Psittacine Beak and Feather Disease Virus Nucleotide Sequence
... features of the genome were most closely related to PCV. Like PCV (Meehan et al., 1997), BFDV contained seven major ORFs and lacked a distinctive noncoding region, thus affording highly efficient use of genetic material in both of these viruses. Both viruses have three ORFs in the encapsidated stran ...
... features of the genome were most closely related to PCV. Like PCV (Meehan et al., 1997), BFDV contained seven major ORFs and lacked a distinctive noncoding region, thus affording highly efficient use of genetic material in both of these viruses. Both viruses have three ORFs in the encapsidated stran ...
Life Without Water: Expression of Plant LEA Genes - The Keep
... mixes were prepared by using the IQ SYBR Green Supermix (Bio-Rad Laboratories) with a total reaction volume of 25 ml. Cycling parameters were 3 min at 951C and then 50 cycles at 951C (15 sec) and 561C (30 sec), followed by a melting curve analysis. Each developmental stage was evaluated with 4–6 ind ...
... mixes were prepared by using the IQ SYBR Green Supermix (Bio-Rad Laboratories) with a total reaction volume of 25 ml. Cycling parameters were 3 min at 951C and then 50 cycles at 951C (15 sec) and 561C (30 sec), followed by a melting curve analysis. Each developmental stage was evaluated with 4–6 ind ...
Identification of sixteen single-nucleotide polymorphism markers in
... Primers were first examined for PCR in eight wild individuals. PCR was performed in 20 µL reaction volume containing 12.3 µL H2 O miliQ, 2 µL PCR buffer (Mg2+ Plus) 10×, 1.6 µL dNTP 2.5 mM, 0.8 µL of each primer 10 µM, 0.2 µL Taq DNA polymerase (TaKaRa, Dalian, China) 5 U/µL and 1.5 µL of genomic DN ...
... Primers were first examined for PCR in eight wild individuals. PCR was performed in 20 µL reaction volume containing 12.3 µL H2 O miliQ, 2 µL PCR buffer (Mg2+ Plus) 10×, 1.6 µL dNTP 2.5 mM, 0.8 µL of each primer 10 µM, 0.2 µL Taq DNA polymerase (TaKaRa, Dalian, China) 5 U/µL and 1.5 µL of genomic DN ...
Comparative day/night metatranscriptomic analysis of microbial
... technically difficult, however, as prokaryotic mRNAs generally lack the poly(A) tails that make isolation of eukaryotic messages relatively straightforward (Liang and Pardee, 1992) and because of the relatively short halflives of mRNAs (Belasco, 1993). In addition, mRNAs are much less abundant than ...
... technically difficult, however, as prokaryotic mRNAs generally lack the poly(A) tails that make isolation of eukaryotic messages relatively straightforward (Liang and Pardee, 1992) and because of the relatively short halflives of mRNAs (Belasco, 1993). In addition, mRNAs are much less abundant than ...
Nucleotide excision repair II: from yeast to mammals
... is the most prominent characteristic of trichothiodystrophy, a disorder that also affectspatientswho are not photosensitive (reviewed in Ref. 6). NER deficiency is reflected at the cellular level as hypersensitivity to UV and to agents that mimic the effect of UV; in most XP and PIBIDS patients, UVi ...
... is the most prominent characteristic of trichothiodystrophy, a disorder that also affectspatientswho are not photosensitive (reviewed in Ref. 6). NER deficiency is reflected at the cellular level as hypersensitivity to UV and to agents that mimic the effect of UV; in most XP and PIBIDS patients, UVi ...
HY asiakirjapohja - Hercules Project
... sequencing of some pilot samples. The existing sequence data have been produced with Illumina HiSeq4000 (RNAseq, methylome sequencing, exome sequencing), HiSeqX10 (WGS) and Illumina MiSeq/NexSeq500 (targeted/ amplicon sequencing). We have also developed a bioinformatics infrastructure (Anduril, http ...
... sequencing of some pilot samples. The existing sequence data have been produced with Illumina HiSeq4000 (RNAseq, methylome sequencing, exome sequencing), HiSeqX10 (WGS) and Illumina MiSeq/NexSeq500 (targeted/ amplicon sequencing). We have also developed a bioinformatics infrastructure (Anduril, http ...
Part 1
... • The total info stored in all chromosomes constitutes a genome • In most multi-cell organisms, every cell contains the same complete set of chromosomes – May have some small different due to mutation ...
... • The total info stored in all chromosomes constitutes a genome • In most multi-cell organisms, every cell contains the same complete set of chromosomes – May have some small different due to mutation ...
The DNA sequence of the gene and genetic control sites for the
... subtil is RNA polymerase. This is followed by a sequence resemb1 ing a B.subtilis ribosome binding site nine nucleotides before the first codon of the gene. Two sequences, one before and one after the gene, can be arranged in secondary structures similar to transcriptional terminators. There is also ...
... subtil is RNA polymerase. This is followed by a sequence resemb1 ing a B.subtilis ribosome binding site nine nucleotides before the first codon of the gene. Two sequences, one before and one after the gene, can be arranged in secondary structures similar to transcriptional terminators. There is also ...
Reassembled Biosynthetic Pathway for Large
... achieves the same goal with the use of only one plasmid and a single strain. All the enzymes essential for oligosaccharide synthesis, including the glycosyltransferases, and the sugar ± nucleotide regeneration are in one E. coli strain. Thus, this approach avoids unnecessary transport of the interme ...
... achieves the same goal with the use of only one plasmid and a single strain. All the enzymes essential for oligosaccharide synthesis, including the glycosyltransferases, and the sugar ± nucleotide regeneration are in one E. coli strain. Thus, this approach avoids unnecessary transport of the interme ...
Journal of Biotechnology VI-2 Genomics, proteomics and
... H. elongata IFO15536 had a very low yield of total ectoine released, 2.89 g/L, than that of strain EG6 under same conditions (Table 1). In addition, growth of strain EG6 was highly increased during downshock treatment in comparison to H. elongata IFO15536. Thus, we concluded that 0.3 M NaCl was the ...
... H. elongata IFO15536 had a very low yield of total ectoine released, 2.89 g/L, than that of strain EG6 under same conditions (Table 1). In addition, growth of strain EG6 was highly increased during downshock treatment in comparison to H. elongata IFO15536. Thus, we concluded that 0.3 M NaCl was the ...
The Euglena gracilis chloroplast rpoB gene
... We are interested in the relationship between chloroplast genes for RNA polymerase subunits and the known chloroplast polymerase activities. Antibodies against fusion proteins that contained fragments of the chloroplast genes rpoA from spinach, rpoB from tobacco, and rpoC2 from Euglena, were able to ...
... We are interested in the relationship between chloroplast genes for RNA polymerase subunits and the known chloroplast polymerase activities. Antibodies against fusion proteins that contained fragments of the chloroplast genes rpoA from spinach, rpoB from tobacco, and rpoC2 from Euglena, were able to ...
Transcriptome Atlas
... been fundamental to study gene expression in many plants; however, this technology is biased toward known RNAs used to generate the probes in the chips. With the advent of next-generation sequencing (RNA-Seq), global RNA analysis (transcriptome) is becoming routine for many plant species. RNA-Se ...
... been fundamental to study gene expression in many plants; however, this technology is biased toward known RNAs used to generate the probes in the chips. With the advent of next-generation sequencing (RNA-Seq), global RNA analysis (transcriptome) is becoming routine for many plant species. RNA-Se ...
... elongation during synthesis of proteins on the ribosome. Your answer should include a brief description of the molecules involved, both proteins and nucleic acids, as well as a clear indication of the order of events during the process. A well labeled sketch would be an acceptable answer (10 pts). ...
RNA Isolation and Technology Applications
... Following hybridization, the RNA is digested with RNases specific for single-stranded nucleic acids – Any remaining unhybridized single-stranded RNA target and ...
... Following hybridization, the RNA is digested with RNases specific for single-stranded nucleic acids – Any remaining unhybridized single-stranded RNA target and ...
Applied and Environmental Microbiology
... 72°C for 10 min. Amplified PCR products were analyzed by gel electrophoresis with 0.8% agarose in TAE buffer (40 mM Tris-acetate [pH 7.6], 1 mM Na2EDTA). A DIG-labeled double-stranded DNA probe for NPRC was generated by PCR with the DIG DNA labeling kit (Boehringer) and the primers npr BcI and npr B ...
... 72°C for 10 min. Amplified PCR products were analyzed by gel electrophoresis with 0.8% agarose in TAE buffer (40 mM Tris-acetate [pH 7.6], 1 mM Na2EDTA). A DIG-labeled double-stranded DNA probe for NPRC was generated by PCR with the DIG DNA labeling kit (Boehringer) and the primers npr BcI and npr B ...
JSB-302
... The JSB-302 is designed for nucleic acid screening electrophoresis. The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the JSB-302 is 150 volts. In a 1% TBE gel, this translates into a run time of approximately 1 hour. Lower voltages may be used, of course, an ...
... The JSB-302 is designed for nucleic acid screening electrophoresis. The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the JSB-302 is 150 volts. In a 1% TBE gel, this translates into a run time of approximately 1 hour. Lower voltages may be used, of course, an ...
FREE Sample Here
... rapidly replaced the 15N media with 14N, or light nitrogen. DNA was extracted at various time intervals during the growth of the bacteria, representing different stages of replication (generations). They examined the DNA using density-gradient equilibrium sedimentation and observed that no “heavy” D ...
... rapidly replaced the 15N media with 14N, or light nitrogen. DNA was extracted at various time intervals during the growth of the bacteria, representing different stages of replication (generations). They examined the DNA using density-gradient equilibrium sedimentation and observed that no “heavy” D ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.