as a PDF
... were used to amplify the common B, O1, and A102 alleles respectively. Polymerase chain reaction (PCR) amplification was carried out in a reaction volume of 10 µl containing 1 x PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, and 1.5 mM MgCl2), primer mix, 80 µM of each dNTP, 1U Taq polymerase, and 10 ...
... were used to amplify the common B, O1, and A102 alleles respectively. Polymerase chain reaction (PCR) amplification was carried out in a reaction volume of 10 µl containing 1 x PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, and 1.5 mM MgCl2), primer mix, 80 µM of each dNTP, 1U Taq polymerase, and 10 ...
Phylogenetic, amino acid content and indel analyses
... patterns of relationships that depend on the method of analysis used (Olsen et al., 1994 ; Ludwig et al., 1994) and which are sometimes in conflict with those defined either by other gene products, e.g. EF-Tu (Ludwig et al., 1994), ATPase β (Ludwig et al., 1994) and RecA (Eisen, 1995), or by whole g ...
... patterns of relationships that depend on the method of analysis used (Olsen et al., 1994 ; Ludwig et al., 1994) and which are sometimes in conflict with those defined either by other gene products, e.g. EF-Tu (Ludwig et al., 1994), ATPase β (Ludwig et al., 1994) and RecA (Eisen, 1995), or by whole g ...
Isolation and Characterization of a Cytochrome P450 Gene from
... from the conservative region of the F3’5’H genes, a full-length cDNA (accession number AB127340) was cloned from blue flower tepals of Muscari armeniacum ‘Blue Pearl’, and its genomic clone designated MaP450 (accession number AB127341), was isolated from leaves. Nucleotide sequence analysis revealed ...
... from the conservative region of the F3’5’H genes, a full-length cDNA (accession number AB127340) was cloned from blue flower tepals of Muscari armeniacum ‘Blue Pearl’, and its genomic clone designated MaP450 (accession number AB127341), was isolated from leaves. Nucleotide sequence analysis revealed ...
Introduction of Microarray - genomics-lab
... finding and refining biological pathways Mutation and polymorphism detection ...
... finding and refining biological pathways Mutation and polymorphism detection ...
NABP1, a novel RORγ-regulated gene encoding a single
... were generated by alternative splicing. The generation of variant transcripts was confirmed by reverse transcriptase-PCR analysis using DNA-free RNA from thymus and the following forward (F) and reverse (R) primers: 5 -CCTTTCTTCCAGGAATGTGTG-3 (F1), 5 -TCCTTGTGACTTCTCAAAATGC-3 (R1), 5 -AATGGGGAC ...
... were generated by alternative splicing. The generation of variant transcripts was confirmed by reverse transcriptase-PCR analysis using DNA-free RNA from thymus and the following forward (F) and reverse (R) primers: 5 -CCTTTCTTCCAGGAATGTGTG-3 (F1), 5 -TCCTTGTGACTTCTCAAAATGC-3 (R1), 5 -AATGGGGAC ...
recombinant DNA - interactive eBook
... •GM animals can produce pharmaceuticals •GM mice are bred for human disease research •Xenotransplantation (animals as organs donor in ...
... •GM animals can produce pharmaceuticals •GM mice are bred for human disease research •Xenotransplantation (animals as organs donor in ...
Improvement of DNA Extraction Protocols for Nostochopsis spp.
... Nostochopsis spp. are a type of cyanobacteria which form mucilaginous balls. These cyanobacteria have a high content of polysaccharides, which makes it difficult to isolate their genomic DNA by the conventional method. In this research study, six protocols for improvement of DNA extraction from this ...
... Nostochopsis spp. are a type of cyanobacteria which form mucilaginous balls. These cyanobacteria have a high content of polysaccharides, which makes it difficult to isolate their genomic DNA by the conventional method. In this research study, six protocols for improvement of DNA extraction from this ...
Regulation of GFP Expression
... Scientists have engineered plasmids to contain various restriction enzyme sites, so that DNA cut with the same restriction enzyme as the plasmid can be cloned into that plasmid to form recombinant DNA molecules. This is the basis for cloning human or other genes of interest into a carrier, or vector ...
... Scientists have engineered plasmids to contain various restriction enzyme sites, so that DNA cut with the same restriction enzyme as the plasmid can be cloned into that plasmid to form recombinant DNA molecules. This is the basis for cloning human or other genes of interest into a carrier, or vector ...
Two-Dimensional DNA Gel Electrophoresis Mapping: a Novel
... by a 10 min centrifugation at 2540×g. The purified DNA was confirmed by 2% agarose gel electrophoresis and stored in −20°C freezer for subsequent analysis. The yield of DNA extracted from 1 g of each soil sample was determined using the formula DNA yield (µg/g) = 500ABSDNA, where ABSDNA is the DNA a ...
... by a 10 min centrifugation at 2540×g. The purified DNA was confirmed by 2% agarose gel electrophoresis and stored in −20°C freezer for subsequent analysis. The yield of DNA extracted from 1 g of each soil sample was determined using the formula DNA yield (µg/g) = 500ABSDNA, where ABSDNA is the DNA a ...
CAFE: an R package for the detection of gross chromosomal
... The starting point of a CAFE analysis is a set of gene expression microarrays from samples whose e-karyotype will be computed and another (larger) set of microarrays representing controls. The controls define a normal e-karyotype against which the altered e-karyotype will be defined. We recommend ch ...
... The starting point of a CAFE analysis is a set of gene expression microarrays from samples whose e-karyotype will be computed and another (larger) set of microarrays representing controls. The controls define a normal e-karyotype against which the altered e-karyotype will be defined. We recommend ch ...
Roseobacter gallaeciensis sp. nov., a new marine - HAL
... to prepare genomic DNA for PCR. Bacteria grown in MB were centrifuged at 3000 g at 4 °C for 10 min. The pellet obtained was resuspended in 200 µl lysis mixture (10 mM Tris/HCl, pH 8 -0, 1 mM EDTA, 1% Triton X-100) and boiled for 5 min. After a single chloroform extraction, 5 µl supernatant was used ...
... to prepare genomic DNA for PCR. Bacteria grown in MB were centrifuged at 3000 g at 4 °C for 10 min. The pellet obtained was resuspended in 200 µl lysis mixture (10 mM Tris/HCl, pH 8 -0, 1 mM EDTA, 1% Triton X-100) and boiled for 5 min. After a single chloroform extraction, 5 µl supernatant was used ...
PowerPoint 簡報
... •Initiation: A promoter is the DNA sequence that initially binds the RNA polymerase. Only one of the DNA strands acts as a template. The choice of promoter determines which stretch of DNA is transcribed and is the main step at which regulation is imposed. •Elongation: Once the RNA polymerase has sy ...
... •Initiation: A promoter is the DNA sequence that initially binds the RNA polymerase. Only one of the DNA strands acts as a template. The choice of promoter determines which stretch of DNA is transcribed and is the main step at which regulation is imposed. •Elongation: Once the RNA polymerase has sy ...
MS Word - VCU Secrets of the Sequence
... DNA separate and each acts as a template for the synthesis (or replication) of a new strand. New bases are paired with the template strand, and are then connected to one another to form a new strand of DNA. DNA regulates cellular function by directing the creation of certain proteins. It acts as a m ...
... DNA separate and each acts as a template for the synthesis (or replication) of a new strand. New bases are paired with the template strand, and are then connected to one another to form a new strand of DNA. DNA regulates cellular function by directing the creation of certain proteins. It acts as a m ...
Spatial and temporal expression pattern of a novel gene in the frog
... However, a search of the genome of the fish Fugu rubripes, which also lacks a middle ear, identified an a-tectorin homologous to the human a-tectorin. In addition to sequence similarity, this homology assessment was based on the conserved domain structure of the a-tectorins from SMART analysis and t ...
... However, a search of the genome of the fish Fugu rubripes, which also lacks a middle ear, identified an a-tectorin homologous to the human a-tectorin. In addition to sequence similarity, this homology assessment was based on the conserved domain structure of the a-tectorins from SMART analysis and t ...
No Slide Title
... • PPAR activators in primary hepatocytes and adipocytes – Determine fatty acid profiles and metabolic indices – Gene expression profiling • Dietary trial with salmon and sea bream – Measure growth, gene expression, fatty acid profiles ...
... • PPAR activators in primary hepatocytes and adipocytes – Determine fatty acid profiles and metabolic indices – Gene expression profiling • Dietary trial with salmon and sea bream – Measure growth, gene expression, fatty acid profiles ...
Characterization of Complementary DNA Encoding the Precursor for
... involved in proteolytic processing and maturation of GnRH, and a 54-residue associated peptide. Eight of 23 residues in the signal sequence are shared between H. burtoni and at least one of the mammalian forms, not including an analogously placed arginine in the fish and corresponding lysine in all ...
... involved in proteolytic processing and maturation of GnRH, and a 54-residue associated peptide. Eight of 23 residues in the signal sequence are shared between H. burtoni and at least one of the mammalian forms, not including an analogously placed arginine in the fish and corresponding lysine in all ...
2015 CPT Changes Pathology and Laboratory Services
... genomic sequence, must include sequence analysis of entire mitochondrial genome with heteroplasmy detection 81465 Whole mitochondrial genome large deletion analysis panel (eg, Kearns-Sayre syndrome, chronic progressive external ophthalmoplegia), including heteroplasmy detection, if performed 81470 X ...
... genomic sequence, must include sequence analysis of entire mitochondrial genome with heteroplasmy detection 81465 Whole mitochondrial genome large deletion analysis panel (eg, Kearns-Sayre syndrome, chronic progressive external ophthalmoplegia), including heteroplasmy detection, if performed 81470 X ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.