Supplementary Information (doc 82K)
... ribosomal subunits, 80S monosome and polysome peaks are indicated. (A, B bottom panels) Effect of temperature stress on sense (s) and antisense (as) LSU γ rRNA fragmentation. Total RNA extracted from unstressed and temperature-stressed L. infantum promastigotes was isolated from sucrose gradient fra ...
... ribosomal subunits, 80S monosome and polysome peaks are indicated. (A, B bottom panels) Effect of temperature stress on sense (s) and antisense (as) LSU γ rRNA fragmentation. Total RNA extracted from unstressed and temperature-stressed L. infantum promastigotes was isolated from sucrose gradient fra ...
Chance and Necessity in the Selection of Nucleic Acid Catalysts
... 100-mers can fold into structures capable of specifically binding certain organic dyes.4 In the last few years a number of RNA and DNA aptamers for amino acids,5-9 drugs,10 and enzymatic cofactors8,11-15 have been isolated. Typical association constants, measured in aqueous buffers, are in the range ...
... 100-mers can fold into structures capable of specifically binding certain organic dyes.4 In the last few years a number of RNA and DNA aptamers for amino acids,5-9 drugs,10 and enzymatic cofactors8,11-15 have been isolated. Typical association constants, measured in aqueous buffers, are in the range ...
Nucleic Acids
... account for the observed thickness, whereas a pair of purine bases is too large. Thus, according to the Watson–Crick model, the repeating units in a double-stranded DNA molecule are not single bases of differing dimensions, but rather base pairs of almost identical dimensions. To account for the per ...
... account for the observed thickness, whereas a pair of purine bases is too large. Thus, according to the Watson–Crick model, the repeating units in a double-stranded DNA molecule are not single bases of differing dimensions, but rather base pairs of almost identical dimensions. To account for the per ...
Membrane Protein Expression in Cell
... approach requires four primers as illustrated. First, a PCR product is generated with equal amounts of primers 3 and 4 (10 nM each). This product is then used as template in a second PCR reaction with a 100-fold excess of primers 1 and 4 with respect to primer 2 (100 to 1 nM ). ...
... approach requires four primers as illustrated. First, a PCR product is generated with equal amounts of primers 3 and 4 (10 nM each). This product is then used as template in a second PCR reaction with a 100-fold excess of primers 1 and 4 with respect to primer 2 (100 to 1 nM ). ...
R4, a non-LTR retrotransposon specific to the
... genes of the host simplifies their identification in other species by either Southern blotting or PCR amplification (1,18). PCR is the more sensitive of the two approaches and we are currently able to detect R1 and/or R2 elements in insects that we previously scored by Southern analysis as being neg ...
... genes of the host simplifies their identification in other species by either Southern blotting or PCR amplification (1,18). PCR is the more sensitive of the two approaches and we are currently able to detect R1 and/or R2 elements in insects that we previously scored by Southern analysis as being neg ...
Fish-on-a-chip: a sensitive detection microfluidic system for
... brain diseases such as AD, which are present in the human brain and are associated with chromosomal disorders [40-42]. In this research, chromosome 21 aneuploidy in lymphocytes and fibroblasts cells of AD patients was observed using FISH techniques [43-45]. In this study, three kinds of DNA probes w ...
... brain diseases such as AD, which are present in the human brain and are associated with chromosomal disorders [40-42]. In this research, chromosome 21 aneuploidy in lymphocytes and fibroblasts cells of AD patients was observed using FISH techniques [43-45]. In this study, three kinds of DNA probes w ...
Characteristics of the gene encoding pyrroline-5-carboxylate synthase (P5CS) in Glycine max
... products with primer pairs with expectant size is 400 bp, respectively, 700 bp and 1770 bp. PCR products were detected by electrophoresis on 0.8% agarose gel, the results showed that the obtained DNA fragments have the match sizes with the theoretical calculated. Product of PCR include three DNA fr ...
... products with primer pairs with expectant size is 400 bp, respectively, 700 bp and 1770 bp. PCR products were detected by electrophoresis on 0.8% agarose gel, the results showed that the obtained DNA fragments have the match sizes with the theoretical calculated. Product of PCR include three DNA fr ...
Chapter 6 Pichia pastoris
... source. This is achieved by the oxidation of methanol to formaldehyde using molecular oxygen by the enzyme alcohol oxidase (AOX). The promoter regulating the production of alcohol oxidase is the one used to drive the MeOH-induced protein expression in Pichia (Invitrogen Corporation, 2001). Two genes ...
... source. This is achieved by the oxidation of methanol to formaldehyde using molecular oxygen by the enzyme alcohol oxidase (AOX). The promoter regulating the production of alcohol oxidase is the one used to drive the MeOH-induced protein expression in Pichia (Invitrogen Corporation, 2001). Two genes ...
KINE 4010 Mock Midterm #1
... b) Anaerobic glycolysis is occurring, which leads to acidosis c) The muscles are beginning to break down, which is causing high lactic acid in the blood d) None of the above 23. What is the main difference between a 15,000-meter run Marathon runner and a 100meter dash runner? a) The 15,000-meter mar ...
... b) Anaerobic glycolysis is occurring, which leads to acidosis c) The muscles are beginning to break down, which is causing high lactic acid in the blood d) None of the above 23. What is the main difference between a 15,000-meter run Marathon runner and a 100meter dash runner? a) The 15,000-meter mar ...
Plant Physiology
... containing periderm cells. This pattern of expression more closely resembles that of ENOD2 in Sesbania rostrata nodules (Goormachtig et al., 1998b), and of ENOD40 in soybean nodules (Yang et al., 1993). In soybean, GmENOD93 transcripts were detectable as early as 3 d following incubation with Bradyr ...
... containing periderm cells. This pattern of expression more closely resembles that of ENOD2 in Sesbania rostrata nodules (Goormachtig et al., 1998b), and of ENOD40 in soybean nodules (Yang et al., 1993). In soybean, GmENOD93 transcripts were detectable as early as 3 d following incubation with Bradyr ...
1 - Plant Research International
... The genes responsible for the pathway enzymes will be identified directly using the techniques of differential display (P3). The added advantage of these studies is that it leads into the possible genetic manipulation of the pathway. In the technique of differential display mRNA is used to produce a ...
... The genes responsible for the pathway enzymes will be identified directly using the techniques of differential display (P3). The added advantage of these studies is that it leads into the possible genetic manipulation of the pathway. In the technique of differential display mRNA is used to produce a ...
Chapter Seventeen: Gene Mutations and DNA Repair
... or vice versa. Although transversions would seem to be statistically favored because there are eight possible transversions and only four possible transitions, about twice as many transition mutations are actually observed in the human genome. *3. Briefly describe expanding trinucleotide repeats. Ho ...
... or vice versa. Although transversions would seem to be statistically favored because there are eight possible transversions and only four possible transitions, about twice as many transition mutations are actually observed in the human genome. *3. Briefly describe expanding trinucleotide repeats. Ho ...
From DNA sequence to application: possibilities and
... 2D gel electrophoresis to construct a 2D protein index as has been dolle for B. subtilis (Bernhardt et al. 1997; Schmid et al. 1997; ). However, to link particular protein spots to the corresponding gelles is time-consuming and often requires microsequencing and/or mutant production. Moreover, gelle ...
... 2D gel electrophoresis to construct a 2D protein index as has been dolle for B. subtilis (Bernhardt et al. 1997; Schmid et al. 1997; ). However, to link particular protein spots to the corresponding gelles is time-consuming and often requires microsequencing and/or mutant production. Moreover, gelle ...
RNA Structure
... XXIII. What Are the Different Classes of Nucleic Acids? [S23] a. Two kinds of nucleic acids: i. DNA – only one type, only serves one purpose, which is storing your genetic information. ii. RNA – there are 4 different types and they serve different purposes 1. The first one is the focus of our lectur ...
... XXIII. What Are the Different Classes of Nucleic Acids? [S23] a. Two kinds of nucleic acids: i. DNA – only one type, only serves one purpose, which is storing your genetic information. ii. RNA – there are 4 different types and they serve different purposes 1. The first one is the focus of our lectur ...
Insulin-like growth factor binding protein-2 (IGFBP
... 30 s. A melting curve was collected using two additional cycles by reading the fluorescence value from 65 °C to 95 °C, in order to assess the specificity of the PCR amplification. Negative controls contained non-template controls (NTC), and interplate calibrator (IPC) were performed. For normalizati ...
... 30 s. A melting curve was collected using two additional cycles by reading the fluorescence value from 65 °C to 95 °C, in order to assess the specificity of the PCR amplification. Negative controls contained non-template controls (NTC), and interplate calibrator (IPC) were performed. For normalizati ...
Nucleic acid crystallography: current progress
... over the past two years makes clear the incredibly versatile nature of nucleic acid tertiary structure. Long gone are the days when it was possible to group nucleic acid crystal structures simply into A-, B- and Z-form DNA double helices [3]. But even in the case of double helical fragments, it is n ...
... over the past two years makes clear the incredibly versatile nature of nucleic acid tertiary structure. Long gone are the days when it was possible to group nucleic acid crystal structures simply into A-, B- and Z-form DNA double helices [3]. But even in the case of double helical fragments, it is n ...
Strategies for Attaching Oligonucleotides to Solid Supports
... reactions for each SNP a pair of probes are designed which differ from each other at their extreme 3' nucleotide (the polymorphic site). In the presence of DNA polymerase, dNTPs and a small portion of the biotin labeled dCTP, a labeled extension product of the 3' portion of the primer is obtained on ...
... reactions for each SNP a pair of probes are designed which differ from each other at their extreme 3' nucleotide (the polymorphic site). In the presence of DNA polymerase, dNTPs and a small portion of the biotin labeled dCTP, a labeled extension product of the 3' portion of the primer is obtained on ...
DNA polymerase active site is highly mutable
... of E. coli recA718 polA12. This E. coli strain, which contains a temperature-sensitive mutation in the polA gene, which encodes DNA polymerase I, forms colonies at 30°C, but not at 37°C. After selection, we find DNA polymerase catalytic site (motif A) is in fact highly mutable while preserving wild- ...
... of E. coli recA718 polA12. This E. coli strain, which contains a temperature-sensitive mutation in the polA gene, which encodes DNA polymerase I, forms colonies at 30°C, but not at 37°C. After selection, we find DNA polymerase catalytic site (motif A) is in fact highly mutable while preserving wild- ...
Supporting information S1.
... Supporting information S1. Detailed explanation of plasmids and strains construction The suicide vector pKNG101 was used to introduce the CAT* reporter gene within the Escherichia coli chromosome (Table S2). This plasmid contains a defective pir minus origin of replication (oriR6K), the strAB genes ...
... Supporting information S1. Detailed explanation of plasmids and strains construction The suicide vector pKNG101 was used to introduce the CAT* reporter gene within the Escherichia coli chromosome (Table S2). This plasmid contains a defective pir minus origin of replication (oriR6K), the strAB genes ...
A structural determinant in the uracil DNA glycosylase superfamily
... Taq DNA polymerase, was initially carried out with a predenaturation at 95◦ C for 2 min, five cycles with each cycle of denaturation at 95◦ C for 30 s and annealing and extension at 60◦ C for 4 min, and a final extension at 72◦ C for 5 min. Afterward, 100 nM outside primers (UDG-NdeI and UDG-HindIII ...
... Taq DNA polymerase, was initially carried out with a predenaturation at 95◦ C for 2 min, five cycles with each cycle of denaturation at 95◦ C for 30 s and annealing and extension at 60◦ C for 4 min, and a final extension at 72◦ C for 5 min. Afterward, 100 nM outside primers (UDG-NdeI and UDG-HindIII ...
RIBOSWITCHES - Creighton Chemistry Webserver
... RNA domains that modulate gene expression in response to metabolite binding ...
... RNA domains that modulate gene expression in response to metabolite binding ...
Binding of the EcoRII methyltransferase to 5
... Mbol and Main sites. To prove that the original polymer was a repeating polymer containing this sequence it was digested with each of these restriction enzymes. The digests each yielded one fragment having identical mobilities on electrophoresis (figure 1). The basis for the synthesis of this polyme ...
... Mbol and Main sites. To prove that the original polymer was a repeating polymer containing this sequence it was digested with each of these restriction enzymes. The digests each yielded one fragment having identical mobilities on electrophoresis (figure 1). The basis for the synthesis of this polyme ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.