THE ROLE OF NATURAL SELECTION IN THE ORIGIN OF LIFE
... acid cycle (rTCA cycle) Reduction of carbon provided by CO2 → completion leads to doubling of it's molecules Autocatalytic doubling is a primitive form of reproduction in the model ...
... acid cycle (rTCA cycle) Reduction of carbon provided by CO2 → completion leads to doubling of it's molecules Autocatalytic doubling is a primitive form of reproduction in the model ...
PRADEEP KUMAR BURMA, Ph.D Designation Associate Professor
... 14. Bhullar S, S Datta, S Advani, S Chakravarthy, T Gautam, D Pental and P K Burma. 2007. Functional analysis of Cauliflower Mosaic Virus 35S promoter: re-evaluation of the role of subdomains B5, B4 and B5 in promoter activity. Plant Biotechnology Journal. 5: 696-708. 15. Bisht N C, A Jagannath, P K ...
... 14. Bhullar S, S Datta, S Advani, S Chakravarthy, T Gautam, D Pental and P K Burma. 2007. Functional analysis of Cauliflower Mosaic Virus 35S promoter: re-evaluation of the role of subdomains B5, B4 and B5 in promoter activity. Plant Biotechnology Journal. 5: 696-708. 15. Bisht N C, A Jagannath, P K ...
Evidence That Plant K+ Channel Proteins Have
... A full-length cDNA encoding the bovine brain /3 subunit (KJ2) was recently cloned (Scott et al., 1994) and used to screen a rat brain cDNA library (Rettig et al., 1994). Two clones showing sequence homology were identified: rat K,Pl and rat K$2. One of the rat cDNAs, KvP2, encodes a deduced amino ac ...
... A full-length cDNA encoding the bovine brain /3 subunit (KJ2) was recently cloned (Scott et al., 1994) and used to screen a rat brain cDNA library (Rettig et al., 1994). Two clones showing sequence homology were identified: rat K,Pl and rat K$2. One of the rat cDNAs, KvP2, encodes a deduced amino ac ...
Phaeospirillum oryzae sp. nov., a spheroplast
... evolution methods in the MEGA 4 software (http://www. megasoftware.net/mega4/mega.html) were used to reconstruct phylogenetic trees. Percentage support values were obtained using a bootstrap procedure. The taxonomic relationship between strains JA317T and JA559 and other species of the genus Phaeosp ...
... evolution methods in the MEGA 4 software (http://www. megasoftware.net/mega4/mega.html) were used to reconstruct phylogenetic trees. Percentage support values were obtained using a bootstrap procedure. The taxonomic relationship between strains JA317T and JA559 and other species of the genus Phaeosp ...
DN A:The Master Molecule of Life
... DNA molecule is actually made up of two separate strands that wind around and around one another creating a shape called a double helix. Each of the two DNA strands is made up of smaller chemical subunits called nucleotides which are joined together, one after another, in a very precise order.Only f ...
... DNA molecule is actually made up of two separate strands that wind around and around one another creating a shape called a double helix. Each of the two DNA strands is made up of smaller chemical subunits called nucleotides which are joined together, one after another, in a very precise order.Only f ...
Regulation of metabolic products and gene expression in Fusarium
... (Agilent Technologies). In this process, linear and LOWESS normalizations were carried out to remove the dye biases of the data. Genes classified as induced or reduced were those passing a sample t-test (P<0.05). The RNAs extracted from three independent cultures were measured separately. Metabolome ...
... (Agilent Technologies). In this process, linear and LOWESS normalizations were carried out to remove the dye biases of the data. Genes classified as induced or reduced were those passing a sample t-test (P<0.05). The RNAs extracted from three independent cultures were measured separately. Metabolome ...
(you should!). What exactly is the role of DNA and h
... So, the sequence of bases in DNA codes for the sequence of amino acids of a protein. But, there's a problem. In order to produce a protein you need ribosomes. Ribosomes catalyse the reactions of protein synthesis and if you can remember back to the first topic you'll know that ribosomes are found in ...
... So, the sequence of bases in DNA codes for the sequence of amino acids of a protein. But, there's a problem. In order to produce a protein you need ribosomes. Ribosomes catalyse the reactions of protein synthesis and if you can remember back to the first topic you'll know that ribosomes are found in ...
An improved technique for isolating codominant compound
... SSR markers, this approach substantially reduced the cost of developing codominant markers and analyzing their polymorphism. We have demonstrated this technique for Dendropanax trifidus and easily developed 11 codominant markers with high polymorphism for D. trifidus. Use of the technique for succes ...
... SSR markers, this approach substantially reduced the cost of developing codominant markers and analyzing their polymorphism. We have demonstrated this technique for Dendropanax trifidus and easily developed 11 codominant markers with high polymorphism for D. trifidus. Use of the technique for succes ...
AZT resistance of simian foamy virus reverse transcriptase is based
... Horiba Fluoromax fluorimeter equipped with an automatic titration device (Hamilton). Following sample equilibration, at least six data points with an integration time of 1 s were collected for each titration point. Data fitting. Data were fitted to a two-component binding equation to determine the equ ...
... Horiba Fluoromax fluorimeter equipped with an automatic titration device (Hamilton). Following sample equilibration, at least six data points with an integration time of 1 s were collected for each titration point. Data fitting. Data were fitted to a two-component binding equation to determine the equ ...
Chapter 21 Nucleic Acids and Protein Synthesis
... mRNA Processing: Exons and Introns The DNA of eukaryotes contains exons that code for proteins along with introns that do not. The initial mRNA called a pre-RNA includes the noncoding introns. While in the nucleus, the introns are removed from the pre-RNA. The exons that remain are joined t ...
... mRNA Processing: Exons and Introns The DNA of eukaryotes contains exons that code for proteins along with introns that do not. The initial mRNA called a pre-RNA includes the noncoding introns. While in the nucleus, the introns are removed from the pre-RNA. The exons that remain are joined t ...
Protocol for RiboShredder™ RNase Blend
... of applications. When used for DNA purification, all unwanted RNA can be removed using a simple 10-minute procedure. After the reaction is complete, RiboShredder RNase Blend can be removed using a phenol-chloroform extraction procedure. It’s broad range of salt tolerance makes it ideal for use in ma ...
... of applications. When used for DNA purification, all unwanted RNA can be removed using a simple 10-minute procedure. After the reaction is complete, RiboShredder RNase Blend can be removed using a phenol-chloroform extraction procedure. It’s broad range of salt tolerance makes it ideal for use in ma ...
1 Single molecule sequencing of THCA synthase reveals
... intermittent haplogroups. We cannot rule out the possibility of other diverged copies of inactive THCAS stochastically amplifying given the PCR reactions were set up independently. Gradient PCR at lower annealin ...
... intermittent haplogroups. We cannot rule out the possibility of other diverged copies of inactive THCAS stochastically amplifying given the PCR reactions were set up independently. Gradient PCR at lower annealin ...
Parallel Analysis of Gene Expression: Microarrays
... described below, there are currently three major types of platform: cDNA and longoligonucleotide microarrays, and short-oligonucleotide gene chips. Other technologies based on beads and three-dimensional arrays are just emerging. Major considerations involved in selecting a platform include cost (fr ...
... described below, there are currently three major types of platform: cDNA and longoligonucleotide microarrays, and short-oligonucleotide gene chips. Other technologies based on beads and three-dimensional arrays are just emerging. Major considerations involved in selecting a platform include cost (fr ...
Transcription (genetics)
... (5-carbon) sugar where DNA has deoxyribose (one less oxygen atom) in its sugar-phosphate backbone). Unlike DNA replication, mRNA transcription can involve multiple RNA polymerases on a single DNA template and multiple rounds of transcription (amplification of particular mRNA), so many mRNA molecules ...
... (5-carbon) sugar where DNA has deoxyribose (one less oxygen atom) in its sugar-phosphate backbone). Unlike DNA replication, mRNA transcription can involve multiple RNA polymerases on a single DNA template and multiple rounds of transcription (amplification of particular mRNA), so many mRNA molecules ...
PHYLOGENETIC ANALYSIS AND HOMOLOGY MODELLING OF
... Morphological identification of fungi is the first and the most difficult step in the identification process. This is especially true for Fusarium species. Fusarium has a cosmopolitan distribution. Although morphological observations may not suffice for complete identification, a great deal of infor ...
... Morphological identification of fungi is the first and the most difficult step in the identification process. This is especially true for Fusarium species. Fusarium has a cosmopolitan distribution. Although morphological observations may not suffice for complete identification, a great deal of infor ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
... colony colour, utilization of D-fructose, myo-inositol, Dmannitol and D-mannose) they should be considered as members of a single species. According to Stackebrandt (2011), the level of genome sequence identity among two strains must be higher than 96 % to reach a DDH similarity value of higher than ...
... colony colour, utilization of D-fructose, myo-inositol, Dmannitol and D-mannose) they should be considered as members of a single species. According to Stackebrandt (2011), the level of genome sequence identity among two strains must be higher than 96 % to reach a DDH similarity value of higher than ...
Chpt3_Isolating_analyzing_genes.doc
... The first two chapters covered many important aspects of genes, such as how they function in inheritance, how they code for protein (in general terms) and their chemical nature. All this was learned without having a single gene purified. A full understanding of a gene, or the entire set of genes in ...
... The first two chapters covered many important aspects of genes, such as how they function in inheritance, how they code for protein (in general terms) and their chemical nature. All this was learned without having a single gene purified. A full understanding of a gene, or the entire set of genes in ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
... colony colour, utilization of D-fructose, myo-inositol, Dmannitol and D-mannose) they should be considered as members of a single species. According to Stackebrandt (2011), the level of genome sequence identity among two strains must be higher than 96 % to reach a DDH similarity value of higher than ...
... colony colour, utilization of D-fructose, myo-inositol, Dmannitol and D-mannose) they should be considered as members of a single species. According to Stackebrandt (2011), the level of genome sequence identity among two strains must be higher than 96 % to reach a DDH similarity value of higher than ...
Functional dissection of the baculovirus late expression factor
... viral genes are transcribed during three phases, designated early, late and very late. Early genes are transcribed prior to viral DNA replication by the host RNA polymerase II and host transcription accessory factors and, in some cases, also require specific virus factors. Their promoter regions res ...
... viral genes are transcribed during three phases, designated early, late and very late. Early genes are transcribed prior to viral DNA replication by the host RNA polymerase II and host transcription accessory factors and, in some cases, also require specific virus factors. Their promoter regions res ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.