...
... •After washing away all of the unstuck RNA, the microarray can be observed under a microscope and it can be determined which RNA remains stuck to the DNA spots •Microarray technology can be used to learn which genes are expressed differently in a target sample compared to a control sample (e.g disea ...
Molecular Evolution of Functional Nucleic Acids
... These results indicate that the zinc ion, with which imidazoyl group is coordinated, could effectively work as catalyst. Perrin et al. obtained a modified DNA enzyme from a library of doubly modified DNA involving both a deoxyuridine analog with allylamine at the 5 position and a deoxyadenosine anal ...
... These results indicate that the zinc ion, with which imidazoyl group is coordinated, could effectively work as catalyst. Perrin et al. obtained a modified DNA enzyme from a library of doubly modified DNA involving both a deoxyuridine analog with allylamine at the 5 position and a deoxyadenosine anal ...
methodology for high-quality RNA extraction from poultry whole
... to develop a protocol for total RNA extraction using avian whole blood by defining the effect of anticoagulants and sample purification protocols on RNA yield and quality. 2. Blood collections from the cutaneous ulnar or medial metatarsal veins of birds yielded adequate blood volume (2–3 ml) draws. Th ...
... to develop a protocol for total RNA extraction using avian whole blood by defining the effect of anticoagulants and sample purification protocols on RNA yield and quality. 2. Blood collections from the cutaneous ulnar or medial metatarsal veins of birds yielded adequate blood volume (2–3 ml) draws. Th ...
PDF ( 33 ) - DergiPark
... s for 30 cycles, followed by 72 °C for 5 min. To detect the PCR products, 1% agarose gel electrophoresis was used. The results were scanned and analyzed using the Gel-Pro Analyzer 4 program. Table. Reaction primer of PCR. Gene name GPRC5D ...
... s for 30 cycles, followed by 72 °C for 5 min. To detect the PCR products, 1% agarose gel electrophoresis was used. The results were scanned and analyzed using the Gel-Pro Analyzer 4 program. Table. Reaction primer of PCR. Gene name GPRC5D ...
Complete Elimination of Endosymbiotic Algae from Paramecium
... single species or not. We have previously cloned endosymbiotic algae from P. bursaria and characterization of each clone was carried out (Nishihara et al. 1998). On the other hand, we have reported a new technique to produce algae-free paramecia by treating green paramecia with a herbicide, paraquat ...
... single species or not. We have previously cloned endosymbiotic algae from P. bursaria and characterization of each clone was carried out (Nishihara et al. 1998). On the other hand, we have reported a new technique to produce algae-free paramecia by treating green paramecia with a herbicide, paraquat ...
Isolation, cloning and sequence analysis of the lactate
... years without the development of any resistance to these drugs. However, resistance to buparvaquone has been recently reported for the first time in the literature (Mhadhbi et al. 2010) and this has very recently been followed by a new case (Sharifiyazdi et al. 2012). The first study was conducted i ...
... years without the development of any resistance to these drugs. However, resistance to buparvaquone has been recently reported for the first time in the literature (Mhadhbi et al. 2010) and this has very recently been followed by a new case (Sharifiyazdi et al. 2012). The first study was conducted i ...
PDF
... Results presented in this paper show that the unstable repficon, pSA3, can be used as d vector to integrate D N A into the L. plantarum genome. The major advantage of this system, compared to the use of suicide vectors [3] is that the latter requires a high transformation frequency. In the approach ...
... Results presented in this paper show that the unstable repficon, pSA3, can be used as d vector to integrate D N A into the L. plantarum genome. The major advantage of this system, compared to the use of suicide vectors [3] is that the latter requires a high transformation frequency. In the approach ...
A tale of two functions: enzymatic activity and
... Detection of CT–RNA interaction in situ Detection of CT binding to RNA in situ was accomplished using a fluorescence resonance energy transfer (FRET) assay with a CT b-enhanced yellow fluorescent protein (eYFP) fusion protein as the donor and the RNA binding dye Sytox Orange (Molecular Probes) as the ...
... Detection of CT–RNA interaction in situ Detection of CT binding to RNA in situ was accomplished using a fluorescence resonance energy transfer (FRET) assay with a CT b-enhanced yellow fluorescent protein (eYFP) fusion protein as the donor and the RNA binding dye Sytox Orange (Molecular Probes) as the ...
Expression of a mouse replacement histone H3. 3 gene with a
... latter correspond to the histone replacement variant mRNAs encoded by the pmH3.3 cDNA. Northern blot analysis using subclone C comprising the 3' end of the cDNA (positions +1163 to +1544) as probe, revealed only the 1.8 kb mRNA. This mRNA therefore most probably results from the use of the polyadeny ...
... latter correspond to the histone replacement variant mRNAs encoded by the pmH3.3 cDNA. Northern blot analysis using subclone C comprising the 3' end of the cDNA (positions +1163 to +1544) as probe, revealed only the 1.8 kb mRNA. This mRNA therefore most probably results from the use of the polyadeny ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.