Characterization of cDNAs Induced in Meiotic Prophase in Lily
... Fig. 3. Comparison of the amino acid sequences of the putative LIM gene products with those of known gene products. The entire or the partial sequences of the putative products of the LIM genes are aligned with those of known gene products. The positions showing amino acid identity are indicated by ...
... Fig. 3. Comparison of the amino acid sequences of the putative LIM gene products with those of known gene products. The entire or the partial sequences of the putative products of the LIM genes are aligned with those of known gene products. The positions showing amino acid identity are indicated by ...
DNA and Genetics 1. Which of the following correctly organizes
... 4. The process of DNA replication begins with one double-stranded molecule of DNA. The two strands of this molecule separate during replication, and DNA polymerases add complementary nucleotides to each strand. The end results of DNA replication are two identical DNA molecules. The process of DNA re ...
... 4. The process of DNA replication begins with one double-stranded molecule of DNA. The two strands of this molecule separate during replication, and DNA polymerases add complementary nucleotides to each strand. The end results of DNA replication are two identical DNA molecules. The process of DNA re ...
Chromatin: a multi-scale jigsaw puzzle
... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
DNA and RNA
... DNA is a blueprint. It does not physically construct anything. Before discussing how the information in the DNA results in the manufacture of a concrete molecule, it is it important to obtain an overall perspective on the genetic code. It is convenient to view the genome for any species as a book wi ...
... DNA is a blueprint. It does not physically construct anything. Before discussing how the information in the DNA results in the manufacture of a concrete molecule, it is it important to obtain an overall perspective on the genetic code. It is convenient to view the genome for any species as a book wi ...
Cloning and characterization of the Xenopus laevis p8 gene
... The Xp8 clone reported here was isolated by a modified PCR screening method. This method has several advantages over standard filter hybridization methods. Screening time is significantly reduced: it took only 48 h to isolate one positive plaque by this method. The method is also technically simple, ...
... The Xp8 clone reported here was isolated by a modified PCR screening method. This method has several advantages over standard filter hybridization methods. Screening time is significantly reduced: it took only 48 h to isolate one positive plaque by this method. The method is also technically simple, ...
Supplemental Material
... differentiation. The choice of the marker is based on a previous observation that the fulllength -gal still retains its activity while fused at the C-terminus of the R protein (WANG et al. 2003). For the current study, only the first 100 amino acid sequence (the fragment) of the -gal was fused ...
... differentiation. The choice of the marker is based on a previous observation that the fulllength -gal still retains its activity while fused at the C-terminus of the R protein (WANG et al. 2003). For the current study, only the first 100 amino acid sequence (the fragment) of the -gal was fused ...
Exam #3 Review Exam #3 will cover from glycolysis to complex
... for phosphorylation of ADP to form ATP! ATP synthase - allows protons pumped out during production of the PMF to pass back into the cell ---> uses energy to fuel the phosphorylation of ADP to produce ATP. This is oxidative phosphorylation! • Practice: If 5 molecules of NADH are completely oxidized b ...
... for phosphorylation of ADP to form ATP! ATP synthase - allows protons pumped out during production of the PMF to pass back into the cell ---> uses energy to fuel the phosphorylation of ADP to produce ATP. This is oxidative phosphorylation! • Practice: If 5 molecules of NADH are completely oxidized b ...
From DNA to Protein: Gene Expression
... the β-globin gene with previously isolated βglobin mRNA as the probe. Viewing the hybridized molecules by electron microscopy, they saw that the introns formed loops—stretches of DNA that did not have complementary base sequences on the ...
... the β-globin gene with previously isolated βglobin mRNA as the probe. Viewing the hybridized molecules by electron microscopy, they saw that the introns formed loops—stretches of DNA that did not have complementary base sequences on the ...
The bond in the bacteriophage 4x174 gene A protein
... results indicate that the reaction of the gene A and A* proteins with these oligonucleotides involves the Iysis of the phosphodiester bond between position 7 (G) and 8 (A), creating a 3 ‘-OH at one end and a tyrosyl-5 ’ -phosphate ester bond at the other end. This type of phosphodiester bond cleavag ...
... results indicate that the reaction of the gene A and A* proteins with these oligonucleotides involves the Iysis of the phosphodiester bond between position 7 (G) and 8 (A), creating a 3 ‘-OH at one end and a tyrosyl-5 ’ -phosphate ester bond at the other end. This type of phosphodiester bond cleavag ...
Rice 5 S Ribosomal RNA and Its Binding Protein Genes: Structure
... (rDNA). The clone contains 10 repeat units of 290 bp, plus 2.0 kb of flanking genomic sequence at one border. Sequencing of individual repeat units shows that the sequence of the 5 S rRNA coding region is very similar to that reported for other flowering plants, while the nontranscrihed spacer regio ...
... (rDNA). The clone contains 10 repeat units of 290 bp, plus 2.0 kb of flanking genomic sequence at one border. Sequencing of individual repeat units shows that the sequence of the 5 S rRNA coding region is very similar to that reported for other flowering plants, while the nontranscrihed spacer regio ...
Assessing Methods of Detecting Osteogenesis Imperfecta.
... RT-PCR 8.5µL of RNA solution was pipetted into a 1.5mL tube and placed onto a 70°C heating block for 5 minutes, then immediately placed in ice. After 5 minutes, while keeping the tube in ice as much as possible, 11.5µL of RT Master Mix was added. The tube was centrifuged briefly to mix the solution ...
... RT-PCR 8.5µL of RNA solution was pipetted into a 1.5mL tube and placed onto a 70°C heating block for 5 minutes, then immediately placed in ice. After 5 minutes, while keeping the tube in ice as much as possible, 11.5µL of RT Master Mix was added. The tube was centrifuged briefly to mix the solution ...
Using Genes for Antibiotic Resistance to Trace Sources of Bacterial
... understood. After these two steps, a recommendation can be made to try and solve the problem. The first part of this experiment will be carried out using a substance called a polymerase chain reaction (PCR), which amplifies the resistance gene. Then agarose gel electrophoresis will be used, so that ...
... understood. After these two steps, a recommendation can be made to try and solve the problem. The first part of this experiment will be carried out using a substance called a polymerase chain reaction (PCR), which amplifies the resistance gene. Then agarose gel electrophoresis will be used, so that ...
Supplementary Information (docx 4776K)
... Kit (TIANGEN) according to the manufacturer’s instructions. The 16S rDNA fragments were amplified by PCR with the primer set, 27F and 1492R (Weisburg et al., 1991). The PCR amplification protocol was as follows: one cycle at 95°C for 3 min, and 35 cycles of 94°C for 1 min, 55°C for 1 min, and 72°C f ...
... Kit (TIANGEN) according to the manufacturer’s instructions. The 16S rDNA fragments were amplified by PCR with the primer set, 27F and 1492R (Weisburg et al., 1991). The PCR amplification protocol was as follows: one cycle at 95°C for 3 min, and 35 cycles of 94°C for 1 min, 55°C for 1 min, and 72°C f ...
EML4-ALK pre-mRNA and mature mRNA fusion detection using
... Gene fusions that activate otherwise silent signaling enzymes, such as the anaplastic lymphoma receptor tyrosine kinase (ALK), are responsible for a significant number of lung and other cancers. Several drugs that target ALK have found success in the treatment of patients with ALK-gene fusions. DN ...
... Gene fusions that activate otherwise silent signaling enzymes, such as the anaplastic lymphoma receptor tyrosine kinase (ALK), are responsible for a significant number of lung and other cancers. Several drugs that target ALK have found success in the treatment of patients with ALK-gene fusions. DN ...
Nucleic Acids Research
... Immunoglobulin G (IgG) molecules in humans are divided into four subclasses based on the presence of particular gamma heavy chain constant regions (Cy). These Cy regions (Cy1 C.Y2, CY3, and Cy4) are encoded by distinct germline genes (1) which are presumed to be the products of gene duplication of a ...
... Immunoglobulin G (IgG) molecules in humans are divided into four subclasses based on the presence of particular gamma heavy chain constant regions (Cy). These Cy regions (Cy1 C.Y2, CY3, and Cy4) are encoded by distinct germline genes (1) which are presumed to be the products of gene duplication of a ...
Info
... Figure I.5. (A) The alphabetic (Greek) labeling of carbons in an alkanoic acid, showing an amino group at the Cα. (B) A Fischer projection showing the Lconfiguration of the naturally occurring amino acids, converted to the standard 3D projection on a 2D surface. The name "amino acid" describes the c ...
... Figure I.5. (A) The alphabetic (Greek) labeling of carbons in an alkanoic acid, showing an amino group at the Cα. (B) A Fischer projection showing the Lconfiguration of the naturally occurring amino acids, converted to the standard 3D projection on a 2D surface. The name "amino acid" describes the c ...
Decoding the Flu
... “Great! You found the start for the HA gene coding region. Here are HA genes data the team had collected for the flu strains that they were studying. We only have information on the start of the gene, but it might be enough.” The virus that the team has contracted probably had a mutation that resul ...
... “Great! You found the start for the HA gene coding region. Here are HA genes data the team had collected for the flu strains that they were studying. We only have information on the start of the gene, but it might be enough.” The virus that the team has contracted probably had a mutation that resul ...
Antisense RNA
... • Formivirsen is the first antisense antiviral drug developed to treat CMV, licensed by FDA in 1998. • Successful reducing the viral load of HIV by developing modified T-Cell, which is antisense to the HIV envelope protein. ...
... • Formivirsen is the first antisense antiviral drug developed to treat CMV, licensed by FDA in 1998. • Successful reducing the viral load of HIV by developing modified T-Cell, which is antisense to the HIV envelope protein. ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.