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... rapidly replaced the 15N media with 14N, or light nitrogen. DNA was extracted at various time intervals during the growth of the bacteria, representing different stages of replication (generations). They examined the DNA using density-gradient equilibrium sedimentation and observed that no “heavy” D ...
... rapidly replaced the 15N media with 14N, or light nitrogen. DNA was extracted at various time intervals during the growth of the bacteria, representing different stages of replication (generations). They examined the DNA using density-gradient equilibrium sedimentation and observed that no “heavy” D ...
extracellular polysaccharide colanic acid. cluster responsible for
... were as follows: 621 (59-ATGCCATCTAAAAAACTCTC-39) and 668 (59-AAA GAATTCTCAGCATTGCATCAACGG-39) for the left-hand end of the pPR1742 insert, 667 (59-AAAGAATTCACTGGAACGTACTCAGCC-39) and 679 (59-GGTGCATCACTGCATCCG-39) for the right-hand end of the pPR1742 insert, 622 (59-ATGAAGGTCGGCTTCTTTTTAC-39) and ...
... were as follows: 621 (59-ATGCCATCTAAAAAACTCTC-39) and 668 (59-AAA GAATTCTCAGCATTGCATCAACGG-39) for the left-hand end of the pPR1742 insert, 667 (59-AAAGAATTCACTGGAACGTACTCAGCC-39) and 679 (59-GGTGCATCACTGCATCCG-39) for the right-hand end of the pPR1742 insert, 622 (59-ATGAAGGTCGGCTTCTTTTTAC-39) and ...
Non-Enzymatic, Low Temperature Fluorescence in situ
... A new, low temperature, non-enzymatic in situ hybridization technique (LTFF) that is based on the Fast-FISH technique (Celeda et al., 1994; Durm et al., 1996) was introduced. The aim of this report was the systematic and quantitative analysis of LTFF under different parameters (hybridization tem per ...
... A new, low temperature, non-enzymatic in situ hybridization technique (LTFF) that is based on the Fast-FISH technique (Celeda et al., 1994; Durm et al., 1996) was introduced. The aim of this report was the systematic and quantitative analysis of LTFF under different parameters (hybridization tem per ...
DNA - smoser
... They also discovered that protein-digesting enzymes (proteases) and RNA-digesting enzymes (RNAse) did not affect transformation, so the transforming substance was not a protein or RNA. Digestion with DNase did inhibit transformation, so DNA caused transformation. They concluded that DNA is the here ...
... They also discovered that protein-digesting enzymes (proteases) and RNA-digesting enzymes (RNAse) did not affect transformation, so the transforming substance was not a protein or RNA. Digestion with DNase did inhibit transformation, so DNA caused transformation. They concluded that DNA is the here ...
Isolation of a Complementary DNA Clone for the Human
... sequencing or may represent genetic variants. Several interesting differences between the derived human amino acid sequence and the published sequence for guinea pig C2a were noted. For instance, a Cys at position 18 in the human sequence is Ala in the guinea pig, which may result in a higher order ...
... sequencing or may represent genetic variants. Several interesting differences between the derived human amino acid sequence and the published sequence for guinea pig C2a were noted. For instance, a Cys at position 18 in the human sequence is Ala in the guinea pig, which may result in a higher order ...
Expressing the multifunctional nucleoside kinase of : Drosophila melanogaster Shuba Krishnan
... This study was initiated to investigate a possible strategy to alter an enzyme deficiency in a mouse model. The enzyme investigated is a multifunctional nucleoside kinase from Drosophila melanogaster (Dm-dNK). This enzyme has special features in that it has higher enzymatic activity than any other k ...
... This study was initiated to investigate a possible strategy to alter an enzyme deficiency in a mouse model. The enzyme investigated is a multifunctional nucleoside kinase from Drosophila melanogaster (Dm-dNK). This enzyme has special features in that it has higher enzymatic activity than any other k ...
A gene fusion consisting of 960 base pairs of 5`
... NH^-terminal amino acid sequence was determined by the method of Edman degradation (27). The antino acid sequence analysis (Table 1) showed only one species of interferon molecule with the NH^-terminal sequence: NH^-Glu-Ala-Glu-Ala-Leu-Glu-Phe-Met-Cys-Asp... The sequence was determined by analyzing ...
... NH^-terminal amino acid sequence was determined by the method of Edman degradation (27). The antino acid sequence analysis (Table 1) showed only one species of interferon molecule with the NH^-terminal sequence: NH^-Glu-Ala-Glu-Ala-Leu-Glu-Phe-Met-Cys-Asp... The sequence was determined by analyzing ...
Comparison of Deoxyribonucleic Acid Homologies of Six Strains of
... could be separated from one another based upon differences in polynucleotide sequence homologies and to determine whether there are any polynucleotide sequence homologies among the four genera of ammonia-oxidizing bacteria which we examined. In the past, DNA-DNA hybridization studies involving the a ...
... could be separated from one another based upon differences in polynucleotide sequence homologies and to determine whether there are any polynucleotide sequence homologies among the four genera of ammonia-oxidizing bacteria which we examined. In the past, DNA-DNA hybridization studies involving the a ...
Prolonged organ retention and safety of plasmid DNA
... PEI/DNA complexes (b). Competitive PCR was performed by adding various amounts of IS to the reaction mixtures containing the unknown amounts of sample DNA. Sense and antisense primers were used for coamplification of pCMV and IS. The sense primer was 5⬘TTGACCTCCATAGAAGACACCGG-3⬘ and the antisense p ...
... PEI/DNA complexes (b). Competitive PCR was performed by adding various amounts of IS to the reaction mixtures containing the unknown amounts of sample DNA. Sense and antisense primers were used for coamplification of pCMV and IS. The sense primer was 5⬘TTGACCTCCATAGAAGACACCGG-3⬘ and the antisense p ...
Comparison of Deoxyribonucleic Acid Homologies of Six Strains of
... could be separated from one another based upon differences in polynucleotide sequence homologies and to determine whether there are any polynucleotide sequence homologies among the four genera of ammonia-oxidizing bacteria which we examined. In the past, DNA-DNA hybridization studies involving the a ...
... could be separated from one another based upon differences in polynucleotide sequence homologies and to determine whether there are any polynucleotide sequence homologies among the four genera of ammonia-oxidizing bacteria which we examined. In the past, DNA-DNA hybridization studies involving the a ...
Joseph Jacobson
... visual, tactile, and mental abilities through the digital transmission and processing of all kinds of information. However, all these functions suffer from what has been called 'the tyranny of numbers.' Such systems, because of their complex digital nature, require hundreds, thousands, and sometimes ...
... visual, tactile, and mental abilities through the digital transmission and processing of all kinds of information. However, all these functions suffer from what has been called 'the tyranny of numbers.' Such systems, because of their complex digital nature, require hundreds, thousands, and sometimes ...
Diagnostic molecular testing of microorganisms causing disease in
... This work is copyright. You may download, display, print and reproduce the whole or part of this work in unaltered form for your own personal use or, if you are part of an organisation, for internal use within your organisation, but only if you or your organisation do not use the reproduction for an ...
... This work is copyright. You may download, display, print and reproduce the whole or part of this work in unaltered form for your own personal use or, if you are part of an organisation, for internal use within your organisation, but only if you or your organisation do not use the reproduction for an ...
Protein Synthesis Activity
... 1. On your answer sheet you have a very small segment of a DNA molecule. Use this segment to transcribe a molecule of mRNA. Start transcribing your mRNA molecule when you find “TAC” and stop when you find “ATT”. Remember, each combination of three nitrogenous bases on mRNA is called a codon. 2. Cut ...
... 1. On your answer sheet you have a very small segment of a DNA molecule. Use this segment to transcribe a molecule of mRNA. Start transcribing your mRNA molecule when you find “TAC” and stop when you find “ATT”. Remember, each combination of three nitrogenous bases on mRNA is called a codon. 2. Cut ...
Transformation of Clostridium Thermocellum by Electroporation
... the various engineering schemes that have been used for gene disruption. Electrotransformation, the technique of introducing DNA into a cell by application of an electric field, is a technique that has existed for several decades. Most electrotransformation protocols require five steps: cell growth, ...
... the various engineering schemes that have been used for gene disruption. Electrotransformation, the technique of introducing DNA into a cell by application of an electric field, is a technique that has existed for several decades. Most electrotransformation protocols require five steps: cell growth, ...
Optimizing bacterial expression and purifica- Biomedical laboratory science,
... Despite the discovery of deoxyribonucleic acid (DNA) in the early 1950’s and the subsequent discovery of the structure of DNA by the 1960’s, it was only during the 1970’s, when a new methodology called recombinant DNA technology or genetic engineering was developed. This new methodology made it poss ...
... Despite the discovery of deoxyribonucleic acid (DNA) in the early 1950’s and the subsequent discovery of the structure of DNA by the 1960’s, it was only during the 1970’s, when a new methodology called recombinant DNA technology or genetic engineering was developed. This new methodology made it poss ...
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... test. Final identification for the isolate have been done at two levels: The first was by using conventional method (api 20E) that characterized as the typical easy and rapid one. The second step have been performed by housekeeping gene (rpsL) using polymerase chain reaction technique (PCR) all the ...
... test. Final identification for the isolate have been done at two levels: The first was by using conventional method (api 20E) that characterized as the typical easy and rapid one. The second step have been performed by housekeeping gene (rpsL) using polymerase chain reaction technique (PCR) all the ...
Molecular cloning and characterization of an acyl
... Center for Biotechnology Information; ORF, open reading frame; PCR, polymerase chain reaction; SNP, single nucleotide ...
... Center for Biotechnology Information; ORF, open reading frame; PCR, polymerase chain reaction; SNP, single nucleotide ...
Molecular and General Genetics
... from 94% to 99%) between these genes, and the sequences were equally close to the thyA sequence from B. subtilis 168 and the thyP3 gene from phage /3T. Slight, but signi®cant dierences between the sequences derived from B. subtilis 168, B. amyloliquefaciens S18 and B. atrophaeus S223 on the one han ...
... from 94% to 99%) between these genes, and the sequences were equally close to the thyA sequence from B. subtilis 168 and the thyP3 gene from phage /3T. Slight, but signi®cant dierences between the sequences derived from B. subtilis 168, B. amyloliquefaciens S18 and B. atrophaeus S223 on the one han ...
Phylogenetic analysis of phytoplasmas based on sequences
... upon the 16S rDNA gene which is highly conserved throughout phytoplasma groups. In 2004, the IRPCM Phytoplasma/Spiroplasma Working Team – Phytoplasma taxonomy group proposed to accommodate phytoplasmas within the genus ‘Candidatus Phytoplasma’ gen. nov. in which the formal descriptions and species c ...
... upon the 16S rDNA gene which is highly conserved throughout phytoplasma groups. In 2004, the IRPCM Phytoplasma/Spiroplasma Working Team – Phytoplasma taxonomy group proposed to accommodate phytoplasmas within the genus ‘Candidatus Phytoplasma’ gen. nov. in which the formal descriptions and species c ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.