Protein-nucleic acid interactions
... Zipper-type proteins — The leucine zipper (bZIP) contains an a-helix with a leucine at every 7th amino acid. The leucines act as the teeth of a zipper that allows dimerization of two proteins. Basic amino acids bind to the sugar-phosphate backbone while the helices fit in the major grooves on opposi ...
... Zipper-type proteins — The leucine zipper (bZIP) contains an a-helix with a leucine at every 7th amino acid. The leucines act as the teeth of a zipper that allows dimerization of two proteins. Basic amino acids bind to the sugar-phosphate backbone while the helices fit in the major grooves on opposi ...
Guide to preparation of liquid biopsies for nucleic acid extraction
... batch, operator or time point are characterized and variability minimized. In addition, samples should be collected under similar conditions and where possible at the same time, relative to e.g. treatment or food intake. If elimination of these sources of variation is not feasible, ensure these “noi ...
... batch, operator or time point are characterized and variability minimized. In addition, samples should be collected under similar conditions and where possible at the same time, relative to e.g. treatment or food intake. If elimination of these sources of variation is not feasible, ensure these “noi ...
C photosynthesis in terrestrial plants does not require Kranz anatomy
... efficiency. Thus, novel traits that improve water-use efficiency, such as single-cell C4 photosynthesis, might have had a competitor-free space in which to evolve. In recent years, there has been much interest in turning C3 crops, such as rice, into C4 plants [15,16]. The major barriers to this goal ...
... efficiency. Thus, novel traits that improve water-use efficiency, such as single-cell C4 photosynthesis, might have had a competitor-free space in which to evolve. In recent years, there has been much interest in turning C3 crops, such as rice, into C4 plants [15,16]. The major barriers to this goal ...
Cloning and characterization of CmGPD1, the Candida magnoliae
... For expression of the CmGPD1 gene in E. coli, a primer pair, N- and C-termini-specific GPD5 (5 0 -GGACTAGTATGAGT TACGCTAAGAAGTTCAAG-3 0 , SpeI site is underlined) and GPD6 (5 0 -CCCAAGCTTCTATTTACAAGCTATCCTCGA GC-3 0 , HindIII site is underlined) was designed based on the full-length genomic CmGPD1 s ...
... For expression of the CmGPD1 gene in E. coli, a primer pair, N- and C-termini-specific GPD5 (5 0 -GGACTAGTATGAGT TACGCTAAGAAGTTCAAG-3 0 , SpeI site is underlined) and GPD6 (5 0 -CCCAAGCTTCTATTTACAAGCTATCCTCGA GC-3 0 , HindIII site is underlined) was designed based on the full-length genomic CmGPD1 s ...
A Drosophila Third Chromosome Minute Locus Encodes
... M i n u t e s are non-additive in their phenotypic effect, i. e., the phenotype of a M I / + ; M 2 / + fly is not more extreme than the phenotypeof any ofthe single mutants. He concluded that the genes code for proteins with similar function (s) . The non-additive property of this type of mutations ...
... M i n u t e s are non-additive in their phenotypic effect, i. e., the phenotype of a M I / + ; M 2 / + fly is not more extreme than the phenotypeof any ofthe single mutants. He concluded that the genes code for proteins with similar function (s) . The non-additive property of this type of mutations ...
The evolutionary history of the stearoyl
... the obese phenotype when on a high fat diet [10]. There are also some naturally occurring SCD1 deficient mice strains (asebia mouse), which, amongst other traits have impaired triglyceride biosynthesis [11]. In fishes, the impact of dietary lipid contents and temperature has also been reported [12]. ...
... the obese phenotype when on a high fat diet [10]. There are also some naturally occurring SCD1 deficient mice strains (asebia mouse), which, amongst other traits have impaired triglyceride biosynthesis [11]. In fishes, the impact of dietary lipid contents and temperature has also been reported [12]. ...
Sequence, Transcription and Translation of a Late Gene of the
... of the p34.8 gene and fusion with the fl-galactosidase gene suggest that the former gene is essential for viral replication. INTRODUCTION Autographa californica nuclear polyhedrosis virus (AcMNPV) serves as a model system for molecular biological studies of baculoviruses (Doerfler & B6hm, 1986) whic ...
... of the p34.8 gene and fusion with the fl-galactosidase gene suggest that the former gene is essential for viral replication. INTRODUCTION Autographa californica nuclear polyhedrosis virus (AcMNPV) serves as a model system for molecular biological studies of baculoviruses (Doerfler & B6hm, 1986) whic ...
記錄 編號 3862 狀態 NC090FJU00112010 助教 查核 索書 號 學校
... gene region. Appl. Environ. Microbiol. 65: 2184-2194. Reimmann, C., and Haas, D. 1987. Mode of replicon fusion mediated by the duplicated insertion sequence IS21 in Escherichia coli. Genetics 115: 619-625. Reimmann, C., and Haas, D. 1990. The istA gene of insertion sequence IS21 is essential for cle ...
... gene region. Appl. Environ. Microbiol. 65: 2184-2194. Reimmann, C., and Haas, D. 1987. Mode of replicon fusion mediated by the duplicated insertion sequence IS21 in Escherichia coli. Genetics 115: 619-625. Reimmann, C., and Haas, D. 1990. The istA gene of insertion sequence IS21 is essential for cle ...
03g - Protein Synth other roles of DNA
... Once its amino acid is released, tRNA is ratcheted to the E site and then released to reenter the cytoplasmic pool, ready to be recharged with a new amino acid. ...
... Once its amino acid is released, tRNA is ratcheted to the E site and then released to reenter the cytoplasmic pool, ready to be recharged with a new amino acid. ...
GelRed™ Product Information Sheet
... one minigel. Note: including 0.1 M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the gel stain is reused. 1.3 Place the gel in a suitable container such as a polypropylene staining tray. Add a sufficient amount of the 3X staining solution to submerge the g ...
... one minigel. Note: including 0.1 M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the gel stain is reused. 1.3 Place the gel in a suitable container such as a polypropylene staining tray. Add a sufficient amount of the 3X staining solution to submerge the g ...
Unzipping Genes - HiMedia Laboratories
... Safety Data Sheet (MSDS) for information regarding hazards and safe handling practices. Introduction RDP TrioTM Reagent is a quick and convenient reagent to use in the isolation of RNA, DNA and Proteins from human (tissue), animal and plant samples. The protocol is rapid and permits isolation from l ...
... Safety Data Sheet (MSDS) for information regarding hazards and safe handling practices. Introduction RDP TrioTM Reagent is a quick and convenient reagent to use in the isolation of RNA, DNA and Proteins from human (tissue), animal and plant samples. The protocol is rapid and permits isolation from l ...
Parathyroid
... The deduced amino acid sequences of PTH gene between 12 species of nonhuman primates and human revealed no amino acid substitution in mature PTH among orangutans, chimpanzees, and humans. The results indicated that the PTH gene is very conserved among primates, especially between great apes and huma ...
... The deduced amino acid sequences of PTH gene between 12 species of nonhuman primates and human revealed no amino acid substitution in mature PTH among orangutans, chimpanzees, and humans. The results indicated that the PTH gene is very conserved among primates, especially between great apes and huma ...
Molecular Beacon Product Sheet
... binding of single stranded oligos to specific targets based on structural conformation. Aptamers are single-stranded RNA or DNA oligonucleotides 15 to 60 base in length that bind with high affinity to specific molecular targets; most aptamers to proteins bind with Kds (equilibrium constant) in the r ...
... binding of single stranded oligos to specific targets based on structural conformation. Aptamers are single-stranded RNA or DNA oligonucleotides 15 to 60 base in length that bind with high affinity to specific molecular targets; most aptamers to proteins bind with Kds (equilibrium constant) in the r ...
Carotene genes from cassava-pchavarriaga.pdf
... The tissue-specific carotene accumulation could be a result of upstream promoter regulation ...
... The tissue-specific carotene accumulation could be a result of upstream promoter regulation ...
1 BIOINFORMATICS Bioinformatics, based on National Institutes of
... „WARNING: Left primer is unacceptable: Tm too high”: that means the difference is too big between the melting temperatures (Tm) of the forward and the possible reverse (right) primers. The Tm of the primers determines the annealing temperature where primers bind to the single stranded template DNA. ...
... „WARNING: Left primer is unacceptable: Tm too high”: that means the difference is too big between the melting temperatures (Tm) of the forward and the possible reverse (right) primers. The Tm of the primers determines the annealing temperature where primers bind to the single stranded template DNA. ...
RNA-Seq Sample Recommendations (Craig Praul, PSU and Caitlyn
... Tissue and Cell Isolation Investigators need to carefully choose their methods of tissue and cell isolation as the choice of these methods have a direct impact on the quality and quantity of RNA subsequently obtained. If possible total RNA purification should follow tissue/cell dissection immediatel ...
... Tissue and Cell Isolation Investigators need to carefully choose their methods of tissue and cell isolation as the choice of these methods have a direct impact on the quality and quantity of RNA subsequently obtained. If possible total RNA purification should follow tissue/cell dissection immediatel ...
Activity: Invasion of the Snorks
... 1. Create the data charts in your lab book. Make sure to leave enough room to have all of the necessary information present. 2. Using the mRNA from the Snork, find the missing strand of DNA belonging to the Snork. From what we know about the Snorks, the base pairing rules are the same as us. 3. Code ...
... 1. Create the data charts in your lab book. Make sure to leave enough room to have all of the necessary information present. 2. Using the mRNA from the Snork, find the missing strand of DNA belonging to the Snork. From what we know about the Snorks, the base pairing rules are the same as us. 3. Code ...
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... 20.3 Promoter elements are defined by mutations and footprinting 20.4 RNA polymerase I has a bipartite promoter 20.5 RNA polymerase III uses both downstream and upstream promoters 20.6 The startpoint for RNA polymerase II 20.7 TBP is a universal factor 20.8 TBP binds DNA in an unusual way 20.9 The b ...
... 20.3 Promoter elements are defined by mutations and footprinting 20.4 RNA polymerase I has a bipartite promoter 20.5 RNA polymerase III uses both downstream and upstream promoters 20.6 The startpoint for RNA polymerase II 20.7 TBP is a universal factor 20.8 TBP binds DNA in an unusual way 20.9 The b ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.