Transcription | Principles of Biology from Nature Education
... "downstream." Unlike DNA polymerase, RNA polymerase does not need a primer to start transcription. The stretch of DNA that is transcribed into RNA is known as the transcription unit. Transcription has three distinct phases: initiation, elongation and termination. During initiation, with the help of ...
... "downstream." Unlike DNA polymerase, RNA polymerase does not need a primer to start transcription. The stretch of DNA that is transcribed into RNA is known as the transcription unit. Transcription has three distinct phases: initiation, elongation and termination. During initiation, with the help of ...
Lesson Plan Template
... material to life. Students are able to research information that interests them, visualize the concepts, and organize and present their thoughts. The gene mutation song that Robin Shaulis recorded will also be very helpful for learning the topic. Songs are much easier to memorize than a string of fa ...
... material to life. Students are able to research information that interests them, visualize the concepts, and organize and present their thoughts. The gene mutation song that Robin Shaulis recorded will also be very helpful for learning the topic. Songs are much easier to memorize than a string of fa ...
CLONING A LYSINE-RICH PROTEIN GENE FROM POTATO
... proteins in crops such as rice and corn, to improve protein quality by increasing the lysine content. However, there has been little report on cloning genes for lysine-rich proteins. In this article, we present the results of cloning the STtLR gene encoding a lysine-rich protein from Thuong Tin pota ...
... proteins in crops such as rice and corn, to improve protein quality by increasing the lysine content. However, there has been little report on cloning genes for lysine-rich proteins. In this article, we present the results of cloning the STtLR gene encoding a lysine-rich protein from Thuong Tin pota ...
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... Intergeneric conjugation between S. avermitilis and E. coli were performed as described previously by Hopwood et al (Hopwood et al. 1985). Putative exconjugants were regenerated on MS medium containing 50 μg mL-1 apramycin, 25 μg mL-1 nalidixic acid for 5 days to confirm resistance. To eliminate aut ...
... Intergeneric conjugation between S. avermitilis and E. coli were performed as described previously by Hopwood et al (Hopwood et al. 1985). Putative exconjugants were regenerated on MS medium containing 50 μg mL-1 apramycin, 25 μg mL-1 nalidixic acid for 5 days to confirm resistance. To eliminate aut ...
Single-Molecule Fluorescence Using Nucleotide Analogs: A Proof
... and PyC fluorescence at the single-molecule level. Our data show that ssDNA, dsDNA, or RNA containing both 2AP and PyC can be monitored using single-molecule fluorescence and a click chemistry immobilization method. We demonstrate that this approach can be used to monitor DNA and RNA in real time. Thi ...
... and PyC fluorescence at the single-molecule level. Our data show that ssDNA, dsDNA, or RNA containing both 2AP and PyC can be monitored using single-molecule fluorescence and a click chemistry immobilization method. We demonstrate that this approach can be used to monitor DNA and RNA in real time. Thi ...
... Although both catalyze the same reaction, they are regulated differently. Based on your knowledge of PFK in the liver, and your knowledge of liver and muscle function, suggest how PFK in the muscle might be regulated by both hormonal as well as energy sensing. The simplest example is the response to ...
Monomeric Cyanine Nucleic Acid Stains
... The fluorescence spectra of the monomeric cyanine nucleic acid stains cover the entire visible wavelength range, as shown in Figure 1 and summarized in Table 2. The table also lists optical filter sets and laser line sources suitable for excitation and detection of these dyes. These dyes may also be ...
... The fluorescence spectra of the monomeric cyanine nucleic acid stains cover the entire visible wavelength range, as shown in Figure 1 and summarized in Table 2. The table also lists optical filter sets and laser line sources suitable for excitation and detection of these dyes. These dyes may also be ...
Ex vivo analysis of splicing assays
... dominant negative form that is highly cytotoxic and that is at the origin of the progeria phenotype [Figure 1]. Splicing reporters reproducing splicing regulation that is responsible for the progeria phenotype will be presented in this chapter in order to provide the reader an example of ex vivo spl ...
... dominant negative form that is highly cytotoxic and that is at the origin of the progeria phenotype [Figure 1]. Splicing reporters reproducing splicing regulation that is responsible for the progeria phenotype will be presented in this chapter in order to provide the reader an example of ex vivo spl ...
... Choice B: The addition of NaCl to a protein solution causes little change in the TM of the protein, but the same concentration of salt can have a large effect on the TM of double stranded DNA. Why? Your answer should state the direction of the change of TM for the DNA when salt is added (e.g. higher ...
Week 12_DNA - Saint Joseph High School
... How nucleotides can be linked to form a DNA strand. S designates the sugar component, which is joined with phosphate groups (P) to form the backbone of DNA. Projecting from the backbone are four bases: A, adenine; G, guanine; T, ...
... How nucleotides can be linked to form a DNA strand. S designates the sugar component, which is joined with phosphate groups (P) to form the backbone of DNA. Projecting from the backbone are four bases: A, adenine; G, guanine; T, ...
0 1R L Press Limited, Oxford, England.
... gene, sak, was stably expressed in E. coli and the active product with a molecular weight and an antigenicity indistinguishable from those of authentic staphylokinase was efficiently secreted into the periplasmic space. Thus it can be a useful tool to investigate the mechanism of protein secretion i ...
... gene, sak, was stably expressed in E. coli and the active product with a molecular weight and an antigenicity indistinguishable from those of authentic staphylokinase was efficiently secreted into the periplasmic space. Thus it can be a useful tool to investigate the mechanism of protein secretion i ...
The cloning and expression characterization of the centrosome
... genes were cloned from testis cDNA library[13,14]. The results suggest that centrin-1 may play specific roles during spermatogenesis. Then we studied the expression characterization of centrin-1 at different developmental stages of spermatogenic cell by RT-PCR. The results showed that centrin-1 mRNA ...
... genes were cloned from testis cDNA library[13,14]. The results suggest that centrin-1 may play specific roles during spermatogenesis. Then we studied the expression characterization of centrin-1 at different developmental stages of spermatogenic cell by RT-PCR. The results showed that centrin-1 mRNA ...
Two fatty acid ∆9-desaturase genes, ole1 and ole2
... Genes encoding two distinct fatty acid ∆9-desaturases were isolated from strains of the oleaginous fungus Mortierella alpina. Two genomic sequences, ∆9-1 and ∆9-2, each containing a single intron, were cloned from strain CBS 528.72 while one cDNA clone, LM9, was isolated from strain CBS 210.32. The ...
... Genes encoding two distinct fatty acid ∆9-desaturases were isolated from strains of the oleaginous fungus Mortierella alpina. Two genomic sequences, ∆9-1 and ∆9-2, each containing a single intron, were cloned from strain CBS 528.72 while one cDNA clone, LM9, was isolated from strain CBS 210.32. The ...
Cloning and characterization in Escherichia coli of the gene
... protein with apparent molecular mass of 71 kDa was found on SDS-PAGE (Fig. 3). We puri¢ed the protein of apparent molecular mass of 71 kDa as described in Section 2. During puri¢cation, however, the amount of the protein of 71 kDa gradually decreased and di¡erent species with apparent molecular mass ...
... protein with apparent molecular mass of 71 kDa was found on SDS-PAGE (Fig. 3). We puri¢ed the protein of apparent molecular mass of 71 kDa as described in Section 2. During puri¢cation, however, the amount of the protein of 71 kDa gradually decreased and di¡erent species with apparent molecular mass ...
1. ATP powers cellular processes by coupling exergonic and
... 65. Water is considered a universal solvent because A) it is an excellent circulating medium B) relatively stable C) it can dissolve ionic compounds, polar covalent molecules and small nonpolar covalent molecules D) contains both positive and negative charged sides E) all of the above 66. The need f ...
... 65. Water is considered a universal solvent because A) it is an excellent circulating medium B) relatively stable C) it can dissolve ionic compounds, polar covalent molecules and small nonpolar covalent molecules D) contains both positive and negative charged sides E) all of the above 66. The need f ...
Product Information FluoroVueTM Nucleic Acid Gel Stain (10,000X
... epi-illuminator or trans-illuminator before/after each use with deionized water. Otherwise, fluorescent dye will accumulate on the surface and cause a high fluorescent background. Video cameras and CCD cameras have a different spectral responses compared to the black-and-white print film and thus ...
... epi-illuminator or trans-illuminator before/after each use with deionized water. Otherwise, fluorescent dye will accumulate on the surface and cause a high fluorescent background. Video cameras and CCD cameras have a different spectral responses compared to the black-and-white print film and thus ...
of translation Initiation: brings together mRNA, a tRNA (with the first
... ribosome carries a specific amino acid at one end and has a specific nucleotide triplet, an anticodon, at the other end. The anticodon base-pairs with a complementary codon on mRNA. – If the codon on mRNA is UUU, a tRNA with an AAA anticodon and carrying phenyalanine will bind to it. Codon by codon, ...
... ribosome carries a specific amino acid at one end and has a specific nucleotide triplet, an anticodon, at the other end. The anticodon base-pairs with a complementary codon on mRNA. – If the codon on mRNA is UUU, a tRNA with an AAA anticodon and carrying phenyalanine will bind to it. Codon by codon, ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.