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Page 1 United States Patent [19] Anderson et al
... tion of relatively short oligonucleotides to single-stranded ...
... tion of relatively short oligonucleotides to single-stranded ...
Supplemental figure 1 Complete CLSM stacks of Ad3 texas
... the Eco RV-linearised form of the three plasmids ppolyAd∆EP-TETP, ppoly-Ad∆EPTETP-∆24, ppoly-Ad∆EP-TETP-∆24∆19 and a modified form of the 28 kb Cla I- Pac I fragment containing a Swa I restriction site inserted into the deleted fiber region 7. The second recombination was between the Swa I-linearise ...
... the Eco RV-linearised form of the three plasmids ppolyAd∆EP-TETP, ppoly-Ad∆EPTETP-∆24, ppoly-Ad∆EP-TETP-∆24∆19 and a modified form of the 28 kb Cla I- Pac I fragment containing a Swa I restriction site inserted into the deleted fiber region 7. The second recombination was between the Swa I-linearise ...
Bacterial genome replication at subzero temperatures in permafrost
... microbial activity may be due to basal metabolism or macromolecular repair, there is also ample evidence for cellular growth. Unfortunately, most metabolic measurements or culture-based laboratory experiments cannot elucidate the specific microorganisms responsible for metabolic activities in native ...
... microbial activity may be due to basal metabolism or macromolecular repair, there is also ample evidence for cellular growth. Unfortunately, most metabolic measurements or culture-based laboratory experiments cannot elucidate the specific microorganisms responsible for metabolic activities in native ...
The Ames Test
... mutant bacteria so we can determine what types of mutations were induced. The Assay: Since DNA is chemically the same in all organisms, any living organism can be used to test for mutagens. Thus, bacteria can be used as a first step in identifying potential human carcinogens without waiting for long ...
... mutant bacteria so we can determine what types of mutations were induced. The Assay: Since DNA is chemically the same in all organisms, any living organism can be used to test for mutagens. Thus, bacteria can be used as a first step in identifying potential human carcinogens without waiting for long ...
CAMPYLOBACTER
... Adhesion and Invasion Assaying by HEp-2 cells, clinical isolates of C. jejuni were more invasive than the nonclinical strains studied When HEp-2 cells were treated with cytochalasin B, the invasiveness of C. jejuni was reduced, indicating active participation of the host cell in the uptake of t ...
... Adhesion and Invasion Assaying by HEp-2 cells, clinical isolates of C. jejuni were more invasive than the nonclinical strains studied When HEp-2 cells were treated with cytochalasin B, the invasiveness of C. jejuni was reduced, indicating active participation of the host cell in the uptake of t ...
Molecular cloning and characterization of cm3 gene, from t
... wild emmer wheat showed a wide range of diversity in WDAI, WMAI both between and within populations, he also found that alpha -amylase inhibitors are adaptively selected under different environments according to population and codon analysis. However in the present study no significant changes in CM ...
... wild emmer wheat showed a wide range of diversity in WDAI, WMAI both between and within populations, he also found that alpha -amylase inhibitors are adaptively selected under different environments according to population and codon analysis. However in the present study no significant changes in CM ...
Unusual C-terminal domain of the largest subunit of RNA
... considerably from this consensus sequence, but the basic structure observed in other higher eukaryotes is still present (reviewed in (2,10). Three characteristics of the C-terminal domain in eukaryotes suggest that this region might form a unique secondary structure: (i) the amino acid composition, ...
... considerably from this consensus sequence, but the basic structure observed in other higher eukaryotes is still present (reviewed in (2,10). Three characteristics of the C-terminal domain in eukaryotes suggest that this region might form a unique secondary structure: (i) the amino acid composition, ...
Manual: Universal Human miRNA Reference RNA
... Stratagene Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis ...
... Stratagene Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis ...
Electrochemical detection of polymerase reactions by specific metal
... [0021] The inventors have unexpectedly found that biological reactions which release pyrophosphate may be detected by electrochemical methods via the formation of pyrophosphate/metal ion complexes and/or precipitates. [0022] The present invention is based on the principle that pyrophosphate (PPi; in ...
... [0021] The inventors have unexpectedly found that biological reactions which release pyrophosphate may be detected by electrochemical methods via the formation of pyrophosphate/metal ion complexes and/or precipitates. [0022] The present invention is based on the principle that pyrophosphate (PPi; in ...
Gene Mutations Worksheet
... 1. Review with the class about point mutations and the differences between frame shift and base substitution. 2. Students work on the handout by themselves. Accommodations: Students with an IEP can take the handout home if they need extra time, and/or do questions 1 - 3 and questions 11 - 24. Evalua ...
... 1. Review with the class about point mutations and the differences between frame shift and base substitution. 2. Students work on the handout by themselves. Accommodations: Students with an IEP can take the handout home if they need extra time, and/or do questions 1 - 3 and questions 11 - 24. Evalua ...
The sequence of the tms transcript 2 locus of the A. tumefaciens
... reading frame we have detected (see below). The promoter for transcription was apparently within Hind III fragment 22e. Schroder,et. al.(34) have also shown that coupled j ^ vitro transcription/translation systems prepared from both J^ coli and A_^ tumefaciens express the 49 Kd protein, albeit poorl ...
... reading frame we have detected (see below). The promoter for transcription was apparently within Hind III fragment 22e. Schroder,et. al.(34) have also shown that coupled j ^ vitro transcription/translation systems prepared from both J^ coli and A_^ tumefaciens express the 49 Kd protein, albeit poorl ...
For the last three and a half billion years, evolution has been
... documentation represents the shared rules that allow the three databases to exchange data on a daily basis. The range of features to be represented is diverse, including regions which: perform a biological function, affect or are the result of the expression of a biological function, ...
... documentation represents the shared rules that allow the three databases to exchange data on a daily basis. The range of features to be represented is diverse, including regions which: perform a biological function, affect or are the result of the expression of a biological function, ...
Synthesis and characterization of glycoconjugate tin(IV) complexes
... a direct relationship between their interactions with macromolecules, hence, leading to their therapeutic effect [8,9]. There is considerable promise in enhancing the targeting and efficiency of metal-chelators through ligand design. Ligands can significantly alter the biological properties by modifyi ...
... a direct relationship between their interactions with macromolecules, hence, leading to their therapeutic effect [8,9]. There is considerable promise in enhancing the targeting and efficiency of metal-chelators through ligand design. Ligands can significantly alter the biological properties by modifyi ...
... rate of chemical reactions. Provide one example of a reaction mechanism to illustrate your answer. Choice B: Enzymes are specific for their substrates. How is substrate specificity achieved by enzymes? Illustrate your answer by discussing two enzymes with different substrate specificities. Choice A: ...
Expression and purification of four different rhizobial acyl carrier
... of the different rhizobial ACPs will allow detailed structural investigations. Also, the different rhizobial ACPs will be invaluable tools for comparative biochemical studies in which acyl-ACPs are used as cosubstrates. In particular, the constitutive AcpP of S. meliloti will be required to elucidat ...
... of the different rhizobial ACPs will allow detailed structural investigations. Also, the different rhizobial ACPs will be invaluable tools for comparative biochemical studies in which acyl-ACPs are used as cosubstrates. In particular, the constitutive AcpP of S. meliloti will be required to elucidat ...
pdf format - Faculty members Homepages
... the human genome based on their apparent lack of intron sequences on chromosomes 1, 10, and 11. The first 164 aa of HDAC4 have a perfect match on chromosome 3, and part of the HDAC9 HDAC domain has a perfect match on chromosome 18. Other HDAC family members have isoforms (10). Of the eight class I a ...
... the human genome based on their apparent lack of intron sequences on chromosomes 1, 10, and 11. The first 164 aa of HDAC4 have a perfect match on chromosome 3, and part of the HDAC9 HDAC domain has a perfect match on chromosome 18. Other HDAC family members have isoforms (10). Of the eight class I a ...
Evolutionary Adaptation to Different Thermal Environments via
... Fundulus heteroclitus from Bar Harbor, Maine, and Sapelo Island, Ga., were acclimated to 20°C 15 parts/ 1,000 parts of seawater, 14:lO lightdark cycle for a minimum of 24 wk. All assays were done in pairs, one member from each population. All assays used liver tissue. Liver only expresses the Ldh-B ...
... Fundulus heteroclitus from Bar Harbor, Maine, and Sapelo Island, Ga., were acclimated to 20°C 15 parts/ 1,000 parts of seawater, 14:lO lightdark cycle for a minimum of 24 wk. All assays were done in pairs, one member from each population. All assays used liver tissue. Liver only expresses the Ldh-B ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.